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Biomedical subjects

M Maeda

Publications and source records attributed to M Maeda.

At least 1,171 records · Page 65Linked to original sources
PubMed1987

Hypouricemia due to subtotal defect in the urate transport.

Hypouricemia due to renal tubular defects is rarely reported. We report here our observations of two such patients who cannot be classified into any of the previously reported categories of renal hypouricemia. They showed no suppression of urate clearance to creatinine clearance ratio (Cua/Ccr) following the administration of pyrazinamide, and no increase of Cua/Ccr after probenecid. It is tempting to speculate upon the subtotal defect in the urate transport as a condition explaining the above results.

Adult↗
PubMed1987

[Cytotoxicity of T-lymphocytes from a patient with choriocarcinoma to autologous tumor cultured cells--stimulation with the husband's lymphocytes].

The authors recently studied the histocompatibility between patients with choriocarcinoma and their husbands and did an in vitro experiment (in an autosystem) to establish whether choriocarcinoma can be treated with lymphocytes from the husband. The results were as follows. Seventeen pairs of trophoblastic diseases were analyzed for HLA antigens. The histocompatibility rate between the patient and husband was 60% in the choriocarcinoma group, which was larger than the rates for both the hydatidiform mole group (29%) and the invasive mole group (0%). We succeeded in establishing a cultured cell strain of choriocarcinoma. We named this strain SMT-cc 1 and used it in the subsequent autologous experiments. Peripheral T-lymphocytes of the patient with SMT-cc 1 were examined for their cytotoxicity against SMT-cc 1 using the 51Cr release assay. T-lymphocytes of the patient showed a cytotoxicity of 7.6% after independent culture (control experiment), while they showed a cytotoxicity of 19.7% after a 6-day mixed culture with the husband's lymphocytes. The latter figure was almost equal to the rate (22.2%) observed for T-lymphocytes after a similar stimulation procedure using SMT-cc 1. When anti-HLA-A, B antibody was added during the above mentioned cytotoxicity test, cytotoxicity was markedly reinforced. The results of this study indicate the effectiveness of specific immunotherapy which, making use of the fact that choriocarcinoma expresses the husband's genome, intravenously injects lymphocytes into the patient, after stimulation with antigens (husband's lymphocytes or choriocarcinoma cells).

Antibody-Dependent Cell Cytotoxicity↗
PubMed1987

Evaluation of peripheral vascular disease by intravenous digital subtraction angiography with the Fuji computed radiography (FCR) system.

Intravenous digital subtraction angiography using Fuji computed radiography system (FCR-DSA) was used to evaluate 57 preoperative patients with clinical manifestations of peripheral vascular disease of lower extremity. With its high contrast resolution and good special resolution, image of FCR-DSA could offer optimal information of vascular abnormality in lower extremity distal to midfoot for the diagnosis and the choice of management.

Adult↗
PubMed1987

[A simple and rapid crystal violet uptake sensitivity test for anticancer agents].

Hitherto, the selection of anticancer drug has been based on clinical experience. But to succeed for chemotherapy to succeed, effective drugs must be selected for individual patients with cancer. We have developed the following new in vitro micromethod. Method. In brief, tumor cells obtained by surgery were incubated with anticancer drugs for 48 hrs at 37 degrees C in 5% CO2 in a microplate. After incubation, the surviving cells were fixed with methanol and stained with crystal violet. Then the stained cells were solubilized with 1% lauryl sulfate and the absorbance of each well was measured at 540 nm with a multiscan spectrophotometer. In this method, cytotoxicity was quantitated by the absorbance. The method is simple, rapid (48 hrs) and reproducible, and it requires only a small amount of cells (5 X 10(3) approximately 1 X 10(4]. This method correlates well with sensitivity tests using isotopes. We have examined the chemosensitivity of 22 specimens from gynecologic malignancies by this method. The success rate is 77% and higher than with any other in vitro method. This method will be widely utilized in several fields of cancer treatment in the near future.

Antineoplastic Agents↗