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Biomedical subjects

M R Harris

Publications and source records attributed to M R Harris.

At least 55 records · Page 3Linked to original sources
PubMed1984

Antigen presentation by the BCL1 murine B cell line: in vitro stimulation by LPS.

We examined the antigen-presenting capacity of BCL1 tumor cells, which are capable of differentiating in vitro with respect to immunoglobulin synthesis/secretion under the influence of LPS. In vivo passaged BCL1 cells depleted of host cell contamination either by positive selection employing panning with anti-lambda reagents, or by elimination of latex-ingesting adherent cells, are capable of MHC-restricted antigen presentation to a GAT-immune T cell line. The BCL1 cells act as antigen-presenting cells when freshly explanted, but gradual loss of this function occurs, and cells cultured for 3.5 days cannot present antigen unless LPS is included during the culture period. BCL1 cells are equivalently Ia+ after the culture period with or without LPS stimulation. Other B cell lines capable of antigen presentation appear to express this trait constitutively, and the in vivo passaged BCL1 line is therefore unique among B cell lines in having antigen-presenting cell function that can be modulated. The data suggest that freshly explanted or LPS-cultured BCL1 cells are heterogeneous with respect to antigen-presenting capacity, and the basis for this heterogeneity is being sought. BCL1 offers an opportunity to study requirements for antigen presentation by B cells.

Animals↗
PubMed1983

A qualitative and quantitative study of subfractions of the histone H10 in various mammalian tissues.

The histone H10 was examined from seven mammalian species. All tissues were shown to contain two subfractions of H10, except for those of rabbit, in which little or no H10 was found. The subfraction composition was compared quantitatively in different mouse and hamster tissues, with the conclusion that this composition is tissue-specific. It is proposed that the wide occurrence of H10, together with the evidence of no more or less than two subfractions wherever it occurs, and the tissue-specific nature of the ratio of subfractions, signify that these two subfractions have specific individual functions.

Animals↗
PubMed1976

The attitudes of nursing students and others about mental illness.

Student nurses tend to report somewhat moderate attitudes toward mental illness in that they appear to be less radical than psychology students, but less conservative than teacher-students in education. The student nurses' attitudes are similar to those of psychiatric residents, but more radical than psychiatric nurses.

Attitude of Health Personnel↗
PubMed1969

The hydrolysis of uridine cyclic phosphonate catalyzed by ribonuclease-A: implications for the mechanism of action of the enzyme.

Uridine 2',3'-cyclic phosphonate (I) is slowly hydrolyzed by ribonuclease-A with k(2) and K(m) values at pH6 that are respectively 1900 and 15 times smaller than the same parameters at the same pH for the related phosphate (II). Since the ratio of rate constants for hydroxide ion catalyzed hydrolysis is about 4, this result is consistent with, but does not prove a mechanism for the enzymic reaction that requires a pseudorotation.

Kinetics↗