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Biomedical subjects

M Matsui

Publications and source records attributed to M Matsui.

At least 433 records · Page 24Linked to original sources
PubMed1989

[Classification of diabetic retinopathy and application standard of photocoagulation].

It has been confirmed in some prefectures in Japan that severe diabetic retinopathy became the leading cause of blindness. The authors described classification of diabetic retinopathy and discussed that in order to prevent blindness due to diabetic retinopathy, it was absolutely necessary to place fundus examination in the management system of diabetes and also to include photocoagulation therapy in the management system of diabetic retinopathy. Application of fundus examination in the management system of diabetes was described correspondingly in cases with or without retinopathy and in cases of each type of diabetic retinopathy. Indication of photocoagulation for diabetic retinopathy was discussed, according to types of the retinopathy, such as maculopathy, preproliferative retinopathy or early stage of proliferative retinopathy. Standard technique of photocoagulation in each type of the retinopathy was discussed. Particularly it was stressed that the panretinal photocoagulation should not be performed in a single session, but should be performed separately in four or five sessions at one week interval.

Diabetic Retinopathy↗
PubMed1989

[Mutagenicity of steviol: an analytical approach using the Southern blotting system].

Steviol is the aglycone of stevioside, which is a non-caloric sugar substitute commonly used in Japan. Our previous studies and Pezzuto et al. have demonstrated that steviol is mutagenic after metabolic activation in the forward mutation assay using Salmonella typhimurium TM677 (TM677), whereas it is non-mutagenic in the reverse mutation assay (Ames test) using S. typhimurium TA 100, TA98, TA102 and TA97. There is the possibility, therefore, that activated steviol selectively induces a deletion or insertion of more than one base pair which cannot be detected by strains commonly used in the Ames test. In this study, we confirmed first that the 8-azaguanine (8-AG) resistance of the TM677 mutants appears to reside in the chromosomal gpt gene, since it can be complemented by the gpt gene of E. coli (Ecogpt). The chromosomal DNA of TM677 and TM677 mutants were digested by several restriction enzymes: BamHI, Sau3AI, AluI, TaqI, HaeIII, HpaII and RsaI, and analyzed by the Southern blot hybridization technique with a probe to the gpt gene DNA of E. coli. No significant differences in DNA fragment length, however, were formed between the wild type and spontaneous or steviol-induced mutants.

Azaguanine↗
PubMed1989

[Treatment of chlamydial urethritis--studies on clinical effects of ofloxacin].

Clinical effects of ofloxacin (OFLX) in the treatment of the patients with chlamydial urethritis was studied. OFLX was administered at a dose of 200 mg, three times daily (600 mg) for 14 days. In all of the 26 patients with chlamydial urethritis, C. trachomatis was eliminated in 7 to 14 days after the start of administration. The subjective clinical symptoms such as pain on urination and abnormal urethral feeling was disappeared in all cases within 7 days after the administration. The objective clinical symptoms, urethral discharge, polymorphonuclear leukocyte (PMNL) in urethral discharge and PMNL in first urine were improved in 94, 68, 91% respectively on 14 days after the administration. Overall clinical efficacy rate of OFLX on 7 days, 14 days and 21 days after the administration in this study was 63, 82, 91% respectively. Whereas subjective symptoms of side effects were noted in 2 patients (7.6%), any additional medical care was needed in none of them. Because of the marked improvement of clinical symptoms and the safety administration, OFLX could be the first regimen to be chosen for the treatment of the patients with chlamydial urethritis.

Adult↗
PubMed1989

[Effects of intravitreal steroid injection on rabbit eye].

In a previous study by the authors, it was determined that irrigation solutions containing an anti-prostaglandin agent had the capability of preventing the occurrence of blood-ocular barrier disruptions, retinal edema and post-operative inflammation following vitrectomy. However, not only prostaglandins but also leukotrienes, noted for their migratory effect on leukocytes, have cell migratory effect on the retinal pigment epithelial cells. Therefore, irrigation solutions containing an anti-prostaglandin agent and a steroid are expected to have the capability of preventing the occurrence of proliferative vitreoretinopathy and post-operative inflammation after vitrectomy. In this experiment, two kinds of steroids were injected into rabbit vitreous cavities in order to evaluate toxicity and to establish the toxic intravitreal dose of steroids on intraocular tissue. After paracentesis, 36 eyes of 18 rabbits were injected with dexamethasone sodium phosphate or betamethasone sodium phosphate, suspended in 0.3 ml of distilled water. Each steroid has the same molecular weight and almost the same clinical effects. The four eyes of two rabbits received 0.3 ml of physiologic saline as a control. All eyes were observed histologically seven days after the injection. It was proved that each steroid had almost the same toxicity for the same concentration and all steroids at dose less than five mg did not show toxicity on intraocular tissue. With all steroids, 10mg produced localized retinal degeneration at the inferior region of the posterior fundus. Twenty mg caused more extensive retinal degeneration. At a dose of 80 mg each steroid caused corneal edema, degeneration of ciliary bodies, fibrin formation in the vitreous body and degeneration of retinal cells throughout the retina. Considering the osmolarity and pH of each steroid solution, it was concluded that less than five mg of each of the steroids did not cause retinotoxicity, whereas more than 10 mg of any steroid did cause retinotoxicity.

Animals↗
PubMed1988

Isolation of human cDNA clones of myb-related genes, A-myb and B-myb.

cDNA clones of the myb-related genes A-myb and B-myb were obtained by screening human cDNA libraries. The predicted open reading frame of B-myb could encode a protein of 700 amino acid residues. Although the C-terminal end has not been cloned yet, an almost entire coding region of A-myb, which is 745 amino acid long, was determined. The A-myb and B-myb proteins are highly homologous with the myb protein in three regions. Domain I, which is 161 amino acid long, is well conserved in the myb gene family. The homology between human-myb and A-myb in domain I is 90% at the amino acid level. Domain II, which is about 85 amino acid long, is less well conserved. Although it is a short stretch, domain III is found in the C-terminal region. The mRNAs of A-myb and B-myb were 5.0 and 2.6 kb, respectively. The mRNA expression pattern of the myb gene family in various tumors is presented.

Amino Acid Sequence↗
PubMed1988

Synergistic in vitro and in vivo anti-tumor effect of daunomycin-anti-96-kDa melanoma-associated antigen monoclonal antibody CL 207 conjugate and recombinant IFN-gamma.

The mAb CL 207 recognizes a 96-kDa melanoma-associated Ag. The latter is not modulated by antibody but is highly susceptible to induction by immune IFN-gamma on human carcinoma and melanoma cells. The mAb CL 207 does not mediate C- and cell-dependent lysis of human carcinoma and melanoma cells. Conjugation of daunomycin with mAb CL 207 causes a slight reduction of its immunoreactivity and affinity but does not affect its serologic specificity or the toxicity of daunomycin. In combination with IFN-gamma, the daunomycin-mAb CL 207 conjugate displays a selective in vitro and in vivo toxic effect on tumor cells that express the 96-kDa MAA. The synergistic effect of IFN-gamma and daunomycin-mAb CL 207 conjugate is specific. The in vivo toxicity is influenced by the interval between injection of human tumor cells into nude mice and that of IFN-gamma and daunomycin-mAb CL 207 conjugate. The present results suggest that the 96-kDa melanoma-associated Ag may be a useful model to investigate the combined use of IFN-gamma and mAb for the selective destruction of tumor cells.

Animals↗
PubMed1988

Studies on the genetic linkage of bilirubin and androsterone UDP-glucuronyltransferases by cross-breeding of two mutant rat strains.

Gunn rats, which have defects in bilirubin and 4-nitrophenol UDP-glucuronyltransferases (GT), were crossed with LA Wistar rats with a defect in androsterone GT. The F1 hybrids showed normal GT activities towards androsterone, bilirubin and 4-nitrophenol, demonstrating that Gunn and LA ('low activity') Wistar rats inherit a homozygous dominant trait for androsterone GT and bilirubin GT respectively. The F2 progeny showed four different combinations of bilirubin and androsterone GT activities: defects in both GT activities, a single defect in bilirubin GT activity, a single defect in androsterone GT activity and two normal GT activities. They were segregated in the approximate ratio of 1:3:3:9, which is compatible with Mendel's Principle of Independent Assortment. These results provide evidence that androsterone GT and bilirubin GT are located on different chromosomes. In the F2 generation, defective bilirubin and 4-nitrophenol GT activities were not segregated, indicating that these two mutant genes are closely linked on the same chromosome.

Androsterone↗
PubMed1988

A monoclonal antibody to the carbohydrate chain on human hepatocellular carcinoma-associated antigen which suppressed tumor growth in nude mice.

There have been few reports stating that monoclonal antibody alone inhibits human solid tumor growth in vivo. The present study demonstrated that monoclonal antibody S1 (IgG2a), which recognized the antigenic determinant of the carbohydrate moiety, showed antibody-dependent cell (or macrophage)-mediated cytotoxicity (ADCC or ADMC) in conjunction with murine splenocytes of both BALB/c and athymic mice. In vivo experiments demonstrated that the antibody S1 clearly prolonged the survival of athymic mice which had been inoculated with a human liver carcinoma cell line. In addition, the antibody S1 significantly suppressed the human hepatoma line transplanted s.c. into nude mice. 125I-Labeled monoclonal antibody S1 revealed that the antibody accumulated significantly in the tumor mass. Many mononuclear cells were observed surrounding tumor cells when the antibody was given. This model system might be useful for analyzing the ADCC (or ADMC) mechanism in vivo.

Animals↗
PubMed1988

Effects of administration of N-nitrosodialkylamines and N-nitrodiethylamine on hepatic UDP-glucuronosyltransferase activity in Wistar rats.

N-Nitrosodiethylamine (NEN) and N-nitrodiethylamine (NEA) are carcinogens and in vitro activators of hepatic UDP-glucuronosyltransferase (GT) toward 2-aminophenol (AP) and 4-nitrophenol (NP). In this communication, they were intraperitoneally administered to male Wistar rats for 7 days and GT activities were determined towards AP, NP, phenolphthalein (PH) and testosterone (TS). Administration of 30 or 20 mg/kg dose of NEN caused marked decrease of liver and body weights, and did not affect hepatic GT activities. Injection of 10 mg/kg dose of NEN did not diminish liver and body weights, and increased the maximally activated GT activities toward AP and NP. In contrast, 30 mg/kg dose of NEA, did not affect either liver and body weights or GT activities. N-Nitrosodimethylamine (NMN), which is a carcinogen and a weak in vitro AP GT activator, was more toxic than NEN, and 3.6 mg/kg dose of NMN appears to induce GT toward NP and AP. Administration of 46.5 mg/kg N-nitrosodibutylamine (NBN), which is a carcinogen but not a GT activator, did not affect GT activities or liver body weights.

Aminophenols↗
PubMed1988

The imbalance in CSF T cell subsets in active multiple sclerosis.

We determined the percentage of each lymphocyte subpopulation in the cerebrospinal fluid (CSF) and the peripheral blood of 7 patients with active multiple sclerosis (MS), 7 with inactive MS, 5 with other inflammatory diseases in the central nervous system, and 12 with non-inflammatory neurological diseases, using fluorescein-labelled monoclonal antibodies (anti-Leu7, anti-HLA-DR, and those that recognize such surface antigens as CD3, CD4, CD8, and CD19), and by laser flow cytometry to clarify the clinical usefulness of their measurement in the assessment of disease activity in MS. In CSF, a significant increase in the percentage of CD4+ cells and a significant decrease in the percentage of CD8+ cells were observed in the active MS group compared with the other 3 groups, while none of the percentages of the 6 subsets studied in the peripheral blood were significantly different among these groups. Our preliminary study indicated that evaluation of the percentages of CD4+ and CD8+ cells in CSF by flow cytometry could be a useful indicator of disease activity in MS.

Adult↗