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Biomedical subjects

M Ohata

Publications and source records attributed to M Ohata.

At least 91 records · Page 5Linked to original sources
PubMed1991

[A case of primary intrapulmonary benign schwannoma].

A case of primary intrapulmonary schwannoma was described. A 72-year-old man was admitted to our hospital because of dry cough. A chest radiograph showed partial atelectasis of the middle lobe, but computed tomogram of the chest revealed no tumor shadow. Bronchoscopy disclosed a pale-yellow uneven endobronchial wall of the right middle lobe bronchus. Transbronchial biopsy revealed benign schwannoma. Primary intrapulmonary schwannoma has been rarely reported.

Aged↗
PubMed1990

[Establishment and characterization of human cancer cell line (NOC-S) derived from a progressive lung cancer].

The results of the transplantation out of a progressive lung cancer tissue with leukocytosis showed that tumors were grown in nude mice at about 70 days after direct transplantation. A tumor was removed from the nude mouse for attempt at establishment of cell line in vitro. The medium used for the cell culture was MEM synthetic culture medium (Gibco Inc.) supplemented with 10% new borne bovine serum. Subcultures were performed on 5 to 6 days basis at 1:2 split by the use of 0.25% trypsin solution. The morphology of obtained cells was a spindle-like shape and the cell grew in a monolayered sheet with about 32 to 36 hr of population-doubling time. The modal chromosome number of this cell line was 44 including one large submetacentric and one minute marker chromosome. Cell lines were tested for tumorigenicity with an inoculum of five million cells in nude mice. This cell lines produced tumors with high rate. The tumor has been maintained in nude mice through serial passages over a period of one year. The temperature sensitivity and the colony stimulating factor-producing of this cells will be discussed.

Adenocarcinoma↗
PubMed1990

[A case of thymolipoma on the cervicomediastinal area].

Thymolipoma is a very rare mediastinal tumor. We reported a case of 52-year-old female with thymolipoma which was located in the cervicomediastinal area. The chest X-ray film revealed an abnormal shadow in the superior mediastinum. Computed Tomography (CT) clearly showed the existence of a large mass in the left side of the trachea. The angiogram showed that there was a stenosis on the left brachiocephalic vein. On June 13, 1988, median sternotomy was performed. A large tumor, about 5.5 x 13 x 5.5 cm, was found arising from the left cervical area. This tumor was excised completely and thymolipoma was diagnosed histopathologically. The post-operative course was very satisfactory.

Female↗
PubMed1990

[Serum adenosine deaminase and its isozyme activity in leukemia and MDS].

We studied the activity of serum adenosine deaminase (ADA) and its isozyme in 36 leukemic patients (16 ANLL, 11 ALL, and 9 CML) and 8 MDS. Isozyme was measured by erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) inhibitory assay. This assay was simple and reliable. The appearance rate of abnormally high ADA value were 81.24% for ANLL, 100% for ALL, 77.8% for CML and 37.5% for MDS. The ADA level became high when MDS turned into overt leukemia. In isozyme pattern, there was a clear difference between ANLL and ALL. The isozyme I/II ratio was significantly higher (p less than 0.001) in ALL than ANLL. Lymphoblastic crisis of CML also had a high isozyme I/II ratio. There was a correlation between isozyme I and absolute number of peripheral blasts in ALL (r = 0.768). When observed time sequentially, ADA and isozyme changed correlatively with the number of blasts counts. Serum ADA and its isozyme are useful parameters both for leukemic diagnosis and treatment.

Adenosine Deaminase↗
PubMed1990

[Combination assay of IAP and ADA in hematologic malignancies].

We measured the levels of adenosine deaminase (ADA) and immunosuppressive acid protein (IAP) in 10 patients with acute myeloid leukemia (AML), 5 with acute lymphoblastic leukemia (ALL), 8 with chronic myeloid leukemia (CML), 7 with myelodysplastic syndrome (MDS), 5 with malignant lymphoma (ML), 3 with multiple myeloma (MM) and one with adult T cell leukemia. On admission, the level of IAP was abnormally high in all cases of AML and ALL 50% of CML cases, 71.4% of MDS cases, 60% of ML cases and none of MM cases. ADA was elevated in all cases of ALL, 77.8% of AML and CML cases, 57.1% of MDS cases, 60% of ML cases and 33.3% of MM cases. In 7 patients with AML, the level of IAP returned to normal when they achieved complete remission. On the other hand, the level of ADA had already returned to normal even during induction therapy. ADA showed a positive correlation with the absolute number of peripheral blasts and lactic dehydrogenase both in AML and ALL. These results suggest that ADA indicates the activity of leukemia and IAP indicates the immunocompetence of the host.

Adenosine Deaminase↗
PubMed1989

[Changes in urinary prostaglandins during cardiopulmonary bypass in man].

We measured urinary and plasma levels of thromboxane B2 (TXB2), 6-keto PGF1 alpha and urinary NAG during cardiopulmonary bypass (CPB) and studied their clinical significance. Subjects studied were 10 patients undergoing coronary artery bypass graft. Urinary and plasma levels of TXB2 and 6-keto PGF1 alpha increased during CPB. However, predictive plasma levels of TXB2 and 6-keto PGF1 alpha, calculated by urinary excretion, were higher than actual plasma levels. This result suggests that the kidney produces TXA2 and PGI2. Significant correlation (r = 0.87, P less than 0.01) was observed between NAG and TXB2 during CPB. The results suggest that TXA2 impairs the function of the renal urinary tubules.

Acetylglucosaminidase↗
PubMed1989

[Surgical treatment of bilateral spontaneous pneumothorax].

88 cases of bacterial spontaneous pneumothorax, 20 cases, occurred same time and 68 cases, occurred different time, were treated in our Department of Thoracic Surgery from 1975 to July 1987, and represented 16.6% of all cases of spontaneous pneumothorax. The characteristics of bilateral spontaneous pneumothorax, occurred same time, were as follows; 1) frequent in teens, 2) often occurred with history of unilateral spontaneous pneumothorax, 3) slightly collapsed. For the treatment of bilateral spontaneous pneumothorax, occurred same time, bilateral thoracotomy was necessary. The characteristics of bilateral spontaneous pneumothorax, occurred different time, were as follows; 1) frequent in the twenties, 2) often occurred the other side after unilateral thoracotomy within a year. For the treatment of bilateral spontaneous pneumothorax, occurred different time, if the patient is under 20 thoracotomy must be carried out, but if the patient is over 20 at the side of pneumothorax must be operated.

Adolescent↗
PubMed1989

[In vivo protective action of urinastatin during extracorporeal circulation].

A patient undergoing extracorporeal circulation is in the state of controlled shock. The object of the present study is to investigate the prophylactic effect of ulinastatin (6000 U.kg-1 or 12000 U.kg-1 during extracorporeal circulation by a randomized study. Although the ratio of lactic acid/pyruvic acid did not show significant changes, the increase was inhibited in 12000 U.kg-1 treated group. In 12000 U.kg-1 treated group, beta-glucuronidase reflecting the function of lysosomal membrane, as well fibronectin reflecting the endothelial function, were suppressed. The results of the present study indicate that administration of ulinastatin 12000 U.kg-1 is effective and useful for the organism.

Extracorporeal Circulation↗
PubMed1988

Effects of propranolol and nitroglycerin on cephalad collateral venous flow in patients with cirrhosis: evaluation using transesophageal real-time two-dimensional Doppler echography.

We used transesophageal real-time two-dimensional Doppler echography (TE2DD) to assess the effects of propranolol (n = 18, 6 mg each) and nitroglycerin (n = 18, 0.5 mg each) on blood flow in the intercostal veins, azygos vein, thoracic aorta, and esophagogastric varices. The primary disease in all of the patients was liver cirrhosis. Propranolol infusion markedly reduced the flow velocity in the varices, intercostal vein, azygos vein, and thoracic aorta (-24%, -41%, -34%, and -24%, respectively). It also significantly reduced the blood flow volume index (BFVI), defined as mean velocity in cm/sec X the square of the diameter in cm2 of both the azygos vein and the aorta (-34%, -21%, respectively). Nitroglycerin infusion did not cause significant changes in the hemodynamics of the above vessels, because the hemodynamic responses to the drug differed from individual to individual. The BFVI of the azygos vein correlated well with the azygos venous flow measured by the conventional thermodilution technique (r = 0.79, p less than 0.01). TE2DD appears to be a useful method for studying the hemodynamics of ascending collaterals in patients with portal hypertension.

Aged↗
PubMed1987

A transmission electron microscopic study of rabbit liver sinusoids with special remarks on an experimentally induced canalicular system and the "pored domes" in the endothelial cells.

Rabbits were starved for a week and then allowed to take the standard diet; the animals were sacrificed after 20 or 24 hr. One group of the animals were treated in winter, and the other, in summer. Control animals were fed solid rabbit food ad libitum. In the control rabbits, short rudimentary canaliculi and occasional small-sized pored domes were found in the thicker portions of the endothelial extension. In the animals experimentally treated in winter, well-developed meandering canaliculi with vacuolar expansions and constrictions appeared in the thicker portions and perikaryonal cytoplasm. In addition prominent pored domes were produced. The canaliculi often formed sponge-like networks with openings both to the sinusoid and the Disse's space. The development of the canalicular system and pored domes correlated to an increased blood flow through the sinusoids; this seemed to be induced upon sudden feedings after lengthy starvation. The rabbits subjected to the experimental treatment in summer exhibited no distinct development of the canaliculi and pored domes. This result was ascribed to the inhibited functions of the rabbit liver during this season.

Animals↗
PubMed1986

Experimental study on the fine structure of chicken liver parenchyme with special references to extrasinusoidal macrophages and sinusoidal blood cells. Part 2. Sinusoidal blood cells in normal and India ink perfused livers.

Leucocytes and thrombocytes in the chicken liver sinusoids were observed under normal conditions and after intravenous India ink perfusion. The monocytes exhibited conspicuous phagocytic activity. At 30 min or earlier and 4 hr after the perfusion, they ingested considerable amounts of the carbon particles, which were deposited in small vacuoles and/or lysosomes. In this study we revealed two transitional forms of the monocyte changing into the Kupffer cell. In one transitional form, which already at 15 min after the perfusion stored considerable amounts of the particles, the ectoplasmic layer was partly differentiated and projected many pseudopodia into the sinusoid. At 48 hr after the perfusion, the other transitional form was attached by its wide basal surface to the endothelial linig and projected well-developed pseudopodia into the sinusoid like the Kupffer cell without, however, storing the carbon particles. These findings are thought to suggest the transformation of the monocytes into the Kupffer cells. Thus we came to the assumption that the Kupffer cells might be replenished: by self-proliferation; by the macrophages from the hepatic parenchyme into the sinusoid; or by transformation from the monocytes circulating into the sinusoid (the "triple origin" as opposed to the "dual origin" of the Kupffer cell). In the earliest stage after India ink perfusion, the thrombocytes exhibited the most striking reaction comparable to the Kupffer cells toward which they were assembled. The India ink particles were taken up into the "surface connected canalicular system" (SCS), which thickened and made vacuolar expansions as the amount of the particles was increased. At 4 hr after perfusion, the particles disappeared from the majority of the thrombocytes, leaving an empty SCS. The India ink particle uptake and storage by the thrombocyte were thought to be temporary phenomena, different from the true phagocytosis of the macrophages.

Animals↗
PubMed1986

Experimental study on the fine structure of chicken liver parenchyme with special references to extrasinusoidal macrophages and sinusoidal blood cells. Part 1. Sinusoidal cells and macrophages in the normal and India ink-perfused livers.

The chicken livers were electron microscopically observed under a normal condition and after an intravenous India ink perfusion. Kupffer cells and sinusoidal endothelial cells commenced to endocytose India ink particles in the earliest stages (15 min) after perfusion. The attachment of the particles to the cell surface occurred only in the Kupffer cells, which actively took up the particles with coated caveolae. In the endothelial cells the particles were ingested by pinocytosis in the perikaryon and deposited in the macropinocytic vacuoles of Wisse. In Kupffer and endothelial cells, the particles were stored most abundantly at 30 min and 4 hr after perfusion, respectively. At 48 hr, the vacuoles containing the particles were decreased in number and size, while mitotic figures were revealed in the Kupffer cells. Ito cells occasionally ingested in later stages (4 and 48 hr) a few carbon particles; they underwent no mitotic division. Extrasinusoidal macrophages scattered in the parenchyme and phagocytic reticular cells (macrophages) in the lymphoid tissues exhibited phagocytic activity only in later stages (1-4 hr). In 48 hr after the perfusion both cells began to store amounts of the particles in their vacuoles. This delayed phagocytic activity may be ascribed to the location of the cells separated from the sinusoid by the endothelium. Some solitary macrophages projecting a long process into the sinusoid took up the particles at earlier stages. At 48 hr, several mitotic divisions took place in the phagocytic reticular cells of the lymphoid tissue, while no mitotic divisions were found in the solitary macrophages in the parenchyme. After the ink perfusion, migration of solitary macrophages into the sinusoid was accelerated and images indicating their transformation into Kupffer cells were frequently detected. It was concluded that the Kupffer cells maintain their necessary number not only by their self-proliferation but also by replenishment from the extrasinusoidal solitary macrophages scattered in the hepatic parenchyme, which in turn are replenished from the phagocytic reticular cells in the lymphoid tissue capable of mitotic proliferation.

Animals↗
PubMed1985

[3H]Methotrexate loss from the rat brain following enhanced uptake by osmotic opening of the blood-brain barrier.

Right brain regions of anesthetized rats were loaded with [3,5,7-3H]methotrexate ([3H]MTX) or with [14C]sucrose by infusing the tracers into the right carotid artery, after the blood-brain barrier had been opened by right carotid infusion of a hypertonic arabinose solution. During the 6 hr following the procedure, the [3H]MTX concentration in 7 right-sided brain regions, when normalized to the plasma concentration integral during tracer infusion, fell, with an average half-time of 4.8 hr as compared to less than 20 min for the initial rate of loss [14C]sucrose. Right-left brain concentration differences 3 hr after treatment were statistically significant (p less than 0.05) for [3H]MTX but not for [14C]sucrose. The results indicate that intracerebral [3H]MTX is lost more slowly than is intracerebral [14C]sucrose, possibly because [3H]MTX enters brain cells, whereas [14C]sucrose remains largely extracellular.

Animals↗