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Biomedical subjects

M Otani

Publications and source records attributed to M Otani.

At least 163 records · Page 9Linked to original sources
PubMed1977

Immunological stability in vitro and in vivo of eel calcitonin.

The immunological activity of eel calcitonin was more persistent than that of porcine and human calcitonin when incubated in vitro with rabbit serum, human serum, liver homogenate or kidney homogenate, respectively. Both eel and human calcitonin were more stable than porcine calcitonin when injected iv into rabbits.

Animals↗
PubMed1977

Stability of [Asu1,7]-eel calcitonin and eel calcitonin in vitro and in vivo.

Stability of [Asu1,7]-eel calcitonin was compared with that of eel calcitonin by the radioimmunoassay method. [Asu1,7]-Eel calcitonin cross-reacted with anti-eel calcitonin antisera. [Asu1,7]-Eel calcitonin was as stable as eel calcitonin in rabbit liver or rabbit kidney extracts when incubated at 37 degrees C. Disappearance curves from the plasma of [Asu1,7]-eel calcitonin and eel calcitonin following iv injection in rabbits were quite similar.

Animals↗
PubMed1976

Isolation and characterization of calcitonin from pericardium and esophagus of eel.

Calcitonin was extracted from the pericardium and esophagus of eel in quantities sufficient to permit purification and chemical characterization. Homogeneous calcitonin could be isolated by a six-step fractionation starting from acetone powder of the organs. The fractionation procedure consisted of acid extraction, gel filtration on Sephadex G-75, chromatography on SP-Sephadex C-25, gel filtration on the Sephadex G-50, chromatography on carboxymethylcellulose, and gel filtration on Sephadex G-50. Fractionation of the hormone was monitored by assay of its biological activity and from its behaviour on thin layer chromatography and polyacrylamide gel disc electrophoresis. The hormone contained 32 amino acid residues, like calcitonins from other species of animals, but its amino acid composition was different from those of previously characterized hormones. Eel calcitonin possessed almost the same, or higher, biological activity as the salmon or chicken hormone, which show the highest specific activity among calcitonins so far isolated.

Amino Acids↗