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Biomedical subjects

M Kubín

Publications and source records attributed to M Kubín.

At least 73 records · Page 4Linked to original sources
PubMed1980

In vitro studies of cell-mediated immunity in rabbits sensitized with Mycobacterium kansasii and Mycobacterium tuberculosis.

Rabbits, sensitized with M. kansasii, responded by a profound inhibition of migration of macrophages elicited by both antigens: the migration index for homologous antigen was 0.51 in the direct test and 0.65 in the indirect test; for heterologous antigen the indexes were 0.53 and 0.67. However, significant differences in reactivity were found in rabbits sensitized with M. tuberculosis. In the homologous system, high reactivity was maintained and the migration index reached the value of 0.53 in the direct and 0.63 in the indirect test. On the other hand, the heterologous antigen M. kansasii influenced the migration in both direct and indirect assays significantly less, the migration indexes being 0.62 and 0.72. The differences were statistically significant at 1% and 5% levels.

Animals↗
PubMed1980

Mycobacterium kansasii infection in an endemic area of Czechoslovakia.

Over the period 1968-1979, Mycobacterium kansasii was isolated from a total of 297 persons residing in the district of Karviná, North-Moravian Region, Czechoslovakia. Repeated M. kansasii isolations were recorded in 153 patients with clinical symptomatology of chronic respiratory disorders, single isolations in another 144 persons (121 isolations from sputum and/or laryngeal swabs, 22 from urine, and one from the aspirate of a lymph node with tuberculoid granuloma). The incidence of infection with repeated M. kansasii isolation increased from 1.1 to 11.8 per 100 000 population. The ratio of these infections to the number of newly notified cases of pulmonary tuberculosis increased from 3.7 to 33.7%. The average age of patients was 46 years, 84% of all notified cases occurred by 54 years of age and morbidity was highest in the age group 45-54 years and reached 143/100 000 population. The proportion of male patients was 88% and 56% were heavy industry workers occupationally exposed to high dust concentrations.

Adult↗
PubMed1980

Cellular immunity in natural and experimental infections caused by Mycobacterium tuberculosis and Mycobacterium kansasii.

In rabbits sensitized with M. tuberculosis and M. kansasii the of macrophage migration inhibition from the spleen due to antigens of both types of mycobacteria was evaluated. In parallel experiments the release of MIF the indirect migration inhibition was evaluated, using antigens added to lymphocyte suspensions from popliteal nodes of sensitized animals. As an indicator system spleen fragments of intact rabbits were used. In patients excreting M. tuberculosis and M. kansasii (groups of 31 subjects) the release of MIF from lymphocytes in venous blood was investigated, using the same antigens; migration inhibition was also tested on spleen fragments of intact rabbits. Rabbits sensitized with M. kansasii responded by marked migration inhibition induced by both antigens in the direct as well as the indirect test. Marked differences were, however, manifested in animals sensitized with M. tuberculosis: in the homologous system a high reactivity was preserved, however, the heterologous antigen of M. kansasii influenced the migration in the direct and indirect test much less. These differences of reactivity were statistically significant at the 1% and 5% level of significance resp. Similar results as in model experiments were recorded also in patients naturally infected with M. tuberculosis and M. kansasii. In subjects with M. kansasii migration inhibition induced by both antigens was recorded and the differences were not statistically significant. In patients with M. tuberculosis the reactivity to the homologous antigen was more marked than to the heterologous antigen of M. kansasii and this difference was significant at the 1% level of significance.

Animals↗
PubMed1979

Lymph node activation by a factor released from lymphocytes of normal mice in reaction with Mycobacterium kansasii.

Substances which have the same properties as the previously described lymph node activating factor are released from the 4-h culture of 10 X 10(6) lymph node cells from normal non-sensitized mice and 2.5--5 X 10(6) Mycobacterium kansasii cells. They increase the number of lymphocytes with nucleoli synthesizing ribonucleic acid in the popliteal lymph nodes of intact mice and give the precipitation lines with the beta- to alpha-globulin electrophoretic mobility, which are specific for the lymph node activating factor, in reaction with serum against the supernatants from mixed lymphocyte cultures. The conclusion drawn from these observations is that the release of the factor or related substances is one of the more general manifestations of the early response of lymphocytes to foreign cells. The activating and in immunoelectrophoresis specifically reacting substance is not released from lymphocytes of mice from a specific pathogen-free colony. We assume that the release of the factor is not the primary reaction of the non-sensitized lymphocytes but the secondary reaction of lymphocytes presensitized with substances which share antigenic determinants with Mycobacteria.

Animals↗
PubMed1978

Treatment of solid Gardner lymphosarcoma with methotrexate sorbed on 2-hydroxyethylmethacrylate polymer in combination with leukovorin.

Implantation of 2-hydroxyethylmethacrylate microporous carrier with sorbed methotrexate (Hema-Hex-MTX) proved more toxic on C3H strain mice with a solid Gardner lymphosarcoma than on tumor-free mice of the same strain. If, during the first week of the experimental disease, the Hema-Hex-MTX implantation was followed by administration of leukovorin in course of next 24 hours the toxicity of MTX was abolished and the treated mice survived significantly longer than control animals.

Animals↗
PubMed1977

Effect of methotrexate sorbed on modified 2-hydroxyethylmethacrylate carriers in mice of C3H strain with a solid Gardner lymphosarcoma.

A aminohexylderivative of 2-hydroxyethylmethacrylpolymer has been prepared in the form of a microporous sponge, and Methotrexate (MTX) has been bound to this carrier by chemical sorption. Implantation of the carrier with sorbed MTX resulted in a decrease of tumor weight, while an implantation of the carrier alone or an i.p. injection of an identical dose of MTX had no effect on tumor growth. Tumor regression was found to be greater than after a repeated injection of MTX in a fourfold dose directly into the tumor.

Acrylic Resins↗
PubMed1977

[The stratified tuberculoma caused by Mycobacterium kansasii with cavitation (author's transl)].

Report on a 39 years old female textile worker with an acute cavity. M. kansasii was repeatedly isolated by cultivation from the sputum. The patient became negative by treatment with INH, SM, PAS, Ethionamide, but the cavity persisted. Therefore seven months after the onset of the disease the left upper lobe was resected. A colliquative Stratified tuberculoma and some lesions of caseated bronchitis and peribronchitis were found in the neigh bourhood. Clusters and microcolonies of M. kansasii could be observed as well in the wall of the cavity as in the centre of caseated bronchial lesions. The mycobacteria had a characteristic shabe of long segmentated rods. All about the microcolonies situated in the lumina of small bronchi and bronchioli caseated lesions were present, surrounded by specific granular tissue consisting of epithelial cells, some Langhans giant cells, lymphocytes and fibrocytes. The alterations caused by M. kansasii did not differ from those lesions caused by M. tuberculosis (human.) formerly described as stratified tuberculoma and caseated bronchial lesions with mycobacteria centrally located.

Adult↗
PubMed1975

Endocellular parasitism of Mycobacterium avium in rabbit liver. A morphological study.

Yersin's type of experimental tuberculosis was induced in rabbits by intravenous administration of Mycobacterium avium and was studied in situ in the liver. Mycobacteria were only found in Kupffer-cell phagosomes and, at the later stages of the disease, in the phagosomes of the multinuclear giant cells. Ultrastructural changes in Kupffer's cells amounted to an increase in cytoplasm volume and corresponding increase in the number of ribosomes, endoplasmic reticulum and mitochondria. As compared with control animals, Kupffer's cells were more frequent in the experimental rabbits, clustering around sinusoid capillaries. Characteristic changes in mycobacterium ultrastructure were cytoplasmic vacuolisation in some individuals and an almost regular presence of high-density polymorphous structures. The microbial cell walls remained intact.

Animals↗