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Biomedical subjects

M Matsuda

Publications and source records attributed to M Matsuda.

At least 1,189 records · Page 66Linked to original sources
PubMed1985

[Quantitative analysis of myocardial perfusion by digital subtraction angiography].

Digital subtraction angiography (DSA) has been adopted to detect ischemic myocardial areas and to quantitatively evaluate the degrees of myocardial perfusion abnormalities. Subtraction for selective coronary arteriography was performed sequentially between mask and enhanced images in the same cardiac phases by means of ECG signals. We obtained three distinct perfusion phases which we termed the arterial, capillary, and venous phases. Densitometry of the circumferential cardiac wall was performed on these sequential images. The results were compared with 201Tl myocardial scintigrams and cine angiograms. In the arterial phase, the abnormalities of the coronary artery, such as stenosis, obstruction and collaterals were represented. In the capillary phase, contrast materials served as markers of myocardial perfusion and delineated infarctions as areas of low contrast enhancement compared to the normally perfused myocardium. In the venous phase, the infarcted areas were represented as areas with greater relative contrast enhancement than that of the normal myocardium. Circumferential analysis of perfusion could provide more detailed information about myocardial ischemic areas and the degrees of ischemia. Although we used invasive selective coronary arteriography, DSA techniques have numerous merits such as excellent temporal resolution, spatial resolution, and good contrast resolution, over conventional methods. Quantitative DSA methodology which we developed provided not only anatomical information about the main coronary arteries but new information about abnormalities of peripheral myocardial perfusion.

Angiography↗
PubMed1985

[Regional myocardial perfusion abnormality detected by digital subtraction angiography in a case of patent ductus arteriosus with coronary arterial fistulae].

A 19-year-old woman with patent ductus arteriosus and increasingly severe chest pain received various examinations including exercise stress radionuclide test, which revealed hypokinesis and decreased myocardial perfusion in the apex and left ventricular dilatation. Conventional selective coronary arteriography and newly-developed ECG synchronized digital subtraction angiography (DSA) were performed to identify the genesis of her myocardial ischemia. Selective coronary arteriography revealed congenital coronary arterial fistulae in the right and left coronary arteries. The largest fistula was observed around the obtuse marginal artery terminating in the left ventricle. Although stenosis of the left circumflex and left anterior descending arteries (LAD) were not observed, the LAD appeared to be hypoplastic. Hypoplasia of the LAD and the steal phenomenon by arterial fistula could have been responsible for the myocardial ischemia in the apex, but this method did not clearly reveal the area and the degree of myocardial ischemia in the apex. Sequentially subtracted images during the arterial and capillary phases were obtained by utilizing ECG-synchronized DSA for selective coronary arteriography. For these images, densitometric analysis was adapted to the myocardial region, i.e. circumferential densitometry using 21 segments around the left ventricular wall. Decreased myocardial perfusion was clearly demonstrated in the apex in capillary phase images. Moreover, circumferential analysis provided detailed information about the area and the degree of impaired peripheral circulation in the myocardium. This analysis by DSA may prove useful for evaluating myocardial perfusion abnormalities.

Adult↗
PubMed1985

Cellular migration and morphology in corneal endothelial wound repair.

After a mechanical denudation of rabbit corneal endothelial cells, the healing process was followed with wide-field specular microscopy. Individual cell migration and morphologic changes were analyzed by computer-assisted morphometry. The cells surrounding the wound migrated to cover the defect without producing intercellular gaps. The greatest cellular migration and morphologic alterations occurred close to the wound edge. As the cells migrated toward the wound, they elongated and increased their surface area in the direction of the migration. As the healing proceeded, the cells lost their original hexagonal pattern, which returned after coverage was complete. The wound was covered completely by large, irregularly shaped cells showing mitotic figures between 24 and 48 hr. During this period, cellular migration decreased and normal cellular morphology began to recover. When mitosis decreased, the normal cellular pattern rearranged towards a more hexagonal shape. During the healing process, the degree and direction of cellular migration varied from cell to cell. Additionally, changes in cell-to-cell contact (positional changes of neighboring cells) occurred in one-third of migrating cells. Such cellular migration can account for monolayered cells sliding without producing gaps between individual cells.

Animals↗
PubMed1985

A larger corneal epithelial wound closes at a faster rate.

In order to evaluate relationships between healing rates and initial wound area, epithelial wounds were made on rabbit corneas by scraping the epithelium within a 4-, 6.5-, or 8-mm trephine mark. The wounds were stained with fluorescein and photographed during healing. The wounded areas were measured by planimetry. Although larger wounds closed later than smaller wounds, all of the healing curves appeared to be linear. The mean healing rate of the 8-mm diameter wounds (0.91 mm2/hr) was significantly greater than that of the 6.5-mm diameter wounds (0.80 mm2/hr). The 4-mm diameter wounds healed at a significantly slower rate (0.37 mm2/hr) when compared to the 6.5-mm diameter wounds. The authors found a strong positive correlation between the healing rates and the initial wound areas. By comparison, regardless of the initial wound area, the wound diameter decreased at a rate of approximately 0.1 mm/hr, which may explain the dependency of the healing rate on the initial wound area. The healing rate varied considerably between animals with the same diameter wounds, but both eyes of each animal showed a similar healing rate.

Animals↗
PubMed1985

[Clinical observation on in situ carcinoma of the bladder and related conditions].

Clinical course and histological findings of 692 patients with bladder tumor were reviewed to reveal the statistics and clinical profile of carcinoma in situ and its related conditions in the urinary bladder. Primary papillary intraepithelial lesions were found in 83 cases (12.0%), whereas flat intraepithelial carcinoma was found in 16 cases (2.0%), 10 as primary and 6 as secondary tumors. In 35 cases (3.5%), the bladder lesion was flat and rather difficult to find by cystoscopy, and initially considered as chronic cystitis or flat carcinoma in situ. Post-operative histological diagnosis, however, revealed micro- or apparent invasion beyond the basement membrane. Thus they were tentatively classified as clinical carcinoma in situ. The result of the radical surgical treatments for the clinical carcinoma in situ was very poor with a rough 5-year survival of 19%. The management of primary or secondary flat intraepithelial carcinoma was carried out by radical surgery or bladder sparing operation followed by intravesical instillation of cytotoxic agents. The results of these procedures remain uncertain. Some confusion in terminology of carcinoma in situ is discussed and the term "flat carcinoma" was recommended to indicate carcinoma in situ and its related conditions.

Adult↗
PubMed1985

[Do giant negative T waves represent apical hypertrophic cardiomyopathy? Left ventriculographic and cardiac biopsy studies].

Whether giant negative T waves (GNT) of 1 mV or greater represent the electrocardiographic characteristics of idiopathic cardiomyopathy, particularly apical hypertrophic cardiomyopathy, is controversial. To clarify this point, a comparative study was conducted for 57 persons with GNT (GNT+ group) and 57 persons of hypertrophic cardiomyopathy with either asymmetric septal hypertrophy or diffuse hypertrophy of the ventricular wall in the absence of GNT (GNT- group). Patients with cerebral vascular accidents, myocardial infarctions, valvular heart disease, myopericarditis, and implanted pacemakers were excluded from the study because these patients' ECGs may show GNT. Left ventriculograms were investigated at end-diastole in the right anterior oblique projection. The GNT+ group showed a spade-like configuration indicating a marked reduction in the lumen of the apical cavity in 25 cases (43.9%); a kidney or banana-like configuration indicating the inferior wall bulging into the left ventricular cavity in its mid-zone in eight cases (14.0%), and an oval configuration which indicated no deformity of the left ventricular cavity in 24 (42.1%). The spade-like configuration was also seen in four cases (7.0%) of the GNT- group. A study of ventricular wall thicknesses based on left ventriculography showed that 25.5% of cases with GNT did not have concomitant apical hypertrophy. Generally, the GNT+ group cases had a significantly thicker anterior left ventricular wall and apical hypertrophy compared to the GNT- group. The hypertrophic states of the apical wall and anterior wall of the left ventricle correlated well. The GNT+ group included a significantly larger percentage of patients who were middle aged and older than did the GNT- group, and the frequency of accompanying hypertension was significantly higher in the former. The confirmed or suspected familial occurrence of cardiomyopathy (CM) was observed in 32 cases (56.1%) of the GNT- group, and in 18 cases (31.6%) of the GNT+ group, i.e., the incidence was significantly lower in the GNT+ group. In an endomyocardial biopsy study, 40 cases (70.1%) of HCM without GNT had bizarre myocardial hypertrophy with disorganization (BMHD). The incidence of BMHD in the GNT+ group was 21/57 (36.8%)-significantly lower than in the GNT- group. In the GNT- group, there was no significant difference in the incidence of BMHD between relatively young and old patients or between patients with and without accompanying hypertension; whereas, in the GNT+ group, it was significantly lower among those with accompanying hypertension and in older patients than in those without accompanying hypertension and in younger patients.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗