Search PubMed⌕ Search

Biomedical subjects

M Matsui

Publications and source records attributed to M Matsui.

At least 559 records · Page 31Linked to original sources

Interaction of chromatin components with nuclear 5.0 S RNA fraction that stimulates RNA synthesis.

Chick liver nuclear 5.0 S RNA, which stimulates RNA synthesis on chromatin, binds preferentially to the deoxyribonucleoprotein in homologous chromatin. The proteins found in the isolated deoxyribonucleoprotein-5.0 S RNA complex are total amount of both H4 and H3 histone, about 20% of nonhistone protein and about 50% of both H2a and H2b histone found in the original chromatin. Cesium chloride equilibrium centrifugation of the deoxyribonucleoprotein-5.0 S RNA complex after fixation with formaldehyde shows that the 5.0 S RNA is bound to certain proteins (acid-soluble and -insoluble) in the complex. The stimulation of RNA synthesis by the nuclear RNA fraction is due to the conversion of inaccessible region of DNA to RNA polymerase into an accessible one and presumably not due to an increase in the number of binding sites for RNA polymerase in the chromatin. The release of non-histone protein from chromatin following the addition of the nuclear RNA fraction is also briefly discussed.

Adenosine Triphosphate↗

Gas chromatographic determination of methylguanidine, guanidine and agmatine as their hexafluoroacetyletonates.

Seven derivatives of each of methylguanidine, guanidine and agmatine have been prepared, and the specificity and volatility of their gas chromatographic detection have been studied. The hexafluoroacetylacetonates have been found to be the most specific for the three guanidines, and are highly sensitive to alkali flame ionization and electron-capture detections. These derivatives are also fairly resistant to hydrolysis occurring in the derivatization process.

Agmatine↗

Mode of increase in cell wall polysaccharides in synchronously growing Sachharomyces cerevisiae.

The mode of increase in cell wall polysaccharides of yeast (glucan and mannan) during cell cycle was analyzed using cell wall samples obtained from a synchronous culture. The increase in mannan and total glucan proceeded almost linearly throughout the cell cycle except for a short period of their leveling off at the time of cell division. However, the constituents of glucan behaved characteristically: Alkalisoluble glucan and insoluble glucan increased mainly in the former and the later half of the cell cycle, respectively.

Cell Cycle↗

Radical scavenging reactions of alpha-tocopherol. II. The reaction with some alkyl radicals.

alpha-Tocopherol was reacted with some alkyl radicals (ethyl, n-propyl, iso-propyl, n-butyl, and sec-butyl radical) to study its radical scavenging reactivity. The two types of products (alkyl ethers of alpha-tocopherol and cyclohexadienones) were obtained on treatment of each radial. These structures were determined by the spectral analysis. It was observed that alpha-tocopherol is very sensitive to the alkyl racidals and that the yields of the cyclohexadienones are decreased and that of the alkyl ethers are not much varied with an increase of carbon numbers of the alkyl radicals.

Chemical Phenomena↗