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Biomedical subjects

M Motomiya

Publications and source records attributed to M Motomiya.

At least 127 records · Page 7Linked to original sources
PubMed1982

The stress-strain relationship of the diastolic cardiac muscle and left ventricular compliance in the pressure-overload canine heart.

The relation between left ventricular (LV) compliance, chamber geometry and LV muscle compliance in pressure-overload hearts was studied. The diastolic pressure (P)-volume (V) relationship was evaluated at different ventricular volumes of isovolumic beat in 4 pressure-overload hypertrophied (H) and 5 normal (N) hearts. In H, the ratio of the LV volume at the EDP of 15 mmHg to LV weight (LVW) was 0.34 +/- 0.08 (SD) ml/g (N: 0.63 +/- 0.07, p less than 0.01). Thus the occurrence of concentric hypertrophy due to pressure-over-load was confirmed. The P-V relation was expressed as, delta V = a - b.exp (-cP), where a, b and c were constants, delta V = V - V0, and V0 was the initial volume at EDP = 0 cmH2O. Since this equation leads to the relation: d delta V/dP = c (a - delta V), c may be regarded as a parameter which represents the LV chamber compliance. In H, c was 0.074 +/- 0.024 (SD) mmHg-1 (N: 0.109 +/- 0.008 mmHg-1, p less than 0.01). Therefore, H was found to show decrease of LV compliance. The biaxial stress (sigma)-strain (epsilon) relation was derived from the P-V relation based on large deformation theory. The sigma-epsilon relationships of myocardium in H were not apparently different from that in N. However, there was a close correlation between c and LV volume (at EDP = 15 mmHg)/LVW (r = 0.83). Thus it was found that the change in LV compliance in H resulted from the change in LV chamber geometry.

Animals↗
PubMed1982

Evidence for host-mediated antitumor activity in the treatment of experimental tumor with a hot water extract from BCG.

Studies were undertaken for the evaluation of the mechanism responsible for antitumor effects of hot water extract (HWE) of BCG on murine ascites tumor cells. a) The viability of tumor cells has remained unchanged after incubation of tumor cells in the medium containing HWE. b) There was no difference in survival time between mice which had received tumor cells treated as in (a) and the control mice. c) Silica, carragheenan and cortisone acetate reduced the antitumor effect of HWE in ddY mice and C3H/He mice which had been inoculated with Sarcoma 180 cells or MH134 cells. d) The growth of tumor cells was retarded when they were inoculated subcutaneously together with peritoneal cells from HWE-injected mice. These results indicate the participation of host-mediated antitumor activity when neoplasms were treated with HWE.

Animals↗
PubMed1982

[A case of generalized tuberculosis].

A 52 years old female with suspected malignancy was hospitalized because of fever and lymph node swelling. In spite of lymph node biopsy, bone marrow aspiration, blood culture and other diagnostic procedures, no clue to definite diagnosis was obtained. During the course of hospitalization, abscesses with fistulae appeared and tubercle bacilli were demonstrated in the pus from a subcutaneous abscess. Thus, the diagnosis of tuberculosis was established. Finally the patient died of massive bleeding as a result of perforation into thoracic aorta of the zone of necrosis of esophageal wall. It is to be emphasized that tuberculosis should be kept in the list of differential diagnosis of continuous fever.

Antitubercular Agents↗
PubMed1981

Chemical and biological properties of subfractions of hot-water extract from delipidated BCG.

a hog-water extract from delipidated Mycobacterium bovis strains BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfractions thus obtained were investigated. A subfraction precipitated at 20% ethanol contained less neutral sugar and more ninhydrin-positive substance than other subfractions. Only the guinea pigs that were treated with 20% ethanol-precipitate showed no humoral antibody response to egg white albumin, despite positive skin reaction. The subfractions precipitated at low concentrations of ethanol exhibited a significant antitumor activity in ddY and C3H He mice inoculated intraperitoneally with tumor cells. These results suggest that the hot-water extract from delipidated BCG consists of subfractions with different chemical and biological properties.

Adjuvants, Immunologic↗
PubMed1981

Lipid composition of adipose tissue from "membranous lipodystrophy".

The lipid composition of the adipose tissue from the bone marrow and that of the subcutaneous depot fat of four patients of membranous lipodystrophy were investigated. It was found that the lipid composition of the samples from the cases of membranous lipodystrophy was almost similar to that in controls. Also discussions were made on mechanism related to the accumulation of abnormal depot fat.

Adipose Tissue↗
PubMed1980

A biochemical study on bronchopulmonary lavage fluid from a case of pulmonary alveolar proteinosis.

A 44-year-old male was hospitalized, because of the presence of abnormal shadows on chest x-ray film. Diagnosis of pulmonary alveolar proteinosis was established by examination of a specimen obtained by transbronchial biopsy. Therapeutic bronchopulmonary lavage was performed twice. As a result, there was an improvement of radiological findings on chest x-ray film. Then a biochemical study was carried out with pooled lavage fluids. Cellular debris were removed by centrifugation. Lipids were extracted with a 2:1 mixture of chloroform and methanol. Individual phospholipids were identified by column chromatography and thin layer chromatography. Fatty acids were identified by gas chromatography. It was found that phosphatidyl choline (lecithine) was the major component of phospholipids. On the other hand, myristic acid of the molecule of phosphatidyl choline was found to constitute 19.6% of the total fatty acids. This patient lives a normal life as of Dec. 1980.

Adult↗
PubMed1979

Significance of the quantification and demonstration of hyaluronic acid in tissue specimens for the diagnosis of pleural mesothelioma.

Hyaluronic acid in pleural tissues from patients with mesothelioma, carcinoma, and asbestosis of the lung was quantified by using specific glycosaminoglycan-degrading enzymes. In all cases of pleural mesothelioma, the quantity of hyaluronic acid in mg/g of dry tissue was at least 0.10 mg, whereas carcinomatous pleural tissue and pleura in asbestosis contained 0.02 to 0.03 mg/g of dry tissue.

Asbestosis↗
PubMed1979

A biological study on hot-water extract from delipidated Mycobacterium bovis strain BCG.

The biological activity of a hot-water extract from delipidated BCG, designated as HSA (Hot-water Soluble Adjuvant), was investigated. The HSA did not induce hyperreactivity to bacterial endotoxin. The hot-water extract from which nucleic acids had been removed by streptomycin (SM-HSA) was found to enhance the delayed-type hypersensitivity as evidenced by the results of footpad reaction of mice. The HSA and SM-HSA could be injected to mice by an intraperitoneal route for 20 consecutive days without undesirable side effects. A comparative study was made on the effects of HSA in relation to the duration and doses of treatment with HSA using the ddI mice inoculated with Sarcoma-180. The most remarkable effect was observed when 0.25 mg of HSA had been injected for 20 consecutive days. Also SM-HSA was found to exert antitumor activity when applied in the same manner as above. These results suggest that the presence of nucleic acids is not related to the biological and antitumor activities of the hot-water extract.

Adjuvants, Immunologic↗
PubMed1979

[Biosynthesis of hyaluronic acid in parietal pleura of the rabbit (author's transl)].

The parietal pleura of rabbits was incubated with 14C-glucosamine. It was found that 14C-glucosamine was incorporated into the fraction of crude glycosaminoglycans. Then the crude glycosaminoglycans were fractionated by using specific mucopolysaccharide-lyases (hyaluronidase from streptomyces hyalurolytics, chondroitinase AC and chondroitinase ABC). As a result, evidence was obtained that hyaluronic acid was synthesized in parietal pleura and was released into the surroundings.

Animals↗
PubMed1978

Antitumor activity of hot-water extract from delipidated BCG.

The antitumor activity of hot-water extract of delipidated BCG was investigated in mice inoculated with Sarcoma-180 cells and Ehrlich carcinoma cells, respectively. The hot-water extract was found to be effective when administered after and ineffective when administered before the inoculation of tumor cells. When this extract was given with anticancer drugs, such as Mitomycin C and cyclophosphamide, a combined effect was obtained in the treatment of Sarcoma-180 and of Ehrlich carcinoma.

Adjuvants, Immunologic↗