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PubMed · 6161625

[Practical staining method: T-OPA triple stain].

G Tajana·M Marotta·L De Palma
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Abstract

The following procedure has proven to be successful as routine trichrome stain on paraffin embedded material: 1) Mayer's hemalum for 10 min, followed by running tap water wash; 2) staining in 1% Orange G in 1% acqueous PTA for 5 min and rinsing a few seconds in distilled water; 3) Aniline blue 1% acqueous for 5 min, followed by few seconds distilled water wash. Dehidratation in ethanol, or by blotting followed by t-buthanol or 1:3 terpineol-xylene, clearing and mounting, completed the procedure.

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Acridine OrangeAniline CompoundsPhosphotungstic AcidStaining and LabelingEnglish AbstractJournal Article

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G Tajana, M Marotta, L De Palma. 1980-10-15. [Practical staining method: T-OPA triple stain].. https://pubmed.ncbi.nlm.nih.gov/6161625/

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