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Biomedical subjects

M M Ferguson

Publications and source records attributed to M M Ferguson.

At least 127 records · Page 7Linked to original sources
PubMed1983

Progesterone metabolism in the murine submandibular salivary gland.

Homogenates of submandibular salivary glands from female mice were incubated with progesterone. This resulted in the formation of 20 alpha-hydroxy-progesterone, thus indicating the presence of the enzyme, 20 alpha-hydroxysteroid dehydrogenase.

20-Hydroxysteroid Dehydrogenases↗
PubMed1983

The anatomy of the facial nerve in relation to CT/sialography of the parotid gland.

The technique of computed tomography with simultaneous parotid sialography allows for accurate localisation of tumours within the parotid gland. The relationship of these tumours to the facial nerve, which divides within the gland, is of surgical significance. A study of the course of the facial nerve through the parotid gland in the cadaver was therefore undertaken in order to identify relevant landmarks for the CT prediction of its relationship to those tumours. The heads of five cadavers were sectioned in the Frankfurt plane into 1 cm slices which were then X-rayed. Individual slices were examined and the course of the facial nerve was plotted on the X-ray plate of the same slice. It was found that the nerve could be represented on a CT scan by an arc of radius 8.5 mm, the centre of which is the most posterior point of the ramus of the mandible. The arc extends from directly lateral to directly posterior to that point. Pre-operative visualisation of parotid tumours by simultaneous CT scan and sialography in conjunction with an appreciation of the course of the facial nerve through the parotid gland may allow for more precise presurgical planning.

Facial Nerve↗
PubMed1983

Accumulation of thiourylene antithyroid drugs in mouse salivary gland.

[35S]Methimazole and [35S]propylthiouracil were shown to accumulate in mouse submandibular gland in vivo, with maximal tissue: plasma ratios being achieved at the lowest dose of drug studied (0.1 microgram/animal). Autoradiography of submandibular glands showed that the drugs were localized to the intralobular ductal epithelium and within the lumen of the convoluted granular tubule, which was identical to the localization of radiolabelled iodide. Histochemical studies indicated that this was the site of peroxidase activity within the gland. Drug accumulation persisted when iodide trapping was competitively inhibited using perchlorate. These data suggest that antithyroid drug accumulation by this tissue is not dependent on the anion trap; the localization of drug and iodide at the site of peroxidase activity suggest that this may be an important factor in the mechanism of drug accumulation, possibly related to subsequent drug metabolism.

Animals↗
PubMed1983

Influence of sodium perchlorate on thiourylene antithyroid drug accumulation in mice.

Radiolabelled [35S]propylthiouracil and [35S]methimazole were shown to accumulate in mouse thyroid gland in vivo, with maximal tissue/plasma ratios and maximal intrathyroidal levels of 35S-labelled drug being seen at the lowest dose of drug studied (0.1 micrograms/animal). Pretreatment with sodium perchlorate (10 mg) abolished iodide trapping by the thyroid and caused a fall in accumulation of both [35S]methimazole and [35S]propylthiouracil, although this effect was not seen at higher doses of drug, when tissue/plasma ratios approached unity. These data suggest that thiourylene antithyroid drug accumulation by the thyroid gland does not depend directly on the anion trap, and it is suggested that this accumulation might depend on subsequent intrathyroidal drug metabolism.

Animals↗
PubMed1982

A quantitative study on growth and cell population identification in murine salivary gland culture.

Differentiation of neonatal salivary gland cell populations was studied in vitro using primary explant cultures. Growth on glass of the submandibular, sublingual and parotid salivary glands was optimal if the initial explant diameter was less than 0.4 mm and culture was in 199 medium plus 20 per cent newborn-calf serum. Growth was measured by planimetry of the area of cellular outgrowth as a monolayer with continuous cell contacts. Duct epithelial cells identified using ultrastructural, histochemical and immunological criteria, produced evidence of altered morphology by in-vitro conditions. Preservation of enzymic and antigenic markers of ductal epithelial cell origin in vitro irrespective of gross cell morphology allowed quantification of these cells in a mixed cell population.

Animals↗
PubMed1982

Crohn's disease of the mouth: an indicator of intestinal involvement.

Nineteen patients with clinical evidence of oral Crohn's disease but no intestinal symptoms were studied. Oral lesions in all patients were shown histologically to have lymphoedema with or without chronic granulomas consistent with Crohn's disease. Seven patients (37%) had demonstrable intestinal disease on rectal biopsy and four of these had abnormal bowel radiology. All seven had evidence of nutritional deficiency. Patients with clinical features suggesting oral Crohn's disease may have evidence of Crohn's disease in the intestine, although this may not be clinically apparent.

Adolescent↗
PubMed1981

Oral complaints related to climacteric symptoms in oöphorectomized women.

Numerous oral complaints have been attributed to the female climacteric including altered taste, a burning sensation and xerostomia. However, the data relating these symptoms to the climacteric require elucidation as there have been no adequately controlled studies. In the present investigation, a group of 145 oöphorectomized women were followed for one year. Approximately half were treated with oestrogen replacement and the remainder with a placebo. The results indicate that the hormone had no direct effect upon the oral symptoms but that there was a general increase in somatic complaints which appeared to be related to the degree of neurosis experienced. This is turn can be attributed to vasomotor changes which are under the control of oestrogen.

Adult↗
PubMed1980

Coeliac disease associated with recurrent aphthae.

Fifty patients presenting sequentially with a history of recurrent aphthae were investigated for evidence of nutritional deficiencies and coeliac disease. In the group, two patients were found to have coeliac disease and their recurrent aphthae cleared soon after starting a gluten free diet. This study confirms the presence of an increased prevalence of nutritional deficiency and of coeliac disease in aphthous patients. However, it is recommended that jejunal biopsy be carried out in these cases only where there is evidence of malabsorption.

Adolescent↗