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Biomedical subjects

M Nadji

Publications and source records attributed to M Nadji.

At least 109 records · Page 6Linked to original sources
PubMed1981

Carcinoembryonic antigen present in human eccrine sweat.

Carcinoembryonic antigen is detectable by standard unlabeled peroxidase-antiperoxidase technics in eccrine and apocrine secretory and ductal cells. Carcinoembryonic antigen is also present in the cuticle which lines the duct. In eccrine sweat, carcinoembryonic antigen activities were at least thirty times that in serum, using a radioimmunoassay. Carcinoembryonic antigen may form part of the Schiff-positive cuticle and function in the normal homeostasis of eccrine and apocrine glands.

Apocrine Glands↗
PubMed1981

Early electrophysiologic and anatomic alterations in cat ventricular muscle after coronary artery ligation.

The effect of coronary artery ligation on electrophysiologic properties of cat ventricular muscle cells was studied. Depression of resting potential, action potential rate of rise and amplitude was observed in infarcted cells, 30 min to 5 days after ligation. Action potential duration was markedly shortened in acute stages (30-120 min) but gradually lengthened to above control by 48 h. Anatomic sequelae included oedema, loss of fibre striation and cellular necrosis.

Action Potentials↗
PubMed1981

Kaposi's sarcoma: immunohistologic evidence for an endothelial origin.

Factor VIII-related antigen (VIIIR-Ag), a marker for endothelial cells, was used to explore the histogenesis of Kaposi's sarcoma. Formaldehyde-fixed, paraffin-embedded routine histologic sections from 37 cases of Kaposi's sarcoma and from 16 other spindle-cell tumors of the skin were stained for the presence of VIIIR-Ag, using an immunoperoxidase technique. Positive reactions were noted in all cases of Kaposi's sarcoma regardless of their histologic variations. Although the intensity of reaction was greater in cells that lined vascular channels and blood-filled clefts, many intertwining spindle cells contained VIIIR-Ag in their cytoplasm as well. Since VIIIR-Ag is elaborated only by endothelial cells and megakaryocytes, positive immunologic reaction for this factor in the cells of Kaposi's sarcoma strongly supports an endothelial derivation.

Antigens↗
PubMed1980

Prostatic origin of tumors. An immunohistochemical study.

An immunoperoxidase technic was used to localize prostatic acid phosphatase in a variety of primary and metastatic neoplasms. The aim was to explore the histogenesis of tumors affecting the prostate gland and to demonstrate the prostatic origin of metastases in various sites. A highly specific antiserum to prostatic acid phosphatase was raised in rabbits, and the peroxidase-antiperoxidase procedure was carried out on formalin-fixed paraffin-embedded routine pathology material. All specimens from the 37 cases of known primary and metastatic prostatic carcinomas stained positively for prostatic acid phosphatase, regardless of their histologic differentiation. None of the specimens from the 44 cases of proven nonprostatic primary and metastatic tumors stained positively for prostatic specific acid phosphatase. The data suggest that demonstration of prostatic acid phosphatase by the immunoperoxidase technic is a practical, sensitive, and specific test for the prostatic origin of an otherwise unclassifiable primary or metastatic neoplasm.

Acid Phosphatase↗
PubMed1980

Immunochemical demonstration of human chorionic gonadotrophin (hCG) in tissue of breast carcinoma.

Immunoreactive human chorionic gonadotrophin (hCG) was found in 9 of 65 surgically removed malignant breast tumours. The concentrations found ranged from 5 to greater than 500 mIU hCG/g tumour. Of the nine, seven extracts were assayed for total protein content; when expressed as mIU/mg extract protein, the hCG concentrations ranged from 0.28 - > 18.2 mIU/mg protein. Comparison of oestrogen receptor and hCG presence in the tumours showed no strong correlation. All tumours in which hCG was detected were shown to be of ductal origin. The hCG was measured by a beta-chain specific radioimmunoassay which measures only hCG even in the presence of high levels of other gonadotrophin hormones. In further study of these specimens an immunoperoxidase staining technique was used to stain for hCG in formalin-fixed sections of the same tumours assayed by RIA. This technique confirmed hCG in the positive specimens examined (tissue was available for 7 of the 8 positives) and no significant staining in any negatives. The hCG was shown to be localized within the cytoplasm and on the surface of the malignant cells but not in surrounding normal cells. This intracellular localization demonstrates that the malignant cells may produce hCG, rather than stimulating the pituitary to do so.

Breast Neoplasms↗
PubMed1980

Tissue immunoreactive prolactin hormone in breast cancer.

The prolactin contents of the cytosol extracts of 159 human mammary carcinomas was measured by specific radioimmunoassay. Of these extracts 157 contained measurable prolactin (detection limit, 7.8 ng/g tumor). The levels measured were in most cases greater than would be expected to be due to only serum contamination. The levels of prolactin did not correlate with either estrogen or progesterone receptor content of the tumors, or menopausal status of the patient.

Breast Neoplasms↗
PubMed1980

Immunochemical demonstration of immunoreactive insulin in human breast cancer.

The immunoreactive insulin contents of the cytosol extracts of 57 human mammary carcinomas were measured by specific radioimmunoassay. All of these extracts contained measurable insulin (detection limit 1.3 ng/g tumor). In some cases (23) the levels were quite greater than the normal human serum levels. Immunoperoxidase technique revealed the presence of immunoreactive insulin within the cytoplasm of tumor cells in 6 cases.

Adult↗
PubMed1979

Ectopic human chorionic gonadotropin in breast carcinoma.

Immunoreactive human chorionic gonadotropin (hCG) was found in 9 of 65 surgically removed malignant breast tumors. Concentrations ranged from 5 to greater than 500 mIU hCG/g tumor. hCG was measured by a beta-chain specific readiommunoassay. In further study of these specimens, an immunoperoxidase staining technique was used to stain for hCG in formalin-fixed sections. The hCG was shown to be localized within the cytoplasm and on the surface of the malignant cells.

Breast Neoplasms↗
PubMed1979

Fine-needle aspiration cytology in gynecologic oncology. I. Diagnostic accuracy.

A prospective study was undertaken to determine the diagnostic accuracy of fine-needle aspiration (FNA) cytology in gynecology. A total of 77 aspirations were performed on 74 patients. In 34 instances the purpose was to rule out or confirm a diagnosis of primary disease, and in 43 cases the procedure was used for suspected metastatic disease or disease recurrent after surgery, radiation therapy, and/or chemotherapy. Excellent correlation was noted between the cytologic and subsequent histopathologic diagnoses of 58 aspirations from patients who also underwent surgical biopsy. Two specimens were false negatives as the result of sampling errors. The applicability of FNA cytology in the field of gynecolotic oncology is discussed.

Adult↗
PubMedDate not supplied

Laparoscopically directed fine needle aspiration for the diagnosis of hepatocellular carcinoma: a safe and accurate technique.

Although ultrasonography and computerized tomography have been useful in the diagnosis of hepatocellular carcinoma, the extent of metastases and the presence of cirrhosis are not identified by these techniques before surgery. We studied 27 cases of presumptive hepatocellular carcinoma by means of laparoscopy and fine needle aspiration. Fine needle aspirates provided positive diagnoses of hepatocellular carcinoma in all patients. Laparoscopy complements imaging studies and allows selection of patients amenable to hepatic resection.

Biopsy, Needle↗
PubMedDate not supplied

Mapping of genetic deletions on the long arm of chromosome 22 in human pancreatic adenocarcinomas.

The molecular mechanisms of carcinogenesis in pancreatic cancer are still poorly understood, although the inactivation of tumor suppressor genes at multiple loci is suspected. We investigated the loss of heterozygosity (LOH) on chromosome 22 in pancreatic cancer by means of a PCR-based microsatellite analysis of archival paraffin-embedded histological sections in order to better define deleted region(s) and to test whether the NF-2 gene is involved. Using a panel of thirteen markers that spanned the long arm of chromosome 22, loss of heterozygosity was identified for at least one locus in 37% of investigated pancreatic adenocarcinomas. These deletions are clustered into two separate areas of the chromosome 22--one proximal to the NF-2 gene and one distal. The NF-2 gene itself is not involved. These regions are likely locations of tumor suppressor genes that may contribute to the development of pancreatic cancer.

Adenocarcinoma↗