Search PubMed⌕ Search

Biomedical subjects

M Kuroda

Publications and source records attributed to M Kuroda.

At least 487 records · Page 27Linked to original sources
PubMed1981

Total retropubic prostatectomy for carcinoma of the prostate.

Total prostatectomy is an effective mode of therapy for early carcinoma of the prostate. This report deals with 74 patients who underwent total retropubic prostatectomy at our hospitals during a 23-year period. The 5-year survival rates of patients with stages A and B, C, and D carcinomas were 84.1, 40.1, and 12.7% respectively. The total 5-year survival rate was 56.1%. The 10-year survival rates compare favorably with those of any other mode of therapy. The histological grade was highly significant with respect to prognosis. Combined orchiectomy with total prostatectomy was effective. Operative mortality was 4.05% (3 patients), and the main complication was incontinence. We strongly believe that total prostatectomy is the only curative treatment for early carcinoma of the prostate. This procedure should be utilized more frequently.

Aged↗
PubMed1981

Bilateral congenital multicystic dysplasia of the kidney.

The clinical and pathological features of congenital multicystic dysplasia of the kidney are reviewed and we report of a patient with bilateral dysplasia who lived longer than do most such patients. When multicystic disease of the kidney is bilateral, it is commonly confused with polycystic disease. Despite the fatal prognosis, differentiation is essential for appropriate genetic counselling since multicystic disease is not a familial occurrence.

Humans↗
PubMed1981

Eu-actinin, a new structural protein of the Z-line of striated muscles.

A new protein component of the Z-line of striated muscles was isolated from chicken breast muscle. This protein has been designated as eu-actinin because of its close similarity in polypeptide molecular weight to actin. Eu-actinin was extracted from myosin-removed myofibrils at low ionic strength at pH 6.5 and purified by column chromatography on Sepharose 4B and DEAE-cellulose. Although the polypeptide molecular weight of eu-actinin measured by SDS-polyacrylamide gel electrophoresis is similar to that of actin, other physico-chemical properties of eu-actinin definitely differ from those of actin. The isoelectric point of eu-actinin was more acidic than that of actin. The amino acid composition of eu-actinin was found to be different from that of actin or those of other muscle structural proteins. The results of analytical gel filtration on Sepharose 4B indicated that eu-actinin forms dimers through non-covalent bonding under aqueous conditions. Eu-actinin has a low axial asymmetry under low-salt conditions, as judged from its intrinsic viscosity ([eta] = 6.4 ml/g for the dimer state) and exhibits a tendency to undergo self-association with increasing ionic strength. Interactions of eu-actinin with other muscle proteins were examined by the affinity column technique. It was shown that eu-actinin binds to actin and alpha-actinin. Eu-actinin exhibited strong seeding ability for the polymerization of actin. Antibody to eu-actinin was raised in a goat and purified by affinity chromatography. The specific antibody against eu-actinin did not form precipitine lines with actin or alpha-actinin. Immunofluorescence studies revealed that eu-actinin is localized at the Z-line of myofibrils. The FITC-conjugated antibody to eu-actinin also stained the Z-lines of rabbit skeletal muscle and chicken cardiac muscle. Therefore, it was concluded that eu-actinin is a new, ubiquitous constituent of Z-lines of striated muscles.

Actinin↗
PubMed1981

Successful treatment of fulminating complications associated with extensive rhabdomyolysis by plasma exchange.

The first successful treatment by plasma exchange of acute renal failure and disseminated intravascular coagulation, as well as liver and central nervous system damage, was achieved in two patients with severe multiorgan damage associated with extensive muscle tissue damage caused by heatstroke and/or electrical injury. Substances derived from damaged muscle tissue, such as myoglobin and enzymes, were efficiently removed by a cellulose acetate membrane filter. Improvement of consciousness, disturbance, coagulation disorder, and renal failure were observed. The disorders associated with extensive rhabdomyolysis seem to be a promising indication for this therapy. Plasma exchange with a membrane filter is useful for treatment of patients with renal failure, being readily connected on-line with conventional dialysis.

Acute Kidney Injury↗
PubMed1980

Extractability of actin and actin-like protein from myosin-removed myofibrils of skeletal muscle.

Extractability of 42,000 dalton components (42 K component) from myosin-removed myofibrils (called I-Z-I segments in this paper) under low-salt conditions was compared at pH 6.5 and pH 8.0. Kinetic and isoelectric focusing analysis of the 42 K components revealed that the 42 K component extracted at pH 8.0 was actin, which depolymerized from the end of the I-filaments of the I-Z-I segments. On the other hand, isoelectric focusing of the 42 K component extracted at pH 6.5 indicated two protein bands. The relative mobility of the minor band was virtually identical to that of actin. While the isoelectric point of the major band was more acidic than that of skeletal muscle actin. Therefore, it is concluded that there are at least two kinds of 42,000 dalton components in the myosin-removed myofibrils.

Actins↗
PubMed1980

The first cases of Fitzgerald factor deficiency in the Orient: three cases in one family.

Asymptomatic, female, 56-year-old identical Japanese twins were found to have a severe abnormality in the surface-mediated intrinsic coagulation, fibrinolysis and esterolytic activity. These defects were thought to be due to the lack of Fitzgerald factor, because of the prolongations of kaolin-activated partial thromboplastin time and kaolin-activated euglobulin lysis time that were not corrected by the addition of Fitzgerald trait-plasma but were corrected to normal levels by the addition of isolated bovine high-molecular-weight kininogen, Fletcher trait-plasma or Hageman trait-plasma.

Animals↗