Biomedical subjects

M Matsuda

Publications and source records attributed to M Matsuda.

At least 505 records · Page 28Linked to original sources

PubMed1995

Adhesion of lymphocytes to endothelial cells in experimental allergic encephalomyelitis before and after treatment with endotoxin lipopolysaccharide.

We investigated the in vitro adhesion of 51Cr-labeled lymphocytes to cultured brain endothelial cells and the in vivo expression of intercellular adhesion molecule-1 (ICAM-1) on cerebral endothelial cells in a rat model of experimental allergic encephalomyelitis (EAE) before and after treatment with lipopolysaccharide (LPS). Adhesion of lymphocytes to cerebral endothelial cells was significantly increased in EAE compared with controls (p < 0.01), and was significantly correlated with the percentage of major histocompatibility complex class II antigen-positive cells in lymph node cells (p < 0.001). LPS enhanced ICAM-1 expression on endothelial cells and lymphocyte adhesion to those cells, and caused a significant increase in the in vivo expression of ICAM-1 compared with controls (p < 0.001). Lymphocyte adhesion to endothelial cells was significantly blocked by monoclonal antibodies against ICAM-1, lymphocyte function-associated antigen-1, or very late activation antigen-4. Our findings suggest that lymphocyte adhesion to brain endothelial cells may contribute to lymphocyte migration across the blood-brain barrier in EAE and that LPS may cause progression of EAE lesions.

Animals↗

PubMed1995

The role of fibrinogen D domain intermolecular association sites in the polymerization of fibrin and fibrinogen Tokyo II (gamma 275 Arg-->Cys).

Intermolecular end-to-middle domain pairing between a thrombin-exposed 'A' polymerization site in the central 'E' domain of fibrin, and a constitutive complementary 'a' site in each outer 'D' domain ('D:E'), is necessary but not alone sufficient for normal fibrin assembly, as judged from previous studies of a congenital dysfibrinogen, Tokyo II (gamma 275 arg-->cys), which showed defective fibrin clot assembly and a normal D:E interaction (Matsuda, M., M. Baba, K. Morimoto, and C. Nakamikawa, 1983. J. Clin. Invest. 72:1034-1041). In addition to the 'a' polymerization site, two other constitutive intermolecular association sites on fibrinogen D domains have been defined: between gamma chain regions containing the carboxy-terminal factor XIIIa crosslinking site ('gamma XL:gamma XL'); and between sites located at the outer ends of each molecule ('D:D') (Mosesson, M. W., K. R. Siebenlist, J. F. Hainfeld, and J. S. Wall, manuscript submitted for publication). We evaluated the function of these sites in Tokyo II fibrinogen, and confirmed that there was a normal fibrin D:E interaction, as determined from a normal fibrin crosslinking rate in the presence of factor XIIIa. We also found a normal gamma XL: gamma XL interaction, as assessed by a normal fibrinogen crosslinking rate. Judging from electron microscopic images, factor XIIIa-crosslinked Tokyo II fibrinogen failed to form elongated double-stranded fibrils like normal fibrinogen. Instead, it formed aggregated disordered collections of molecules, with occasional short fibrillar segments. In addition, Tokyo II fibrin formed an abnormal, extensively branched clot network containing many tapered terminating fibers. These findings indicate that the Tokyo II fibrinogen defect results in a functionally abnormal D:D self-association site, and that a normal D:D site interaction is required, in addition to D:E, for normal fibrin or fibrinogen assembly.

Binding Sites↗

PubMed1995

Prevalence of nonthyroid specific autoantibodies in autoimmune thyroid diseases.

To clarify the involvement of polyclonal activation of autoimmune reaction in organ-specific autoimmune diseases, we investigated the prevalence of nonthyroid specific autoantibodies in 50 patients with autoimmune thyroid diseases (22 patients with Graves' disease and 28 with Hashimoto's disease) and in 50 age- and sex-matched controls. None of the 100 subjects had any clinical manifestations or laboratory data indicating any other immunological, infectious, hepatic, or malignant diseases. The prevalence of positive antibodies to nucleus, smooth muscle, and single-stranded DNA in the patients (26%, 36%, and 34%, respectively) was higher than that in the control group (8%, 4%, and 4%, respectively), although neither group was positive for autoantibodies against double-stranded DNA, extractable nuclear antigen, SS-A, SS-B, mitochondria, or rheumatoid factor. Furthermore, 66% of the patients had at least one autoantibody to nucleus, smooth muscle, or single-stranded DNA. In conclusion, patients with autoimmune thyroid diseases show high prevalence of autoantibody against not only thyroid-specific, but also nonthyroid-specific antigens. These results are consistent with the concept that immune reaction of patients with organ-specific autoimmune diseases may be polyclonally accelerated to the production of antibodies against both organ and nonorgan specific autoantigens.

Adult↗

PubMed1995

Soluble E-selectin in the serum and cerebrospinal fluid of patients with multiple sclerosis and human T-lymphotropic virus type 1-associated myelopathy.

To evaluate the activation of endothelial cells of the brain and the spinal cord, we investigated the presence of soluble endothelial leukocyte adhesion molecule-1 (sE-selectin) in the serum and cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) and those with human T-lymphotropic virus type 1-associated myelopathy (HAM). There were significantly higher levels of sE-selectin found in the serum of patients with relapsing-remitting MS during an exacerbation (p < 0.001) and those with chronic progressive MS (p < 0.01) compared with controls. Serum levels of sE-selectin in patients with HAM did not differ significantly from serum levels in controls or non-HAM carriers. We also found sE-selectin in the CSF of eight patients during an exacerbation of relapsing-remitting MS. These results suggest that an active immune reaction involving E-selectin production that is indicative of endothelial cell damage occurs in the CNS of patients during an exacerbation of relapsing-remitting MS. Thus, sE-selectin may be useful in monitoring disease activity in patients with relapsing-remitting MS.

Adult↗

PubMed1995

Bordetella bronchiseptica dermonecrotizing toxin stimulates assembly of actin stress fibers and focal adhesions by modifying the small GTP-binding protein rho.

We studied the biochemical mechanism of morphological changes in cells treated with Bordetella dermonecrotizing toxin (DNT). DNT caused the morphological changes of serum-starved MC3T3-E1 cells from flat shapes to reflactile ones. These changes were accompanied by the assembly of actin stress fibers and focal adhesions, which is known to be regulated by the small GTP-binding protein rho. Clostridium botulinum C3 exoenzyme, which ADP-ribosylates and inactivates rho protein, 'rounded' the cells within 2 hours after addition to the extracellular fluid and their rounded shapes were maintained for at least 10 hours. However, when the cells were co-treated with C3 exoenzyme and DNT, they were rounded at 2 hours but recovered an apparently intact morphology after 3-8 hours of incubation. rho proteins in lysates from DNT-treated cells and untreated cells were radiolabeled by [32P]ADP-ribosylation with C3 exoenzyme and analyzed by SDS-polyacrylamide gel electrophoresis. Whereas the lysate from untreated cells showed a single band of [32P]ADP-ribosylated rho protein, the lysate from DNT-treated cells showed an additional two bands as well as the band identical to that of the lysate from untreated cells. Recombinant rhoA protein treated with DNT in vitro also showed a mobility shift in SDS-polyacrylamide gel electrophoresis. These results indicate that DNT causes the assembly of actin stress fibers and focal adhesions by directly modifying rho protein.

ADP Ribose Transferases↗

PubMed1995

Axial rotation in rat embryos: involvement of changes in the shapes and arrangement of cells.

Rat embryos at the head-fold stage (9.5 days of gestation) were cultured for 32 hours in rat serum. Embryos rotated their axes (changing from the shape of a concave mid-region to that of a convex mid-region) during the last 5 hours of culture (from 27 h to 32 h in culture). Addition of 0.1 micrograms/ml cytochalasin D to the culture medium for this 5-hour period prevented axial rotation in the embryos and disturbed the appearance of microfilaments in the dermatome, the dorsal region of the trunk neural tube, and the dorsal epidermis. During the period of axial rotation, the dermatome and the dorsal epidermis extended and showed the arrangement of microfilaments along the cranio-caudal axis in the control embryos but not in the treated embryos. The dorsal region of the trunk neural tube in the control embryos consisted of a seam of neuroepithelial cells in which microfilaments were apparently arranged along the cranio-caudal axis but the region in the treated embryos was crowded with the neuroepithelial cells piled up randomly and microfilaments showed no arrangement. These results suggest that changes in the shapes and arrangement of the cells in the dermatome, the dorsal region of the trunk neural tube, and the dorsal epidermis cause extension of these tissues along the cranio-caudal axis and result in axial rotation. Microfilaments may play an essential role in changes in the shapes and arrangement of the cells in these tissues.

Animals↗

PubMed1995

Sperm morphology and IMP distribution in membranes of spermatozoa of cyprinid fishes II.

The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of comparative spermatology in cyprinid fishes and was continued from a previous paper (Ohta et al., 1994 (8)). Although the fundamental structures of the spermatozoa were similar among the four species, the distribution of IMPs varied. Hexagonal (or parallelogrammic) arrays of IMPs on the sperm head membranes were present in G. elongatus elongatus, A. lanceolatus and P. tetrazona, but not in P. esocinus. The hexagonal arrays have now been confirmed to be present in twelve of the thirteen species of cyprinid fishes examined so far.

Animals↗

PubMed1995

Studies on pyridonecarboxylic acids. IV. Synthesis and antibacterial activity evaluation of S-(-)- and R-(+)-6-fluoro-1-methyl-4-oxo-7- (1-piperazinyl)-4H-[1,3] thiazeto[3,2-a]quinoline-3-carboxylic acids.

Optically active isomers of 6-fluoro-1-methyl-4-oxo-7-(1-piperazinyl)-4H- [1,3] thiazeto [3,2-alpha] quinoline-3- carboxylic acid (NM394, 3) were prepared through optical resolution of their racemic intermediate ( +/- )-1 by high-performance liquid chromatography (HPLC). The absolute configuration at the C-1 position in the thiazeto-quinolone ring of ( - )-3 was confirmed by X-ray analysis ( - )-4 to be S. The in vitro antibacterial activity of ( - )-3 was 2--8 times that of (+)-3.

Anti-Infective Agents↗

PubMed1995

Studies on pyridonecarboxylic acids. III. Synthesis and antibacterial activity evaluation of 1,8-disubstituted 6-fluoro-4-oxo-7-piperazinyl-4H-[1,3]thiazeto[3,2- a]quinoline-3-carboxylic acid derivatives.

A series of 1,8-disubstituted 6-fluoro-4-oxo-7-piperazinyl-4H-[1,3]thiazeto[3,2-a]quinoline-3- carboxylic acid derivatives was prepared and evaluated for antibacterial activity. In the 7-piperazinyl series, addition of a fluorine at C-8, which increased the in vitro activity for the 1-hydrogen and 1-methyl analogues and decreased it for the 1-phenyl analogue, improved the in vivo activity of all the analogues. Introduction of a methoxy group at C-8 of the 1-methyl-7-piperazinyl analogue also improved its in vivo antibacterial activity. The effect of 8-substituents on the in vitro and in vivo antibacterial activity of the 1-methyl-7-(4-methyl-1-piperazinyl) series is also discussed.

Animals↗

PubMed1995

The phylogeny of Yamadazyma ohmeri (Etchells et Bell) Billon-Grand based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Kodamaea gen. nov. (Saccharomycetaceae).

Two strains of Yamadazyma ohmeri were examined for their 18S and 26S rRNA partial base sequences. The two strains had identical or very similar partial base sequences to those of the type strain. All the three strains of Y. ohmeri were phylogenetically separated from any other accepted species of the genera Yamadazyma, Debaryomyces, Pichia, Williopsis, Metschnikowia, and Clavispora. Based on the sequence data obtained and the phenotypic characteristics, Kodamaea gen. nov. was proposed for the species.

Molecular Sequence Data↗

PubMed1995

The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae).

Twelve strains of methanol-assimilating yeast species were examined for their partial base sequences of 18S and 26S rRNAs. In the partial base sequencings of 18S rRNA (positions 1451-1618, 168 bases), P. kodamae had the same partial base sequence as Ogataea minuta, and P. finlandica, P. pini, P. trehalophila, C. maris, and C. methanolovescens were the same in the partial base sequences as O. glucozyma. Candida boidinii and C. methylica were similar to O. glucozyma, but somewhat different from O. minuta (the base differences, one and four, respectively). Pichia naganishii had seven, four, six, and nine base differences with O. minuta, O. glucozyma, P. anomala, and P. membranaefaciens, respectively, and C. methanosorbosa did four, three, five, and twelve base differences. Pichia pastoris was quite different (the base differences among the methanol-assimilating yeasts, 32-31), and no bases were found in the fingerprint segment. In the partial base sequencings of 26S rRNA (positions 1611-1835, 225 bases; positions 493-622, 130 bases), P. finlandica, P. kodamae, P. pini, P. trehalophila, C. maris, C. methanolovescens, and C. methanosorbosa were similar to O. minuta and O. glucozyma. Pichia naganishii, C. boidinii, and C. methylica had somewhat different partial base sequences. The base differences and the percent similarities of P. pastoris were quite high (101-89) and quite low (40-47). Based on the sequence data obtained, the methanol-assimilating yeast species are discussed taxonomically and phylogenetically. A new genus, Komagataella was proposed for P. pastoris with a new combination, Komagataella pastoris.

Base Sequence↗

PubMed1995

The phylogenetic relationships of the Q9-equipped, hat-shaped ascospore-forming species of the genus Yamadazyma Billon-Grand (Saccharomycetaceae) based on the partial sequences of 18S and 26S ribosomal RNAs.

Sixteen strains of the sixteen species, including the type species, Y. philogaea (CBS 6696, type strain), of the genus Yamadazyma were examined for their partial base sequences of 18S and 26S rRNAs. The genus Yamadazyma Billon-Grand was found to have a heterogeneous nature phylogenetically. In the partial base sequences in positions 1451-1618 (168 bases) of 18S rRNA, the number of base differences was 4-0 within the genus except for Y. spartinae, Y. inositovora, Y. ohmeri, and Y. besseyi. The base differences numbered 6-1, 11-8, and 8-4 with D. hansenii, P. membranaefaciens, and S. cerevisiae, respectively. In the partial base sequences in positions 1611-1835 (225 bases) of 26S rRNA, the number of base differences was 14-0 within the genus. The base differences numbered 19-0, 31-24, and 25-17 with D. hansenii, P. membranaefaciens, and S. cerevisiae, respectively. In the partial base sequences in positions 493-622 (130 bases) of 26S rRNA, the percent similarities were 73-93. The percent similarities were 77-90, 64-71, and 68-79 with D. hansenii, P. membranaefaciens, and S. cerevisiae, respectively. Yamadazyma inositovora, Y. spartinae, and Y. ohmeri were not closely related phylogenetically. Yamadazyma besseyi (Q-7) was separate phylogenetically from the species mentioned above of the genera Yamadazyma, Debaryomyces, Pichia, and Saccharomyces (base differences, 13-7 and 62-17; percent similarities, 48-63). The discussion was made phylogenetically and taxonomically, especially on transferring Y. besseyi to a separate taxon.

Base Sequence↗

PubMed1995

The phylogenetic relationships of Pichia jadinii, formerly classified in the genus Hansenula, and related species based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae).

We analyzed 18S and 26S rRNA partial base sequences [positions 1451-1618 (168 bases) of 18S rRNA and positions 1611-1835 (225 bases) and 493-622 (130 bases) of 26S rRNA] of a total of three strains of Pichia jadinii and Candida utilis. The three strains had identical base sequences with the type strain of P. jadinii (IFO 0987) in the 18S rRNA partial base sequencings. In the 26S rRNA partial base sequencings, there were partial base sequences similar to each other (1-0 base difference and 87-95 percent similarities). The sequence data obtained are discussed taxonomically and phylogenetically, especially in connection with Williopsis saturnus, the type species of the genus Williopsis Zender.

Base Sequence↗

PubMed1995

The phylogeny of Williopsis salicorniae Hinzelin, Kurtzman et Smith based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae)

Williopsis salicorniae IFO 10733 (type strain), which is characterized by the formation of saturn-shaped ascospores, by the incapability of assimilating nitrate, and by a lower DNA base composition (36.7 mol% G + C), was examined for its partial base sequences of 18S and 26S rRNAs. In the 18S rRNA partial base sequencings, it had an identical base sequence with the type strain of Ogataea glucozyma (identical to Pichia glucozyma, identical to Hansenula glucozyma), which produces hat-shaped ascospores and has the ability to assimilate nitrate and methanol and a higher DNA base composition (45.1 mol% G + C). In the 26S rRNA partial base sequencings, the base differences were four, and the percent similarity was 87 between the type strains of the two species. The data obtained are discussed phylogenetically and taxonomically.

Base Sequence↗

PubMed1995

Modification of pancreatic digestive function by pituitary grafting in mice.

The effect of prolactin on the digestive potency of the acinar pancreas was examined in pituitary-grafted hyperprolactinemic mice, because our previous experiment showed that a marked proliferation of pancreatic acinar cells was induced by pituitary grafting in mice. To know whether the digestive function is modified, the tissue contents of pancreatic digestive enzymes, such as chymotrypsin, lipase alpha-amylase and ribonuclease, were measured in the hyperprolactinemic mice. Pituitary grafting significantly increased the contents of chymotrypsin and lipase in the pancreas on day 12 after the operation without affecting intake of food, when compared to those in the sham-operated controls. On day 30, however, the differences between pituitary-grafted and control mice were no more discernible. Thus, the digestive enzyme activities are easily modified soon after the increase of circulating prolactin level. This effect of prolactin on the function of the pancreas may be responsible for "homeorhetic" control of nutrients during lactation. In another set of experiments in adrenalectomized-castrated or castrated mice, pituitary grafting induced an increase in the weight of the pancreas. In addition, adrenalectomy in combination with castration did not alter the pancreatic contents of chymotrypsin and lipase but decreased the amylase content. These results taken together seem to indicate that the effect of prolactin on the exocrine pancreas is not mediated by gonadal and adrenal steroid hormones.

Adrenalectomy↗

PubMed1995

Cytoplasmic body myopathy with hypertrophic cardiomyopathy.

A patient with cytoplasmic body myopathy presented muscle hypotonia from birth and developed progressive muscular atrophy and weakness, scoliosis, contracture of joints and cardiorespiratory failure. At the age of 17, he died of heart failure. Post mortem examination revealed severe hypertrophy of cardiac walls and generalized muscular atrophy. Microscopic examination showed many cytoplasmic bodies in skeletal muscle fibers and myofiber disarray in myocardium. No cases of cytoplasmic body myopathy with hypertrophic cardiomyopathy have been reported previously. It is suggested that the Z-line component is related to the formation of the cytoplasmic body in skeletal muscle and disarray in the cardiac muscle.

Adolescent↗

PubMed1995

Enlargement of multiple cavernous hemangioma of the liver in association with pregnancy.

Four cavernous hemangiomas were found in a 34-year-old woman after the first delivery. All four hemangiomas became enlarged after the second delivery. Due to complaints of symptoms of compression, and to rule out malignancy, surgical intervention was employed. Histologically, the tumors were typical cavernous hemangiomas. Although a relationship between enlargement of hemangiomas and estrogen was suggested, estrogen receptors were not detected in the tumors.

Adult↗

PubMed1995

Effects of angiotensin-converting enzyme inhibitors, Ca2+ channel antagonists, and alpha-adrenergic blockers on glucose and lipid metabolism in NIDDM patients with hypertension.

We compared the effects of captopril, nifedipine, and doxazosin on glucose and lipid metabolism in 30 hypertensive non-insulin-dependent diabetes mellitus (NIDDM) patients (age = 50 +/- 3 years; body mass index = 30 +/- 1 kg/m2). Of these patients, 9 were treated with captopril, 11 with nifedipine, and 10 with doxazosin for 12 weeks. Blood pressure, fasting plasma glucose (FPG) concentration, HbA1c, oral glucose tolerance test (OGTT), euglycemic insulin clamp, and plasma lipids were measured before and after a 3-month period. Mean arterial blood pressure (114 +/- 2 mmHg) was similar in all groups before initiating antihypertensive therapy and declined to 102 +/- 2 (captopril), 103 +/- 1 (nifedipine), and 103 +/- 2 (doxazosin) mmHg (P < 0.001). Baseline FPG (148 +/- 11 mg/dl) and HbA1c (6.3 +/- 1%) were similar in all groups and did not change significantly with treatment. Plasma glucose, insulin, and free fatty acid (FFA) concentrations during the OGTT were similar in all groups before antihypertensive treatment and did not change with captopril and nifedipine; after doxazosin, plasma glucose and FFA concentrations during the OGTT decreased (both P < 0.05) without change in plasma insulin response. Insulin-mediated glucose uptake (144 +/- 11 mg.m-2.min-1), glucose oxidation (76 +/- 4 mg.m-2.min-1), and nonoxidative glucose disposal (71 +/- 6 mg.m-2.min-1) were similar in all groups before the start of antihypertensive treatment and did not change in captopril and nifedipine groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenergic alpha-Antagonists↗