Biomedical subjects

M Oshima

Publications and source records attributed to M Oshima.

363 records · Page 21Linked to original sources

PubMedDate not supplied

Implications of medical information systems for community health care in Japan.

The population of Japan in the 21st century is expected to be distributed heavily on the side of higher ages. In view of the aged society, improved hygienic, medical, and welfare services must be provided in every community. In order to provide these services efficiently it is necessary to implement a community health care system (CHCS). The role and responsibilities of the medical information system, which is intended to support comprehensively the CHCS with respect to providing the necessary information, will become increasingly important. This paper is intended to discuss the structure, features, and required activities of the community medical information system which will have particular importance in the community health care services in the coming years. Problems in establishing and operating future medical information systems in the CHCS also are discussed. Lastly, problems and obstacles confronted when establishing and operating medical information systems in community health care which is organized around the medical association, which plays an important role and takes on heavy responsibility, are discussed, and a proposal is made.

Ambulatory Care Information Systems↗

PubMedDate not supplied

Reactivity of a mouse/human chimeric anti-GM2 antibody KM966 with brain tumors.

With the aim of investigating the passive immunotherapy of brain tumors, we examined the binding of a mouse/human chimeric anti-ganglioside GM2 antibody KM966 to various organs and brain tumors. Frozen sections of 51 surgically resected brain tumors were stained with antibody KM966. Fourteen gliomas out of 16 were stained positively with antibody KM966. Eleven positive sections demonstrated homogenous staining. No specific binding to normal gray matter and white matter was observed. Some cases of meningiomas, neurinomas and metastatic brain tumors were stained with KM966 but with less frequency and intensity than gliomas. In addition, KM966 demonstrated strong antibody-dependent cellular cytotoxicity against human malignant glioma cells. These results showed that antibody KM966 will be useful for passive immunotherapy of malignant gliomas.

Adult↗

PubMedDate not supplied

Different binding properties of three monoclonal antibodies to sialyl Le(x) glycolipids in a gastric cancer cell line and normal stomach tissue.

We established a mouse monoclonal antibody (Mab) KM93 which recognized sialyl Le(x)-carbohydrate epitope determined by solid phase radioimmunoassay using a panel of authentic glycolipids. The specificity of KM93 was similar to another anti-sialyl Le(x) Mab CSLEX-1 established previously, and different from that of Mab FH6 which recognized sialyl Le(x)-i (sialyl dimeric Le(x)). In a further study, however, we found that KM93 reacted with some glycolipids much more strongly than CSLEX-1 did on thin-layer chromatography (TLC) plates. We purified two gangliosides named K-1 and K-2 from gastric cancer cell line KATOIII, and three gangliosides named H-1, H-2, and H-3 from human stomach. KM93 reacted with all of these glycolipids. CSLEX-1 reacted with K-1 and K-2 with less intensity than KM93 did, and faintly reacted with H-1, but not at all with H-2 or H-3. FH6 did not react with K-1, K-2 or H-1, while it stained H-2 and H-3. In spite of this different reactivity with Mabs, analysis by proton nuclear magnetic resonance (1H NMR) proved that carbohydrate structure of K-1 and H-2 were the same: NeuAc alpha 2-->3Ga1 beta 1-->4 [Fuc alpha 1-->3] G1cNAc beta 1-->3 Ga1 beta 1-->4G1c beta 1-->1Cer. The H1 NMR spectrum of H-3 also indicated that H-3 consisted of the same sugars as K-1. These results indicated that KM93 had wider reactivity than CSLEX-1, and that the distinct reactivity of KM93 from CSLEX-1 was not caused by sugar moiety. It was also shown that the interaction of sialyl Le(x) sugar determinant with MAb depended on the tissue origin of molecules carrying carbohydrate.

Amino Acid Sequence↗