Search PubMed⌕ Search

Biomedical subjects

M R Harris

Publications and source records attributed to M R Harris.

At least 37 records · Page 2Linked to original sources
PubMed1997

Codes of ethics and scientific integrity: what relevance to outcomes activities?

Advanced practice nurses are involved in many activities that focus around "outcomes." As nurses participate in the public presentation of outcomes data and information, expectations for ethical and responsible behavior are heightened. This article examines dilemmas that exist for advanced practice nurses involved in outcomes activities within the context of the American Nurses Association code for nurses, the bioethics literature, and published guides to scientific integrity. Recommendations are made to address these dilemmas.

Ethics, Nursing↗
PubMed1996

Quantitative evaluation of dizziness characteristics and impact on quality of life.

Patients attending an outpatient clinic with the complaint of dizziness were given a set of five items to rank the magnitude of the frequency and severity of their dizziness and the impact of dizziness on their quality of life. They were given instruments to evaluate their degree of anxiety and depression. The impact of frequency and severity on the patients' daily activities, on the quality of life, and on the fear of becoming dizzy was self-rated by the patient. The score on quality of life was compared with that provided by the physician in a blind questionnaire. The items that were developed addressed the physical, functional, and emotional impact of dizziness and can be considered promising for the evaluation of the patients' degree of overall impairment.

Adolescent↗
PubMed1995

Patient outcomes: assessment issues for the CNS.

Patient outcomes are becoming markers of health care effectiveness and quality. The CNS is uniquely positioned to influence outcomes evaluation within nursing. One way to accomplish this is through recommendations on the selection and use of appropriate patient assessment tools within the patient database. Recommendations of the CNS should be based on: an awareness of patient outcomes of particular interest to nursing; a goal of standardized, retrievable clinical data; characteristics of clinically appropriate assessment tools for outcomes evaluation; and data needs of patient outcomes research as well as traditional clinical research.

Humans↗
PubMed1994

A model of transitive choice.

We report a formal model of transitive inference based on protocols from experiments on squirrel monkeys solving the 5-term series problem (McGonigle & Chalmers, 1977, 1992). These studies generate databases featuring transitive choice, task transfer (where at first a significant decrement is observed, and later substantial improvement without explicit training), and, finally, a Symbolic Distance Effect (SDE) based on decision-time data. Using a rule-based (production) system, we first established rule stacks at the group, then at the individual level, on the basis of triadic transfer performance first recorded in the McGonigle and Chalmers (1977) study. The models for each subject then accommodated data from the more intensive, later study with the same subjects (McGonigle & Chalmers, 1992). We found the initial model capable of dealing with all choice and reaction-time phenomena reported thus far, with only small changes in a rule search procedure. In common with an independent assay by McGonigle and Chalmers (1992), our model-based reassessment of decision times indicates that a major source of reaction time variation is item prominence in the rule stack rather than interitem (ordinal) distance per se.

Animals↗
PubMed1994

Fibre optic confocal imaging (FOCI) for subsurface microscopy of the colon in vivo.

Fibre optic confocal imaging (FOCI) is a new type of microscopy which has been recently developed (Delaney et al. 1993). In contrast to conventional light microscopy, FOCI and other confocal techniques allow clear imaging of subsurface structures within translucent objects. However, unlike conventional confocal microscopes which are bulky (because of a need for accurate alignment of large components) FOCI allows the imaging end to be miniaturised and relatively mobile. FOCI is thus particularly suited for clear subsurface imaging of structures within living animals or subjects. The aim of the present study was to assess the suitability of using FOCI for imaging of subsurface structures within the colon, both in vitro (human and rat biopsies) and in vivo (in rats). Images were obtained in fluorescence mode (excitation 488 nm, detection above 515 nm) following topical application of fluorescein. By this technique the glandular structure of the colon was imaged. FOCI is thus suitable for subsurface imaging of the colon in vivo.

Animals↗
PubMed1993

PLIM: a protein-ligand interaction modeller.

A computer program is presented that models the binding of selected chemical groups to a protein surface. The groups are successively incorporated at energetically favourable positions to build up a pharmacophore pattern that may be used as the basis for a database search for possible ligands. The ability to predict known binding points in a trypsin-inhibitor complex is demonstrated, and the results from a run on dihydrofolate reductase are shown to be usable as a pharmacophore pattern for a database search.

Animals↗
PubMed1991

Structure of the pig insulin dimer in the cubic crystal.

Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1.7 A resolution with structure-factor amplitudes greater than two standard deviations. Stereochemical parameters for the refined model are close to standard values and the estimated error in the positions of well-ordered atoms is about 0.1 A. Residues directly involved in the formation of the exact (crystallographic) cubic insulin dimer are oriented similarly to those in the non-crystallographic 2Zn insulin dimer. Other residues, which make different molecular contacts in the different crystal forms, have locally altered conformations. The cubic insulin molecule is significantly more similar to one of the two independent molecules in the 2Zn insulin dimer than the other. This more similar molecule is expected to be the more stable conformer.

Animals↗
PubMed1991

Characterization of fragments of the murine Ia-associated invariant chain.

It has been proposed that invariant chain (Ii), a nonpolymorphic, transmembrane glycoprotein found in noncovalent association with Ia molecules, may function to protect the Ia Ag-binding site from association with self-peptides during Ia synthesis. Selective binding of foreign antigenic peptides could then be allowed by the dissociation of Ii molecules from Ia in the appropriate intracellular compartment. In this study, we have examined the structure and intracellular trafficking patterns of a putative proteolytic product of Ii, p25. We found that p25 is a non-membrane-bound fragment of Ii with an N terminus beginning at Met98 of the Ii sequence. p25 is formed at a very early stage of Ii synthesis in the rough endoplasmic reticulum rather than in a post-Golgi Ag-processing compartment. We have also characterized a second Ii-related species, p28, which has not been reported previously. The p28 form of Ii, unlike p25, is generated under acidic conditions similar to those found during Ag processing.

Amino Acid Sequence↗
PubMed1991

Non-radioactive techniques for the labelling of nucleic acids.

Non-radioactively labelled probes potentially have several advantages over radioactively labelled ones, such as increased stability and reduced hazard. As yet, no non-radioactive methods of labelling are as robust or produce as sensitive a probe as 32P. However, there are many options, some of which use approaches familiar to 32P users (nick translation, random priming, tailing). The majority of methods, whether enzymatic or chemical, direct or indirect, ultimately require the detection of an enzyme by a colourimetric or chemiluminescent substrate. Such detection can be achieved variously by direct visualisation (e.g. of a colourimetrically stained blot), by film, by microtitre plate reader or by CCD camera, depending on the choice of assay format and the degree of quantification required.

Journal Article↗
PubMed1990

Boron neutron capture enhancement of 252Cf brachytherapy.

Dosimetric and radiobiological studies were undertaken to investigate the potential enhancement in dose, dose distribution and cell killing effectiveness of 252Cf brachytherapy achievable when boron-10 enriched compounds are incorporated into simulated 252Cf brain implants. Thermal neutron distributions in a human head phantom containing a 252Cf source were measured by gold foil activation and calculated using a 1-dimensional transport code. This information was then used to modify measured event size distributions for 252Cf neutrons to determine the corresponding increase in dose and dose equivalent throughout the phantom. The addition of subtoxic levels of boron-10 to a typical 252Cf implant was found to significantly enhance both the absorbed dose and the high LET event frequency at distances of 3 to 5 cm from individual sources. Some unexpected geometric considerations are discussed. Reduced survival of cultured Chinese hamster cells correlated with the predicted increase in absorbed dose from the capture events with a concentration of about 60 micrograms 10B per ml in the culture medium. It was found that boron increased alpha (the "single-hit" parameter of the linear quadratic survival model) by 32% and decreased beta (the "double-hit" parameter) by 8%. The alpha/beta ratio increased to 4.34 Gy in the presence of boron, from 3.03 Gy in its absence. This translated to an 8% reduction in californium dose needed to effect 10% cell survival. It is concluded that there is a sufficiently high thermal neutron fluence present during californium brachytherapy for boron neutron capture dose augmentation to be feasible.

Animals↗
PubMed1989

Evidence for heterogeneity of the DPA and DPB alleles derived from a DRw11,DQw7,DPw4 cell line.

We have previously reported the complete sequence of the cDNAs corresponding to the alleles at the polymorphic loci (DRB1, DRB3, DQA1, DQB1) of the DRw11(5) cell line Swei. We now report the nucleotide and derived amino acid sequence of the alleles at the remaining two polymorphic loci, DPA1 and DPB1, from Swei, which types as DPw4b. Comparison of the DPw4b alpha sequence with previous DP alpha sequences indicates that it is identical to the DP alpha chains of DPw4 and DPw2. However, the DPw4b alpha cDNA encodes a unique 3' untranslated region that corresponds to sequences contained within the last intron of the DP alpha genomic gene. These results indicate that alternative splicing occurs within the Swei DP alpha RNA. Comparison of the DPw4b beta sequence with previously reported DP beta sequences indicates that the first domain is identical to the sequence of the DP beta chain derived from the Burkhardt (Burk) cell line, but that the second domain differs by two amino acids. In addition, a single amino acid difference from DPw2 at position 69 in the first domain of the DPw4b beta chain suggests that this residue is important in determining DP specificity. Furthermore, comparison with other DP beta DNA sequences suggests that variable regions occur at positions 8-11, 55-57, and 84-87.

Amino Acid Sequence↗
PubMed1989

Pulmonary complications from nasoenteral feeding tube insertion in an intensive care unit: incidence and prevention.

We prospectively evaluated 71 nasoenteral feeding tube insertions in critically ill patients and found a 4% incidence of pulmonary complications. All pulmonary complications occurred in tracheally intubated patients. To prevent these complications, we developed an enteral feeding tube insertion technique using a 26-Fr red rubber catheter as an introducer for a 8-Fr feeding tube. This technique is easy to perform by hospital staff and well tolerated by patients. No pulmonary complications occurred in 31 subsequent feeding tube insertions.

Adult↗
PubMed1984

On the occurrence of polymers of H1, H1(0) and H5 in extracts of whole tissues. Artificial production during protein preparation.

Inspection of preparations of H1, H1(0) and H5 histones made by extraction of whole tissues has shown that dimers and higher polymers of all three of these proteins are present. They may be formed by the cross-linking action of poly(ADP-ribose) chains which are linked to some of the protein molecules. Putative dimers and higher polymers were noted in preparations from various tissues and species. However, evidence is presented which suggests that production of these polymers is an artifact of precipitation during the preparation of the proteins, so the significance of the polymers is questionable.

Animals↗
PubMed1984

A survey of H1o-and H5-like protein structure and distribution in higher and lower eukaryotes.

A survey of H1o and H5-like proteins has been conducted through a range of higher and lower eukaryotic species. All mammals examined possessed H1o proteins, although in variable amounts, and the protein's structure was well conserved, though not invariant. The testis-specific histone Hlt (from rat) did not have an H1o-like structure and it appears that H1o does not occur in spermatocytes in any form. The results also show that Xenopus laevis contains H1o-like proteins, but lower, non-vertebrate eukaryotes (a crustacean, two fungi and a plant tissue) do not possess H1o or H5 proteins. The evidence suggests that H1o and H5 proteins may be considered as belonging to one family, distinct from H1 types. This H1o/H5 family may well be 'replacement histone' variants of H1. The results do not support suggestions of roles such as repression of DNA synthesis or of transcription for H1o/H5 proteins.

Animals↗