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Biomedical subjects

M Marotta

Publications and source records attributed to M Marotta.

26 records · Page 2Linked to original sources
PubMed1984

[Ultrastructure of human cerebral neoplasms in vitro: medulloblastoma].

The fine structure of five lines of medulloblastoma cells in long-term cell cultures are described. The cell cultures were fixed in situ by the rapid exchange of the medium for the fixative solution consisting of 3% glutaraldhyde in 0.1 M Na cacodylate-HC1 buffer with 0.1 M sucrose (pH 7,2, total osmolality 510 mOsm, vehicle osmolality 300 mOsm). The cultures were subsequently postfixed in 1% OsO4 in water at room temperature and dehydrated in a graded series of ethanol solutions. In the second change of 100% ethanol they were stained with 2% uranyl acetate for 10 minutes. They described the structural characteristics of these cells in relation to the various phases of the culture, is similar to the well known of in vitro cellular embryonal systems (spongioblasts).

Adolescent↗
PubMed1980

[Nomarski interference microscopy: use of combined contrast uranyl acetate-PTA for in vitro observation of monolayer cultures].

In this paper is reported a technique for the fixation and staining of cellular monolayers in vitro for observation by the interferential microscope according to Nomarski. This method includes the use of glutaraldehyde at 2.5% in phosphate buffer 0.1 M pH 7.5 and the sequential use of Uranil acetate (0.9% in absolute alcohol) and phosphtungstic acid (1% in absolute alcohol). This study has been conducted on 3 cellular lines: PC12 (rat pheocromocytoma), R.P.C. (rat pineal cells), primary culture obtained from a human carcinoma of the uterus.

Acetates↗
PubMed1980

[Practical staining method: T-OPA triple stain].

The following procedure has proven to be successful as routine trichrome stain on paraffin embedded material: 1) Mayer's hemalum for 10 min, followed by running tap water wash; 2) staining in 1% Orange G in 1% acqueous PTA for 5 min and rinsing a few seconds in distilled water; 3) Aniline blue 1% acqueous for 5 min, followed by few seconds distilled water wash. Dehidratation in ethanol, or by blotting followed by t-buthanol or 1:3 terpineol-xylene, clearing and mounting, completed the procedure.

Acridine Orange↗
PubMedDate not supplied

[Muscle regeneration following glycerol injection mimic that of mdx-mice degenerative-regenerative groups].

INTRODUCTION: In recent years several authors have proposed that muscle pathological changes in humans with dystrophin disorders and in mdx-mice are the result of regeneration attempts of this tissue subjected to constantly repeated bouts of necrosis. AIM: We compared morphological aspects of degenerative-regenerative groups observed in the mdx-mice with the regenerative phases that follow an intramuscular injection of 30 mL glycerol. MATERIALS AND METHODS: Gastrocnemius samples from mdx-mice, 1 to 5 months old and from glycerol injected C57Bl10/ScSn mice, 4 to 8 months old, were processed and analyzed in parallel. RESULTS: We were able to recognize degenerative-regenerative group stages in the mdx-mice that closely matched the anomalies found 1, 2, 3, 4-5, 7 and 21-28 days following the injection with glycerol. CONCLUSION. This study reinforces the hypothesis that muscle pathologic abnormalities of mdx-mice are the result of chronic muscle necrosis-regeneration. Morphologic mdx-mice degenerative-regenerative group staging will be a helpful tool for future investigation of muscle regeneration.

Animals↗