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Biomedical subjects

M Motomiya

Publications and source records attributed to M Motomiya.

At least 145 records · Page 8Linked to original sources
PubMed1977

Role of the posterior hypothalamus in the development of acute brain swelling.

Destructive stereotaxic lesions were made in the posterior hypothalamus, unilaterally or bilaterally, in 26 dogs. In 21 dogs the intracranial pressure (ICP) was maintained in normal range, and in five dogs the ICP was artificially elevated to 300 to 400 mm h2o, preceding the procedures to the hypothlamus. Arterial oxygen and carbon dioxide pressure ((PaO2 and PaCO2) were maintained in the normal range. Before and after each procedure, systemic arterial pressure (SAP) was elevated by intravenous injection of norepinephrine (5 X 10(-3) mg/kg) to determined whether the ICP increases coincidentally with elevation of the systemic arterial pressure. The intracranial pressure/mean arterial pressure ratio of elevation by injection of norepinephrine was not significant regardless of the level of the ICP, or of uni-or bilateral lesions of the hypothalamus. The authors conclude that dysfunction of posterior hypothalamus does not play a specific role in the development of vasomotor paralysis leading to acute brain swelling, under conditons of normal or moderately raised ICP with normal PaCO2 and Pato2 levels.

Animals↗
PubMed1976

Experimental pulmonary emphysema in Syrian hamsters.

Syrian hamsters were exposed to the aerosol of a 5% solution of papain in the presence of cysteine and ethylenediamine-tetraacetic acid. Pulmonary emphysema in hamsters was observed after exposure to papain for two to three hours. There was no change with time in the severity of emphysema from the second through the fourth weeks after exposure to papain. Pathogenesis of pulmonary emphysema was discussed in relation to proteolysis of glycosaminoglycan-protein complex.

Animals↗
PubMed1975

On acid glycosaminoglycans (mucopolysaccharides) in pleural effusion.

The concentration of acid glycosaminoglycans (mucopolysaccharides) in pleural fluid was found to be in the range of 4.0 to 60.3 mug per ml. By chromatographic analysis and by electrophoresis of the eluates, in combination with enzymatic digestion, it was found that pleural fluid contained protein-free hyaluronic acid, protein-bound hyaluronic acid, undersulfated chondroitin sulfate A(C), chondroitin sulfate A(C), protein-bound chondroitin sulfate A(C), and dermatan sulfate. The composition of acid glycosaminoglycans in pleural fluid seems to reflect the metabolism of the pleural cavity.

Adult↗
PubMed1975

Biochemical characterization of hyaluronic acid from a case of benign, localized, pleural mesothelioma.

The tissue of a benign, localized, pleural mesothelioma was digested with protease, and the crude polysaccharide was fractionated by a column of Dowex-1 (Cl-form). The eluate from the column was electrophoresed and incubated with various mucopolysaccharide lysases. Based on the results of column chromatography, electrophoresis, and enzymatic digestion, it was found that hyaluronic acid was the major constituent of the glycosaminoglycans in pleural mesothelioma. The hyaluronic acid from pleural mesothelioma seemed to be identical in structure with that from human umbilical cord; however, the hyaluronic acid from mesothelioma was eluted before that from human umbilical cord when fractionated by the column of Sepharose 4B, suggesting a differnence in molecular size between the two. Also, evidence was obtained for the presence of dermatan sulfate and heparan sulfate.

Chromatography, Ion Exchange↗
PubMed1975

Increase of dermatan sulfate in a case of pulmonary fibrosis.

The quantity and composition of acid glycosaminoglycans (mucopolysaccharides) were compared between a fibrotic lung and normal lungs. The acid glycosaminoglycans were isolated by proteolysis, fractionation with ethanol and precipitation with cetylpyridinium chloride, and were identified by chromatography, electrophoresis and incubation with mucopolysaccharide-lyases. Evidence was obtained for the presence of hyaluronic acid, chondroitin sulfate A(C), dermatan sulfate and heparan sulfate. Quantitation of individual glycosaminoglycans by using specific mucopolysaccharide-lyases revealed that the quantity of dermatan sulfate in the fibrotic lung exceeded that in the normal lungs.

Chondroitin↗
PubMed1975

[Transoral approach for the treatment of atlanto-axial dislocation with the Os odontoideum and obstruction of the bilateral vertebral arteries (author's transl)].

The two approaches are well-known in the surgical treatment of atlanto-axial dislocation, the one is the anterior and the other is the posterior approach. The indication of these methods is still controversial. This report concerns with one case of atlanto-axial dislocation with the separate odontoid and bilateral obstruction of both vertebral arteries, which was successfully treated by transoral decompression and fusion between the bodies of the atlas and axis. The reasons why we chose this method were: (1) the spinal cord decompression was necessary because of the presence of compression of the cord by the separate odontoid process, (2) the spinal fusion had to be performed to stabilize the atlanto-axial articulation, (3) it was necessary to avoid the damage of collateral circulations to the brainstem through the deep cervical arteries demonstrated by bilat vertebral angiography. The posterior approach is highly susceptible to injure these collateral circulations.

Adolescent↗
PubMed1975

[On glycosaminoglycans (mucopolysaccharide) in a case of pulmonary fibrosis (author's transl)].

Glycosaminoglycans from a case of pulmonary fibrosis (desquamative interstitial pneumonia of usual type) was analyzed by means of proteolytic digestion, solvent fractionation, column chromatography on anion exchanger, electrophoresis and enzymatic digestion. The result suggested an increase in dermatan sulfate and possibly of heparan sulfate in the fibrotic lung as compared with the normal. It is preferable to take the samples for analysis from a freshly excised specimen of the lung, because there was a sign of degradation of glycosaminoglycan due to storage of the specimen in a solution of formalin.

Culture Techniques↗