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Biomedical subjects

M Kuroda

Publications and source records attributed to M Kuroda.

At least 361 records · Page 20Linked to original sources
PubMed1990

[Mitral valve prolapse syndrome as an etiologic factor of anxiety disorder].

To assess whether a certain somatic disease is involved in the etiology of anxiety disorder, we examined the relationship between anxiety disorder and mitral valve prolapse syndrome (MVP). Patients with anxiety disorder were diagnosed according to the criteria of the American Psychiatric Association (Quick Reference to the Diagnostic Criteria from DSM-III). Two-dimensional echocardiography was carried out on 36 normal controls (15 males and 21 females) and 39 patients with anxiety disorder (19 males and 20 females); including 21 patients with panic disorder, 12 with generalized anxiety disorder, and 6 with atypical anxiety disorder. The echocardiograms were evaluated by a cardiologist unaware of clinical background about these patients. The presence of MVP was diagnosed according to the criteria by Yoshikawa, et al. or Nagata, et al. Findings of MVP were seen in 18 (46.1%) of the patients and 5 (13.9%) of the controls indicating a significantly higher incidence (chi 2 = 7.711, p less than 0.01) in the patients. In the patient subgroups, 14 patients with panic disorder (66.6%) and 4 with generalized anxiety disorder (33.3%) had MVP, showing a significantly higher incidence in the former (chi 2 = 14.335, p less than 0.01) than in the normal controls, but no statistical significance in the latter. No MVP was found in the patients with atypical anxiety disorder. These results suggest that some somatic diseases such as MVP may play a role in the etiology of anxiety disorder, especially panic disorder.

Adult↗
PubMed1990

[A case of synchronous multiple primary cancers of the stomach and kidney].

In line with an increase in the incidence of multiple primary cancers, we have encountered a case of synchronous multiple primary cancers of the stomach and the left kidney. The patient, a 69-year-old male, visited our hospital after experiencing epigastric discomfort for three months. An advanced gastric cancer, Borrmann III type, was detected by endoscopic examination. Preoperative abdominal computed tomography also revealed a large low density mass occupying the upper part of the left kidney. On angiography, the left kidney showed a hypervascular mass, showing pooling and tumoral stains, thereby suggesting a renal cell carcinoma. The patient thus underwent a subtotal gastrectomy with an R2 lymph node dissection and a left radical nephrectomy. Histologically, the gastric lesion was a poorly-differentiated adenocarcinoma and the left renal lesion was a renal cell carcinoma of the clear cell type.

Adenocarcinoma↗
PubMed1990

[Group specific component protein (Gc) in patients with acute hepatic necrosis].

We studied the clinical significance of serum Gc levels in the sera of patients with acute hepatic necrosis. Gc concentration were measured using an EIA in double sandwich technique with purified Gc as standard. Mean levels of serum Gc were 6.0 +/- 7.4 mg/dl in fulminant hepatitis, 20.8 +/- 13.6 mg/dl in acute hepatitis and 39.4 +/- 13.8 mg/dl in normal subjects respectively. There was slight correlation between Gc and PTT (P less than 0.05). In the observation of sequential study in patients with fulminant hepatitis, serum Gc levels of live cases elevated but those of dead cases slightly elevated, thereafter decreased. These results suggested that serum Gc levels was the useful marker as a grade of liver cell damage and a parameter of regeneration in liver cells.

Acute Disease↗
PubMed1989

A new urinary mass screening system for neuroblastoma in infancy by use of monoclonal antibodies against VMA and HVA.

As an assay system for mass screening of infants with neuroblastoma, a new method of determining urinary vanillylmandelic acid (VMA) and hemovanillic acid (HVA) levels--an enzyme immunoassay (EIA) method--was developed. By using two different monoclonal antibodies, one against VMA and the other against HVA, this system can assay 400 urine samples for both catecholamine metabolites in five hours. By measuring both VMA and HVA levels in 275 urine samples (62 from patients with neuroblastoma, 13 from patients with control tumors, 200 from healthy infants as controls) by the EIA method, as well as by high performance liquid chromatography (HPLC), we examined whether the EIA system is as accurate as the HPLC. There were significant correlations between values obtained by the two systems, both for VMA and for HVA, suggesting that the EIA method would be a more reliable and convenient system for mass screening of infantile neuroblastoma as compared with conventional qualitative tests with inevitable high false-positive rate.

Antibodies, Monoclonal↗
PubMed1989

Molecular cloning and sequencing of a cDNA of rat dopa decarboxylase: partial amino acid homologies with other enzymes synthesizing catecholamines.

Dopa decarboxylase (DDC; aromatic-L-amino-acid decarboxylase; aromatic-L-amino-acid carboxylase, EC 4.1.1.28) was purified from rat liver and its partial sequence was determined. Synthetic oligonucleotides were used to construct and screen rat liver cDNA libraries, and three clones were isolated and sequenced. The 2 kilobases of DDC cDNA cloned consisted of a 5'-noncoding segment of 78 nucleotides, a coding region of 1440 nucleotides, and a 3'-noncoding region of 438 nucleotides. The encoded protein of 480 amino acid residues had a molecular weight of 54,000. A special feature of the primary structure of rat DDC was a repeating structure consisting of 29 amino acid residues. A sequence of 58 amino acid residues, including this repeating structure of rat DDC, was found to show homologies with those of rat tyrosine hydroxylase, human dopamine beta-hydroxylase, and bovine phenylethanolamine N-methyltransferase, other mammalian enzymes that synthesize catecholamines. These results indicate that catecholamine biosynthetic enzymes are structurally related and suggest that their homologous domains are important for catechol-protein interactions.

Amino Acid Sequence↗
PubMed1989

Mean cellular volume of urinary red blood cells in investigation of hematuria.

The mean cellular volume (MCV) of urinary red blood cells (RBCs) of pediatric patients with glomerular (group I; n = 52) and nonglomerular (group II; n = 21) hematuria was determined using an automated blood-cell analyzer. Group I patients had a significantly lower MCV than group II (61 +/- 8 vs. 88 +/- 15 microns 3, mean +/- SD; p less than 0.001). With a MCV of 75 microns 3 taken as the dividing line between glomerular and nonglomerular hematuria, correct assessment of the site of bleeding was made in 67 of the 73 (92%) patients studied. Determination of the MCV of urinary RBCs is a simple noninvasive test for localizing the site of hematuria in pediatric patients.

Adolescent↗
PubMed1989

Formation of hydroxynorspermidine from exogenously added 1,3-diaminopropan-2-ol in Vibrio species.

When Vibrio parahaemolyticus AQ 3627 was grown in the presence of 1,3-diaminopropan-2-ol (OH-Dap), a new compound accumulated in the cells. This was identified as hydroxynorspermidine (OH-Nspd), N-(3-aminopropyl)-1,3-diaminopropan-2-ol, by gas chromatography-mass spectrometry and thin-layer chromatography. It was also synthesized enzymatically from OH-Dap by a cell-free preparation from this strain. All other Vibrio strains examined also showed the ability to synthesize this compound, but none of the non-vibrio organisms did, indicating that OH-Dap is an in vivo substrate for the enzyme responsible for biosynthesis of norspermidine characteristically present in vibrios. These results suggest that the ability to synthesize OH-Nspd from OH-Dap present in the growth medium may be useful as an additional identifying factor for the genus Vibrio.

Biotransformation↗
PubMed1989

[VM-26 and VP-16 salvage therapy for refractory germinal testicular cancers].

Thirteen evaluable patients with germinal testicular cancers failing to be cured with first-line therapy (refractory) were treated by salvage chemotherapy. Ten patients received salvage chemotherapy with VM-26 (50 mg/m2, twice a week X 6 weeks) and cisplatin (CDDP, 20 mg/m2 for 5 consecutive days every 3 weeks for 3-4 times) (P-VM), 3 patients were also treated by radiation therapy, and 3 patients received VP-16 (100 mg/m2) and CDDP (20 mg/m2) (P-VP), all given daily for 5 consecutive days every 3-4 weeks for 4-5 courses. Of 13 evaluable patients, 6 (46%) had complete response (CR) (three cases were also treated with radiation therapy), 4 (31%) achieved partial response (PR), and 3 (23%) had no response. Limited to 7 patients treated with only P-VM therapy, there were 3 (43%) CR and 4 (57%) PR. Nine patients (69%) remained alive and were continuously disease free 18 to 84 months (median 48 months). Hematologic toxicity was severe, but with no death related to sepsis. Salvage chemotherapy with VM-26 or VP-16 and cisplatin offers potentially curative treatment to patients with refractory testicular cancer. The addition of radiation therapy to salvage chemotherapy was also effective.

Adult↗
PubMed1989

[Clinical evaluation of chemotherapy in urologic cancer].

In this paper, we will attempt to place the current status of the chemotherapy for testicular cancer, urothelial tumors and penile cancer in perspective. Perhaps the most exciting development in the past decade has been the demonstration of a significant number of complete responders (CR) to CDDP combined chemotherapy in patients with advanced testicular cancers. A CR rate of 60% was observed in the PVB therapy or VAB-6 therapy and no evidence of disease (NED) increased to 80% with salvage therapy. In our study of 43 evaluable patients, the CR rate was 60% and the NED rate was 81%. The activity of salvage chemotherapy with VP-16 for refractory testicular cancer has been well known with a CR rate of 19-38%. Among 13 evaluable patients with refractory cases, the CR rate was 46% with VP-16 or VM-26 salvage chemotherapy. The chemotherapeutic results of advanced urothelial cancers were also reviewed. In the present time, M-VAC therapy is thought to be the most effective regimen with a CR rate of 37%. In the chemotherapy of the primary lesion of penile cancer, bleomycin is most effective with a response rate of 60-70%. But for the primary lesion, there has been no effective chemotherapy.

Adult↗
PubMed1989

[A case of exohepatic pedunculated hepatocellular carcinoma suspected of adrenal tumor].

A case of exohepatic pedunculated hepatocellular carcinoma that was clinically diagnosed as nonfunctioning adrenal tumor is reported. A 66-year-old man was admitted to our hospital for further examination of unstable angina pectoris. Abdominal echogram and CT scan revealed a large tumor in the right retroperitoneal space. Selective right renal arteriography demonstrated that the tumor was fed by the capsular branch of right renal artery and the right adrenal artery. Adrenal function was normal. Preoperative diagnosis of right nonfunctioning adrenal tumor was made. On operation we found that the tumor was pedunculated from the liver and adhered massively to both right kidney and vena cava. The tumor and right kidney were removed. A histopathological examination demonstrated well differentiated hepatocellular carcinoma (Edmondson's grade II type).

Adrenal Gland Neoplasms↗
PubMed1989

[Evaluation of a modified latex agglutination inhibitory reaction method for the quantitation of urinary estriol in pregnant women].

This study evaluated a modified latex agglutination inhibitory reaction method for the quantitation of urinary estriol in women during normal and abnormal pregnancy. The urinary levels of estriol in pregnant women measured by this method correlated with urinary and serum levels measured by radioimmunoassay. The urinary level of estriol rose during pregnancy and reached a peak at the time of delivery, after which it returned to the control level in a few days. In contrast, the urinary level of estriol in women with abnormal pregnancy did not rise during pregnancy. This method is useful to detect impaired function of the placenta in pregnant women.

Estriol↗
PubMed1989

[A new role of uric acid as an antioxidant in human plasma].

Free radical attack upon uric acid (UA) nonenzymatically generates allantoin (ALT), and the presence of ALT in human plasma suggests free radical intervention within the body. To assess this possibility, we determined plasma ALT in patients with chronic renal failure (CRF) and some other diseases by high-performance liquid-chromatography (HPLC). Heparinized blood samples were obtained from 15 healthy controls, CRF patients under conservative management (n = 13) or hemodialysis (HD) treatment (n = 8) and patients with gout (n = 11) or rheumatoid arthritis (RA, n = 13). Although not seen in normal plasma samples, ALT was detected in 63% and 31% of patients receiving HD and conservative treatment, respectively. The plasma ALT level decreased after each HD session. ALT was also detected in 18% and 23% of the patients with gout and RA, respectively. ALT was found to be generated by ultraviolet radiation or by the addition of H2O2 to a normal pool-plasma. Addition of Fe(2+) and H2O2 increased the ALT level to about twice that of only H2O2. Addition of either catalase, desferal, EDTA, DMTU, DMSO or mannitol to the plasma decreased ALT generation. These findings suggest that ALT is generated from UA attacked by free radicals, especially by the hydroxyl radical, and that UA plays a role as an antioxidant in the plasma of patients with CRF and some other diseases.

Allantoin↗