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Biomedical subjects

M Pruszczynski

Publications and source records attributed to M Pruszczynski.

At least 37 records · Page 2Linked to original sources
PubMed1995

Unusual intracytoplasmic inclusions in metastatic carcinoma. Discussion of their possible significance.

Unusual filamentous inclusion bodies in the cytoplasm of metastatic tumor cells are described. Their presence (intermingled with zymogen granules) seems rather restricted to cells of primary or metastatic acinar cell carcinoma of the pancreas, acinic carcinoma of salivary gland (parotid gland) and Paneth cells (neoplastic or in zinc deficiency state). For the time being, the real nature of these inclusions (deranged zymogen granules?) is unclear. This case also emphasizes the value of electron microscopy in solving the problem of the occult primary tumor and avoiding the misdiagnosis of an endocrine tumor (e.g. islet cell tumor or carcinoid), or a duct cell tumor with eosinophilic granular cytoplasm or in recognizing the foci of acinar cells in a mixed variant of carcinoma of the pancreas.

Aged↗
PubMed1995

Unilateral hearing loss due to a rhabdomyoma in a six-year-old child.

A case report of a six-year-old child is presented, who had had a unilateral sensorineural hearing loss for several years. Because of impairment in the ABR as well as in the caloric testing a MRI and CT scan were performed. A 17 mm tumour in the cerebellopontine angle (CPA) was detected, which after suboccipital surgery proved to be a rhabdomyoma. This tumour has not been described before in the CPA. Unilateral sensorineural hearing loss should, at all ages, be an indication for further (radiodiagnostic) investigations.

Cerebellar Neoplasms↗
PubMed1995

Aneurysmal bone cyst. Long-term results and functional evaluation.

A study was performed on 13 patients with 16 primary aneurysmal bone cysts (ABC) to assess long-term results and functional outcome. The average age at diagnosis was 19.8 years. The anatomical sites were femur (4), tibia (5), humerus (2), fibula (2) and other (3). Four ABC's required no treatment. One patient underwent radiotherapy for an ABC of the spine. Eleven ABC's were treated surgically, mostly by intracapsular resection and bone grafting. Three patients had a total of 6 recurrences, which needed further treatment. At the time of follow-up (average period: 102 months), the average functional evaluation rate of 13 patients was 96%. These functional results were graded as excellent in 9 patients and good in 4.

Adolescent↗
PubMed1994

Chondrosarcoma of bone: oncologic and functional results.

A retrospective review of 27 patients (21 males and 6 females) with chondrosarcoma of bone was performed to evaluate the oncologic and functional results. The average age of the patients was 48 years (range: 17-76). The tumor sites were pelvis in 10 cases, distal femur in 2, proximal tibia in 3, rib in 2, and other in 10. One patient with Ollier's disease had chondrosarcoma at two different sites simultaneously. Surgical stages were I A in 8 cases, I B in 8, II A in 1, and II B in 10. Two patients had no surgical treatment. Amputation was performed in 5 cases and en bloc resection in 20. An uncontaminated radical or wide surgical margin was achieved in 13 of 25 cases. Additional surgery was performed in seven cases; for deep infection in one case and for local recurrence or metastasis in six. Local recurrence developed in seven patients (26%). At follow-up, four patients had died of disease (15%), one patient had died of another cause, and two patients were alive with disease (7%). No evidence of disease was found in 20 patients (74%). The oncologic results of limb salvage and amputation in chondrosarcoma of the limbs were good, although there was one failure of limb salvage, caused by infection. Due to surgical inaccessibility, results of the management of chondrosarcoma of the pelvis were poor. The average functional evaluation of 16 patients (average follow-up: 104 months) was 75% (range: 30-100). The Musculoskeletal Tumor Society ratings were excellent in four cases, good in seven, fair in four, and poor in one.

Adolescent↗
PubMed1994

Giant cell tumor of bone: oncologic and functional results.

Giant cell tumor of bone is a challenging surgical problem due to its mostly aggressive growth with tendency to recur locally, to develop in rare instances pulmonary metastases without histologic evidence of malignant changes, and due to its potential to dedifferentiate into a frankly malignant tumor in a limited number of patients. It is treated in many different ways because of the difficulties in finding a type of treatment with the best functional results without compromising oncologic results. This paper describes 19 patients with giant cell tumor of bone. Following 19 procedures (including 6 intracapsular resections [curettage]) in 17 patients in our hospital only 2 recurrences (10.5%) occurred, both after curettage. Functional results after curettage without recurrence were favorable. Marginal or wide resections did not result in any recurrence, but were functionally inferior to curettage; an exception to the latter was the resection-arthrodesis of the distal radius in one patient.

Adult↗
PubMed1994

Severe osteomyelitis due to the zygomycete Apophysomyces elegans.

We describe a previously healthy 69-year-old man presenting with osteomyelitis of the humerus due to the zygomycete Apophysomyces elegans. The infection was acquired in Aruba, The Netherlands Antilles. The skin provided the most likely portal of entry, although there was no history of a traumatic inoculation. The patient had no history of diabetes, and no underlying immune defects were found. Despite treatment with 7.9 g of amphotericin B, an interthoracoscapular amputation proved necessary to curtail the rapid spread of the fungus in this immunocompetent host.

Aged↗
PubMed1992

Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia.

Expression of keratins 5, 14 and 17 in endocervical subcolumnar reserve cells was detected by means of immunohistochemical studies using polypeptide specific monoclonal antibodies. These particular keratins that were found among others in basal cells could also be detected to a variable extent in metaplastic and dysplastic cervical lesions. In some cases of immature squamous metaplasia all three keratin subtypes were expressed throughout the full thickness of the epithelium. In contrast, in mature squamous metaplasia a compartmentalization of these keratins was observed. Mature squamous metaplastic epithelium showed a keratin distribution pattern comparable to ectocervical squamous epithelium, with the exception of keratin 17, which was only sporadically found in the basal layer of ectocervical epithelium and was always present in the basal cells of mature squamous metaplastic epithelium. During progression of cervical intraepithelial neoplasia a clear increase in the expression of keratin 17 was observed. However, also keratins 5 and 14 were expressed. Our results demonstrate that a considerable number of premalignant lesions of the uterine cervix express the same keratins as found in the progenitor reserve cells. Lesions that lack expression of keratin 17 may form a distinct group, which are regressive in nature and do not progress into cervical cancer.

Cervix Uteri↗
PubMed1992

Keratin expression in cervical cancer.

Using a panel of 21 monoclonal and 2 polyclonal keratin antibodies, capable of detecting separately 11 subtypes of their epithelial intermediate filament proteins at the single cell level, we investigated keratin expression in 16 squamous cell carcinomas, 9 adenocarcinomas, and 3 adenosquamous carcinomas of the human uterine cervix. The keratin phenotype of the keratinizing squamous cell carcinoma was found to be most complex comprising keratins 4, 5, 6, 8, 13, 14, 16, 17, 18, 19, and usually keratin 10. The nonkeratinizing variety of the squamous cell carcinoma expressed keratins 6, 14, 17, and 19 in all cases, usually 4, 5, 7, 8, and 18, and sometimes keratins 10, 13, and 16. Adenocarcinomas displayed a less complex keratin expression pattern comprising keratins 7, 8, 17, 18, and 19, while keratin 14 was often present and keratins 4, 5, 10 and 13 were sporadically found in individual cells in a few cases. These keratin phenotypes may be useful in differential diagnostic considerations when distinguishing between keratinizing and nonkeratinizing carcinomas (using keratin 10, 13, and 16 antibodies), and also in the distinction between nonkeratinizing carcinomas and poorly differentiated adenocarcinomas, which do not express keratins 5 and 6. Keratin 17 may also be useful in distinguishing carcinomas of the cervix from those of the colon and also from mesotheliomas. Furthermore the presence of keratin 17 in a CIN I, II, or III lesion may indicate progressive potential while its absence could be indicative of a regressive behavior. Because most carcinomas express keratins 8, 14, 17, 18, and 19, we propose that this expression pattern reflects the origin of cervical cancer from a common progenitor cell, i.e., the endocervical reserve cell that has been shown to express keratins 5, 8, 14, 17, 18, and 19.

Adenocarcinoma↗
PubMed1991

The midline cervical cleft.

The midline cervical cleft is a rare congenital anomaly of the ventral neck. With reference to two patients with a midline cervical cleft, we discuss the clinical picture, the embryology, and the surgical treatment.

Female↗
PubMed1990

Atypical mycobacterial infection of the parotid gland.

A localized atypical mycobacterial infection of the major salivary gland is a rare disease. In this report the cases of three patients with this lesion are presented. The diagnosis was based on the clinical picture, skin testing with specific antigens, bacteriologic culture, and histopathologic findings. The patients were successfully treated by total parotidectomy with facial nerve preservation, which in our opinion is the therapy of choice in localized atypical mycobacterial infections.

Child, Preschool↗
PubMed1990

Cytokeratin expression in chondroblastomas.

The present study is based on the histopathological and immunohistochemical examination of seven chondroblastomas, including one lung metastasis occurring 9 years after treatment. Chondroblastomas were shown to co-express vimentin, S-100 protein, neuron-specific enolase, and the epithelial markers recognized by CAM 5.2, EMA and a polyclonal cytokeratin antibody. The cytokeratins present in the tumour cells of the lung metastasis were characterized as cytokeratins 8, 18, 19 and, to a lesser extent, cytokeratin 7. The results suggest aberrant cytokeratin expression in chrondroblastomas.

Adolescent↗
PubMed1990

Late pulmonary metastases from chondroblastoma of the distal femur. A case report.

A patient treated with curettage and bone grafting for a chondroblastoma of the distal femur sustained a pathologic fracture after a local recurrence. He then underwent a local, radical resection and an arthrodesis of the knee. Ten years after the first operation, pulmonary metastases were found, for which he underwent a metastasectomy (thoracotomy). At the latest follow-up, 1 year later there were no signs of tumor.

Adult↗
PubMed1990

Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia.

The expression of keratins in normal cervical epithelia, metaplastic epithelium, and cervical intraepithelial neoplasia (CIN) grades I, II, and III is investigated with a panel of keratin polypeptide-specific monoclonal antibodies. This approach allowed the detection of individual keratins 4, 7, 8, 10, 13, 14, 18, and 19 at the single-cell level. By using an antibody recognizing keratins 5 and 8 (RCK 102) and two antibodies specific for keratin 8 (CAM 5.2 and M 20), it was also possible to derive information on the distribution of keratin 5. Our results show that during immature squamous metaplasia there is an acquisition of keratins typical of squamous epithelium, ie, keratins 4, 5, 13, and 14. This process continues during further differentiation to mature squamous metaplasia. In premalignant lesions the expression pattern of the progenitor reserve cells and immature squamous metaplastic epithelium is partly conserved. However, in most cases an induction in the expression of the keratins 4, 13, and 14 was observed. Furthermore, CIN III shows a more extensive expression of keratins typical of simple epithelia, ie, keratins 8 and 18, as compared to CIN I and CIN II.

Adult↗
PubMed1989

Causes of late complications in children operated on for Hirschsprung's disease: a preliminary immunohistochemical investigation using polyclonal antibodies against S-100 protein.

Late follow-up of 51 children operated on for the classical form of Hirschsprung's disease showed a great many unsatisfactory results. It is well known from the literature that achalasia of the anal sphincter is one of the most important causes. Twenty-five patients underwent redilatation of the anal sphincter. Complaints in the form of obstipation or diarrhoea remained in 40% of the cases after redilatation. Reinvestigation of the resected colon specimens using the conventional histological method showed the presence of ganglion cells in the proximal resection fragment in all patients. In the light of reports in the literature stating that despite normal histology, innervation disturbances can exist in the colon, we conducted a pilot study using polyclonal antibodies against S-100 protein. In three out of four patients with an adequate resection, in accordance with the conventional histological criteria, clear innervation abnormalities could be seen with the aid of polyclonal antibody staining.

Child↗
PubMed1988

Immunohistochemical and ultrastructural studies of a primary aortic intimal sarcoma.

An intimal sarcoma of the abdominal aorta in a 63-year-old woman is reported. The clinical symptoms consisted of chronic arterial hypertension, vomiting and epigastric pain. Treatment was operative, but the patient died 20 hours after surgery. The studies were performed on a surgical specimen and on autopsy material. The aortic tumour consisted of pleomorphic spindle-shaped and giant cells. In the vertebral metastases a storiform pattern of the tumour cells was found. No specific features characteristic for leiomyogenic, lipogenic or an endothelial nature of the tumour giant cells was disclosed in electron microscopy and the picture rather indicated their histiocytic character. Of the 18 cellular markers studied, the immunostainings for vimentin and alpha-1-antichymotrypsin were evidently positive. The tumour was classified as a pleomorphic intimal aortic sarcoma probably a malignant fibrous histiocytoma (MFH). The literature on 26 previously published aortal tumours is reviewed with emphasis on their topographical distribution and histological classification. In only 4 previous cases was the final diagnosis supported by electron microscopical or immunopathological findings. The role of marker studies in the classification of aortal tumours is discussed.

Aorta, Abdominal↗