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Biomedical subjects

M Morimatsu

Publications and source records attributed to M Morimatsu.

At least 271 records · Page 15Linked to original sources
PubMed1985

Adult rat heart cell culture. The morphological study of tissue fragments on various environments in culture.

This paper reports a modification of Jacobson's method for culturing adult rat heart cells after mechanical and enzyme treatment. For heart cell dissociation, it is useful to wash tissue fragments 3 times with saline A solution after enzyme treatment, and it is important to change the medium immediately after culturing heart cells in order to prevent myocardial cells from degeneration caused by the enzyme solution and toxins in the cell debris.

Animals↗
PubMed1985

[Ultrasonographic study of the gastric wall].

Ultrasonographically, the normal gastric wall is delineated in 5 layers, 2 hypoechoic layers and 3 hyperechoic layers. To elucidate the sources of the reflected ultrasonic picture above, we infused India ink into the ultrasonographic wall of the resected human stomach. After the ultrasonographic examination, each stomach was examined histologically, and findings were compared with those of ultrasonography. The results were as follows: Ultrasonographically, an irregular hypoechoic nodule was seen in the 3rd layer, i.e., the middle hyperechoic layer, in cases in which India ink was seen in the submucosal layer histologically. Namely, the ultrasonographic 3rd layer was considered to be consistent with the submucosal layer.

Humans↗
PubMed1985

Difference in the inhibitory effect of linoleic acid hydroperoxide on prostacyclin biosynthesis between cultured endothelial cells from human umbilical cord vein and cultured smooth muscle cells from rabbit aorta.

The biosynthesis of prostacyclin in cultured endothelial cells from human umbilical cord vein was inhibited by linoleic acid hydroperoxide at a concentration of 0.1 nmol/ml (in terms of malondialdehyde), while that in cultured smooth muscle cells from rabbit aorta was inhibited only at a higher concentration of the hydroperoxide (5.0 nmol/ml).

Animals↗
PubMed1985

Difference in susceptibility to injury by linoleic acid hydroperoxide between endothelial and smooth muscle cells of arteries.

Electron microscopic examination of the effect of linoleic acid hydroperoxide on cultured smooth muscle cells from human umbilical artery revealed that incubation of the cells with 5.0 nmol/ml (in terms of malondialdehyde) of the hydroperoxide for 3 h caused a decrease in the electron density of the mitochondrial matrix and that dilatation of the rough-surfaced endoplasmic reticulum occurred when the cells were incubated with 10 nmol/ml of the hydroperoxide. These concentrations are higher by one order of magnitude than those required for the same effects on cultured endothelial cells from human umbilical vein. A similar difference in susceptibility to injury by linoleic acid hydroperoxide was found between cultured endothelial and smooth muscle cells from fetal calf aorta. The effects of the hydroperoxide on the respiration of these cells paralleled the observed morphological changes.

Animals↗
PubMed1984

Tubular adenoma with squamous metaplasia of the sigmoid colon: a case report.

A case of tubular adenoma with squamous metaplasia of the sigmoid colon in a 44-year-old female is reported. The tumor was a pedunculated polyp with a size of 1.2 X 1.7 X 1.7 cm. Histologically, the tumor showed tubular adenoma with focal squamous metaplasia. Borderline atypia was noted in a part of adenoma without squamous metaplasia and squamous component. It is supposed that squamous epithelial polyp, squamous cell carcinoma, adenosquamous carcinoma, and mucoepidermoid carcinoma may be derived from colonic adenoma with squamous metaplasia. Only six cases of the adenoma with squamous metaplasia of the large intestine have been reported previously in the literature.

Adenoma↗
PubMed1984

Leiomyosarcoma of the breast--a case report and an electron microscopic study.

We treated a fifty six-year-old woman with leiomyosarcoma of the breast. Light microscopy showed typical findings of leiomyosarcoma and electron microscopy confirmed the smooth muscle origin of the tumor. The patient is well without evidence of metastases or local recurrence of the tumor fifty-five months after radical mastectomy.

Basement Membrane↗
PubMed1984

Variant endothelial cells from human carotid artery in culture.

Endothelial cells were cultured from the carotid artery with thickened intima comprised of two to five layers of smooth muscle cells, isolated from a 19-yr-old female, who died from an accident. The cells were grown and subcultured in Medium 199 supplemented with 20% heat inactivated fetal bovine serum. The cells are still viable at present, the 22nd passage. The cultured cells were found to have the following characteristics: existence of Factor VIII-related surface antigen and prostacyclin synthesis slightly less than that for typical endothelial cells. The most outstanding feature was the formation by an individual cell of a single ring, and a composite ring formed by two to five cells. Neither the synthesis of an angiotensin converting enzyme nor that of a Weibel-Palade body could be detected by electron microscopy. The cultured cells possessed only a few characteristics specific for typical endothelial cells and were designated as variant endothelial cells.

Adult↗
PubMed1984

Anaplastic giant cell tumor with mucinous cystadenocarcinoma of the ovary. Origin of the giant cells.

The present case is that of a 27 years old woman who had received right oophorectomy for ovarian cancer nine years previously. The histological diagnosis at that time was mucinous cystadenocarcinoma with anaplastic areas. The patient expired ten years after oophorectomy due to widespread metastases of the tumor. Review of the histological slides revealed ovarian giant cell tumor which was considered to be of epithelial origin differing from those appearing in giant cell tumor of bone.

Adult↗
PubMed1984

An ultrastructural study of extraskeletal Ewing's sarcoma.

A case of extraskeletal Ewing's sarcoma was studied ultrastructurally, cytologically, and cytochemically. This tumor was, as in that of osseous origin, composed of densely packed highly undifferentiated blastemic cells and so-called "darker cells." The principal tumor cells were undifferentiated cells, which were characterized by the scant cytoplasm containing a variable amount of glycogen granules, large round or oval nuclei with dispersed chromatin, and rudimentary cytoplasmic junctional complexes. Some of the darker cells seemed to show somewhat histiocytic differentiation from principal blastemic cells, but the others were degenerating. Cytochemically, no tumor cells revealed the presence of peroxidase, lysozymes, and Factor VIII-related antigen. It is likely that both Ewing's sarcomas of osseous and extraosseous origin are defined as undifferentiated sarcoma merely characterized by the presence of glycogen.

Adolescent↗
PubMed1984

Pigmented storiform neurofibroma.

A case of pigmented storiform neurofibroma is presented. The lesion was present in the right loin of a 35-year-old Japanese woman. Grossly, a round, slightly elevated, approximately 1.5 cm in diameter tumor showed a red-purplish coloration of the central part of the overlying skin without ulceration. The characteristic storiform pattern and scattered melanin-containing cells were the relevant features for histological diagnosis. To our knowledge, our case is the second reported case in Japan. The histogenesis and its relationship with tumors of neural crest origin is briefly discussed.

Adult↗