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Biomedical subjects

M Nitta

Publications and source records attributed to M Nitta.

At least 145 records · Page 8Linked to original sources
PubMed1986

Variable expression of the translocated c-abl oncogene in Philadelphia-chromosome-positive B-lymphoid cell lines from chronic myelogenous leukemia patients.

The consistent cytogenetic translocation of chronic myelogenous leukemia (the Philadelphia chromosome, Ph1) has been observed in cells of multiple hematopoietic lineages. This translocation creates a chimeric gene composed of breakpoint-cluster-region (bcr) sequences from chromosome 22 fused to a portion of the abl oncogene on chromosome 9. The resulting gene product (P210c-abl) resembles the transforming protein of the Abelson murine leukemia virus in its structure and tyrosine kinase activity. P210c-abl is expressed in Ph1-positive cell lines of myeloid lineage and in clinical specimens with myeloid predominance. We show here that Epstein-Barr virus-transformed B-lymphocyte lines that retain Ph1 can express P210c-abl. The level of expression in these B-cell lines is generally lower and more variable than that observed for myeloid lines. Protein expression is not related to amplification of the abl gene but to variation in the level of bcr-abl mRNA produced from a single Ph1 template.

B-Lymphocytes↗
PubMed1986

[Combined treatment of 8 MHz radiofrequency hyperthermia and irradiation in a patient with mediastinal metastases of renal cancer].

A combined therapy of irradiation and 8 MHz radiofrequency hyperthermia using the Thermotron-RF Model 8 was performed on a patient with mediastinal metastasis of renal cancer. The patient was a 74-year-old male, who received left transperitoneal nephrectomy for left renal tumor in December, 1982. Histology report indicated clear cell carcinoma of the kidney. He noticed puffy face and dyspnea in April, 1984. A CT scan showed a mediastinal tumor 57 X 43 X 120 mm in size and right pleural effusion. Aspiration biopsies revealed metastatic adenocarcinoma of the mediastinal lymph nodes. The combined therapy of irradiation and 8 MHz radiofrequency hyperthermia was started in July, 1984. He was irradiated with daily 2.0 Gy, 5 times a week and was heated twice a week within one hour after each irradiation, totally 50 Gy of irradiation and 14 sessions of hyperthermia. After the treatment, 69% tumor regression and disappearance of pleural effusion were obtained. The combined therapy with 28.8 Gy of irradiation and 7 sessions of hyperthermia was added for the regrowth of the tumor in February, 1985. A 30% of tumor regression was achieved, however, there was no improvement of the dyspnea or pleural effusion. He died on April 8, 1985.

Adenocarcinoma↗
PubMed1985

Colloid cysts of the third ventricle. A review of 36 cases.

Thirty-six colloid cysts were treated from 1949 to 1983. There were 26 male and 10 female patients, ranging in age from 12 to 65 years old, 60% between 31 and 40 years. Headache or disturbed mental function was the most frequent complaint, papilloedema the most frequent sign. The patients were classified into 3 groups by symptoms and signs; Group I (17 patients): Headache, papilloedema and no neurological signs. Group II (6 patients): Fluctuating or progressive dementia. Group III: Twelve cases with "classical" features, episodic headache and drop attacks. One patient could not be classified in any of these groups. Seventeen of 36 patients were diagnosed by ventriculography, 19 patients were diagnosed by CT scan. CT scan was the most reliable diagnostic study, but was unavailable in the earlier part of the series. All patients have been operated by transventricular exposure of the right foramen of Munro with incision of the middle frontal gyrus in an antero-posterior linear manner. Twenty-five of 36 patients showed an excellent operative result, and nine had a good result, one of the 9 patients dying of an unrelated intracerebral haemorrhage 4 years after operation. Two patients had a poor result, characterized by memory loss and confusion. One of these died of an unknown cause 5 years after operation.

Adolescent↗
PubMed1985

Incidence of involvement of the B and T lymphocyte lineages in chronic myelogenous leukemia.

Peripheral blood specimens were obtained from 22 patients with Philadelphia chromosome (Ph1) positive chronic myelogenous leukemia (CML) (16 in chronic phase, 2 in an accelerated phase, and 4 in blast crisis). Studies were performed to determine the frequency of the presence of the Ph1 chromosome in cells of lymphoid lineages. Rosetted (E+) lymphocytes (T lymphocytes) from nine patients in chronic phase and one patient in blast crisis were stimulated with T cell growth factor interleukin 2 (IL-2) and/or phytohemagglutinin (PHA). All ten patients had sufficient T lymphocyte metaphases for analysis and of a total of 461 metaphases examined, only one contained the Ph1 chromosome. Nucleated cells of density less than 1.077 g/mL were infected with Epstein-Barr virus (EBV). Following infection, cell lines were established from individual colonies attached to egg albumin-coated Lab-Tek slide chambers (clonal cell lines) or from suspension culture in 96-well tissue culture cluster dishes (nonclonal cell lines). Cell surface and intracellular marker analysis confirmed the B lymphocyte phenotype of all the cell lines examined. B lymphoblastoid cell lines were established from 16 of the 22 patients. All lines from 12 patients were Ph1-negative. From two chronic phase patients, both Ph1-positive and Ph1-negative lines were established. From one patient in an accelerated phase, only Ph1-positive lines were established. From another patient in blast crisis (of myeloblastic phenotype), only Ph1-positive lines were established initially; however, five months later, after the patient had been treated with mitoxantrone, only Ph1-negative lines were derived from this patient. Based on these results, it appears that most B cells and mature T cells in most CML patients are Ph1-negative, but that about 25% of patients have predominantly Ph1-positive B cells or a mixture of Ph1-positive and Ph1-negative B cells that are capable of growing as established cell lines after transformation with EBV.

Adolescent↗
PubMed1985

Clinical evaluation of urapidil, a new antihypertensive drug: preliminary findings in Japan.

The pharmacokinetics, hypotensive effects, and safety of urapidil were investigated in 22 patients with essential hypertension at four coordinated centers in Japan. The drug was administered in sustained-release capsules given twice daily. The peak plasma concentration of the drug (measured in six patients) was observed four to six hours after administration, and 60% to 70% of the peak concentration was detectable at eight hours. These findings suggest that blood pressure might be controlled by twice-daily administration of urapidil. In 14 outpatients, who received 40 to 80 mg/day of urapidil, systolic and mean blood pressure decreased significantly (P less than 0.05) by week 4, and systolic, diastolic, and mean blood pressure decreased significantly (P less than 0.001) by week 6. In four inpatients, treated with 60 to 120 mg/day of the drug for two weeks, there were significant decreases (P less than 0.05) in systolic, diastolic, and mean blood pressure at day 6 and significant decreases (P less than 0.01) in diastolic and mean blood pressure at day 13. Although side effects were noted in five (23%) of the 22 patients, they were transient and slight in three; in two patients, the side effects disappeared after use of the medication was discontinued. Because the study was carried out in a small number of patients who received different dosages of urapidil over a short period of time, the optimal dosage was not established.

Adult↗
PubMed1984

An experimental study of acute subarachnoid haemorrhage in baboons: changes in cerebral blood volume, blood flow, electrical activity and water content.

Subarachnoid haemorrhage following transection of the posterior artery was produced in 10 baboons. Cerebral blood volume (CBV) decreased transiently after subarachnoid haemorrhage. Two basic patterns of intracranial pressure (ICP) were observed; in one ICP returned to normal but in the other it remained elevated. In this latter group four out of five animals showed an increase in CBV above the original level. There were delays in sensory conduction (measured using somatosensory evoked potentials) bilaterally; those on the contralateral side to the bleed were correlated with ICP whereas other factors are implicated on the ipsilateral side. Initial flow reduction and restoration of cerebral blood flow were both correlated with water content.

Animals↗
PubMed1984

[Systems analysis of pressure transmission in intracranial cavity].

Systems analysis of intracranial pressure pulse waveform was carried out in 6 dogs during normal condition (normocapnia and intracranial normotension) in order to explore how to transmit the pressure pulse through the intracranial cavity. Pulses originated in the heart travel in the intracranial cavity through the vessels, transmit into the cerebral tissue and finally reach to the dural surface. It can be speculated that the epidural pressure pulse waveform might be influenced by the property of intracranial components. The intracranial cavity which consists of brain parenchyma, vessels, cerebrospinal fluid, blood, pia, arachnoid, dura and skull is considered as a kind of system. The systems analysis method was applied to it. The common carotid arterial pressure pulse wave was used as the input signal of system, and the epidural pressure pulse wave was used as the output. The intracranial pressure was measured by an epidural pressure transducer attached to the dural surface through a parietal burr hole. The common carotid arterial pressure pulse wave and the epidural pressure pulse wave were recorded on FM analog tape. The characteristics of transmission are evaluated by the exact transfer function of system. We consider that the exact transfer function of system is obtained by the randomized input signal caused by the cardiac pacemaker or the aorta balloon. The power spectrum of input signal in this method didn't have a flat profile like completely random wave (or white noise) but a gentle slope between 1 Hz and 20 Hz. Therefore, the result in our method may be reliable only on the waves between 1 Hz and 20 Hz.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗