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Biomedical subjects

M Muller

Publications and source records attributed to M Muller.

At least 217 records · Page 12Linked to original sources

The relationship between the rotation possibilities between femur and tibia and the lengths of the cruciate ligaments.

The system of the anterior (a) and posterior (p) cruciate ligaments and their distances between attachments to the femur (f) and tibia (t), respectively, as found in the knee joint of tetrapods is considered as a crossed four-bar linkage. Starting from a flat or curved tibial articulating surface the shape of the femoral articulating surfaces in principle can be derived (Huson, 1974; Menschik, 1974). The point of intersection (S) of the cruciate ligaments is the instantaneous centre of rotation and describes a curve during knee angulation; the centrode. Two centrodes are distinguished: S1 with the tibia stationary and S2 with the femur stationary. During leg bending the centrodes roll over each other, without sliding. Four series of knee-joint simulations were made. In series A, the femoral bar (distance between cruciate ligaments) was varied, in series B, the anterior cruciate ligament (so the length of a ligament). In series C and D, combinations of bar lengths were varied. The maximum leg rotation range was calculated from the lengths of the bars of the cruciate ligament system for each of the series A ... D. Also the leg rotation range for which the knee joint is mechanically stabilized by the cruciate ligaments was calculated. The stabilization criterium chosen was that a cruciate ligament may not become perpendicular (> 78.5 degrees) to the articulating surfaces. It was found that the cruciate ligaments alone cannot stabilize the knee joint adequately over the whole required range of leg movement. External structures are required to obtain full stabilization. As the femoral and tibial articulating surfaces never lie at the centrodes, considerable sliding occurs between them. It is suggested that a uniformly distributed sliding is essential for lubrication and so for a proper knee-joint functioning.

Animals↗

The angles of femoral and tibial axes with respect to the cruciate ligament four-bar system in the knee joint.

The system of the anterior (a) and posterior (p) cruciate ligaments and their distances between points of attachment to the femur (f) and tibia (t), respectively, as found in the knee joint of tetrapods is considered as a crossed planar four-bar linkage. The maximum lower leg rotation range follows from the lengths of the bars of the cruciate ligament system. The extreme flexion and extension positions of the leg can be derived when this range is combined with the angles between the femoral and tibial long axes and the cruciate ligament system. The "retroposition" of the femoral and tibial articulating surfaces is determined by the dimensions of the cruciate ligament four-bar system and the bending range of the leg. The above theory is applied to data from the knee joints of ten species of mammals and a bird.

Animals↗

[Practice of delayed autologous transfusion in Saint-Louis (Senegal)].

In Saint-Louis' Regional Hospital, transfusional risks and blood shortage led us to introduce the delayed autologous transfusion despite a great number of difficulties. Our study aimed at finding the most adapted technique to the conditions in an African peripheral hospital, and lasted four months (from April to July 1992). In this paper, our first results are given that encourage us to continue in this way.

Adolescent↗

Imaging of the portal venous system in patients with cirrhosis: MR angiography vs duplex Doppler sonography.

OBJECTIVE: To determine the relative performances of MR angiography and duplex Doppler sonography in the evaluation of the portal venous system in patients with portal hypertension, we compared two-dimensional time-of-flight MR angiography and duplex Doppler sonography in 60 sequential patients with cirrhosis. All 60 subsequently underwent liver transplantation. SUBJECTS AND METHODS: MR imaging and duplex Doppler sonography were performed on the same day, and in all patients the imaging findings were validated at surgical exploration. Five transplantation candidates had surgical portosystemic shunts in situ at the time of imaging. MR images were acquired during breath-holding, and flow in the portal vein was evaluated with presaturation bolus-tracking MR imaging. Sonography included color Doppler and pulsed gated Doppler images of the portal vein. MR angiograms and sonograms were analyzed independently and prospectively for patency of the portal vein, patency of surgical shunts, and grade and distribution of portosystemic collateral vessels. Scores on both imaging techniques were compared by using regression analysis. RESULTS: MR angiograms showed all five cases of portal vein occlusion and all 55 cases of patent portal veins. Sonograms showed three of five cases of portal vein occlusion and 52 of 55 cases of patent portal veins. The techniques concurred in assessing flow direction in 54 (90%) of 60 patients, and both procedures enabled detection of occlusive portal vein thrombus in one patient. We found a significant correlation (p < .001) between the grades of gastric varices detected with MR angiography and surgery but no significant correlation between the grades seen on sonography and found at surgery. Shunt patency in four of five patients and shunt occlusion in one were correctly assessed on MR angiograms, whereas three of these shunts were not visible on sonograms. CONCLUSION: Our results show that MR angiography is more reliable than duplex Doppler sonography for evaluating the portal venous system in patients with portal hypertension caused by cirrhosis.

Esophageal and Gastric Varices↗

Herpes simplex virus type 1 polypeptide ICP4 bends DNA.

ICP4, the major regulatory polypeptide of herpes simplex virus type 1, is expressed at the earliest stages of virus infection and is required for the activation of transcription from the majority of viral promoters. It is a DNA binding protein which specifically recognises bipartite sites related to the sequence ATCGTnnnnnCGG. In this report we show that both partially purified ICP4, and its isolated DNA binding domain, bend DNA at occupied binding sites. The apparent angles of bend at two different binding sites were very similar and in both cases the centre of the bend was very close to the binding site sequence.

Binding Sites↗

The infant mortality rate at Cherbourg aboriginal community: an update.

The infant mortality rate (IMR) at Cherbourg Aboriginal Community in south-eastern Queensland remained high from 1906 to about 1955-60, but since then has dropped from over 200/1000 live births in 1956-60 to 16/1000 live births in 1986-90, compared with the 1987 rate for Queensland (9.2/1000) and Australia (8.6/1000). The rapid improvement in the IMR was associated with the installation of a piped and chlorinated water supply, sewerage and an intensive campaign to eradicate intestinal worms. There has also been a change in community attitudes towards routine health practices and it is likely that this has been a major factor in the changes.

Humans↗

Bacterial metabolism of human polymorphonuclear leukocyte-derived arachidonic acid.

Evidence for transcellular bacterial metabolism of phagocyte-derived arachidonic acid was sought by exposing human blood polymorphonuclear leukocytes, prelabelled with [3H]arachidonic acid, to opsonized, stationary-phase Pseudomonas aeruginosa (bacteria-to-phagocyte ratio of 50:1) for 90 min at 37 degrees C. Control leukocytes were stimulated with the calcium ionophore A23187 (5 microM) for 5 min. Radiochromatograms of arachidonic acid metabolites, extracted from A23187-stimulated cultures and then separated by reverse-phase high-performance liquid chromatography, revealed leukotriene B4, its omega-oxidation products, and 5-hydroxy-eicosatetraenoic acid. In contrast, two major metabolite peaks, distinct from known polymorphonuclear leukocyte arachidonic acid products by high-performance liquid chromatography or by thin-layer chromatography, were identified in cultures of P. aeruginosa with [3H]arachidonic acid-labelled polymorphonuclear leukocytes. Respective chromatographic characteristics of these novel products were identical to those of two major metabolite peaks produced by incubation of stationary-phase P. aeruginosa with [3H]arachidonic acid. Production of the metabolites was dependent upon pseudomonal viability. UV spectral data were consistent with a conjugated diene structure. Metabolism of arachidonic acid by P. aeruginosa was not influenced by the presence of catalase, superoxide dismutase, nordihydroguaiaretic acid, ethanol, dimethyl sulfoxide, or ferrous ions but was inhibited by carbon monoxide, ketoconazole, and 1,2-epoxy-3,3,3-trichloropropane. Our data suggest that pseudomonal metabolism of polymorphonuclear leukocyte-derived arachidonic acid occurs during phagocytosis, probably by enzymatic epoxidation and hydroxylation via an oxygenase. By this means, potential proinflammatory effects of arachidonic acid or its metabolites may be modulated by P. aeruginosa at sites of infection in vivo.

Arachidonic Acid↗

Leukotriene B4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by Pseudomonas aeruginosa.

Human polymorphonuclear leukocytes (PMNL) metabolize the potent chemotaxin leukotriene B4 (LTB4) by omega-oxidation to 20-hydroxyl-LTB4 and 20-carboxy-LTB4. The ability of unstimulated human PMNL to metabolize exogenous LTB4 was found to be inhibited by pyocyanin, a phenazine derivative produced by Pseudomonas aeruginosa, in a dose-dependent manner. 1-Hydroxyphenazine (1-OHP), a metabolite of pyocyanin, was not inhibitory under identical conditions. The initial enzymic step in the conversion of LTB4 is catalyzed by an NADPH-dependent cytochrome, P-450. Reduction of the phenazine derivatives by NADPH was measured spectrophotometrically. Pyocyanin was reduced by NADPH in vitro in a pH-dependent manner, while 1-OHP was poorly or negligibly reduced under similar conditions. Formation of NADP+ was 20.3 +/- 1.8 nmol min-1 for pyocyanin (10 microM) at pH 5.5, compared with 0.6 +/- 0.2 nmol min-1 for 1-OHP (10 microM), while at pH 7.5 a value of 2.2 +/- 1.3 nmol min-1 was obtained for pyocyanin, with no detectable activity for 1-OHP. This indicates that inhibition of LTB4 omega-hydroxylase activity by pyocyanin might be achieved by competition for NADPH. Incorporation of exogenous 5-hydroxyeicosatetraenoic acid by PMNL into lipid pools was not affected by either phenazine derivative. The ability of bacterial pyocyanin to limit the omega-oxidation of LTB4 may have important implications for PMNL LTB4 receptor status and chemotaxis in vivo.

Humans↗

[The contribution of unit managers in the education of student nurses].

The contribution of a group of unit managers towards student education, as well as the problems related thereto, has been investigated contextually by means of an explorative descriptive study, utilising structured interviews. The results indicate that, although unit managers have a positive attitude towards student education, they lack insight regarding the programme content, as well as their own informal educational function in the clinical situation. Lack of time and inadequate liaison between the college and unit managers seem to aggravate this problem. The recommendations include the design and implementation of inservice programmes for unit managers, improved liaison between educational and unit staff, as well as role clarification concerning their informal education responsibilities.

Curriculum↗

Plasma polymerization and grafting of ethylene oxide on polysiloxane surfaces.

Polysiloxane films were prepared by plasma polymerization and dip coating. Upon these films PEG was grafted by conventional methods, and ethylene oxide by plasma activation. Before and after grafting the films were analyzed by contact angle and ESCA measurements as well as by FTIR-ATR-spectroscopy and TOF-SIMS. Furthermore results of protein adsorption measurements are reported. The polysiloxane structures obtained by the plasma polymerization of HMDSO are rather similar to those ones yielded by dip coating. The two types of grafting applied on the polysiloxane substrates led to different structures of the grafted layers as was revealed by TOF-SIMS spectra.

Adsorption↗

Co-operative binding of the glucocorticoid receptor DNA binding domain is one of at least two mechanisms for synergism.

Steroid induction of responsive genes functions through the synergistic activity of steroid receptor binding sequences with adjacent binding sites either for other transcription factors or for further steroid receptors. Analysis of the human glucocorticoid receptor revealed that the DNA-binding domain of the receptor is sufficient to mediate co-operative binding to adjacent receptor binding sites. This is a novel feature of the domain in addition to its DNA-binding, trans-activating and trans-repressing properties. Chimaeric proteins containing the N- or C-terminal receptor halves fused to the GAL4 DNA-binding domain do not co-operate in DNA-binding, however they do functionally synergize. Thus, at least two mechanisms contribute to the synergism of the human glucocorticoid receptor bound to two adjacent receptor binding sites.

Base Sequence↗

Embryology of the hair follicle.

A study of hair follicle development has enabled us to differentiate four stages by relating them to corresponding gestation ages. Stage I of the hair bud is characterized by an epiblastic proliferation penetrating the subjacent mesenchyma at the extremity of which mesenchymal cells accumulate. This appears, at the level of the lower lip, before the 11th week of intrauterine life. Stage II of the hair bulb is reached on the 12th-13th week of intrauterine life, as soon as the distal extremity of the hair bud, which has extended, becomes depressed at the mesenchymal papilla level. Stage III is defined by the observation of hair cone and rough sebaceous glands on the 15th week of intrauterine life. Finally, stage IV shows a differentiated sebaceous gland with a hair which comes through the skin surface on the 18th week of intrauterine life.

Fetus↗