Search PubMed⌕ Search

Biomedical subjects

M Krzyzanowski

Publications and source records attributed to M Krzyzanowski.

80 records · Page 5Linked to original sources
PubMed1976

A modified method for isolating alpha-amylase from rabbit skeletal muscle.

A modified method for purifying alpha-amylase from rabbit skeletal muscle is described, which in comparison with the previously published method gives better results of isolation and is economical, less time-consuming, and require less material. The former method gave 1,340-fold purification of the enzyme with a yield of 0-6%, and the new method 2,014-fold purification and 7-6% yield. Electrophoresis in polyacrylamide gel showed absence of a strictly defined zone of enzyme activity and presence of inactive proteins.

Amylases↗
PubMedDate not supplied

Relation of peak expiratory flow rates and symptoms to ambient ozone.

The temporal association between peak expiratory flow rates (PEFRs) and ambient ozone (O3) was studied in a group of 287 children and 523 nonsmoking adults in Tucson. In children, noon PEFRs were decreased on days when there was a higher O3 concentration; children with physician-confirmed asthma experienced the greatest decrease in noon PEFR. Evening PEFR levels were also significantly related to O3 in children, especially asthmatics. Among adults, evening PEFRs were decreased in asthmatics who spent more time outdoors on days when O3 levels were higher. After we adjusted for covariates, significant effects of interactions of 8-h O3 levels with particulate matter (PM10) and temperature on daily PEFR were found. There was some overnight effect of 8-h O3 on morning PEFRs. In general, the respiratory response to low-level ambient O3 is acute, occurs more in asthmatics, and increases as temperature and PM10 increase.

Adolescent↗
PubMedDate not supplied

Multipollutant exposures and health responses to particulate matter.

Epidemiological methods provide opportunities to study interactions of pollutants in complex environments. During the study of health and the environment and the evaluation of particulate matter in Tucson, we found that type, location, and temporality of particulate matter exposures were critical with respect to the various interactions that related to health effects. Indoor particulate matter interacted with other components of particulate matter found in tobacco smoke, as evidenced by lung function. The interaction of environmental tobacco smoke with indoor formaldehyde caused various symptoms. Other interactions occurred between indoor and outdoor forms of particulate matter, which caused symptoms in some of the subjects.

Air Pollutants↗
PubMedDate not supplied

[Monitoring of the treatment of endogenous depression with imipramine and amitriptyline (preliminary report)].

Monitored treatment of a depressed phase of unipolar affective disorder was conducted in 11 female patients receiving imipramine and in 12 females taking amitriptyline. Patients were randomly assigned to one of the drug and in 6 patients the drugs were switched because of the lack of response to the first used compound. In the imipramine treated group a satisfactory response after 4 weeks of management (less than 6 points on Hamilton's depression scale) was observed in 6 patients and in amitriptyline treated group in 5 patients. Patients displaying a satisfactory response to amitryptyline had significantly higher--as compared to remaining patients in the group--plasma levels of the drug after two and four weeks of treatment. Such an association was not observed in patients treated wtih imipramine. Severity of depression and motor retardation before the treatment was similar both in patients with satisfactory and with poor response to imipramine as well as to amitriptyline. However the intensity of anxiety symptoms was higher in patients exhibiting poor response to treatment with amitriptyline and imipramine as well.

Adult↗
PubMedDate not supplied

Air pollution and daily mortality in Cracow.

Several studies indicated that short-term increases in concentration of pollution in the atmosphere of a town may result in increased mortality in the days following the episode. To evaluate this relationship we analyzed data on daily mortality in Cracow, Poland, in the period 1977-1989 and data on daily concentrations of suspended particulates (PM 20) and sulfur dioxide (SO2) in the town area. This analysis was restricted to winter months. In 1167 winter days, an average of 18.4 deaths of Cracow residents occurred daily. PM 20 concentration exceeded 300 micrograms/m3 in 21%, and SO2 exceeded 200 micrograms/m3 in 19% of those days. The correlation coefficient of the daily number of deaths with the air pollution in the 24 hours preceding the day of death was 0.093 (p < 0.01) for PM 20, and was 0.141 (p < 0.001) for SO2. Similar correlations were found for air pollution levels in the four days preceding death, and the correlation decreased for earlier days. The effects were more pronounced in people over 65 years of age than in those younger, especially in males. After adjustment for SO2 levels, PM 20 had no additional effect on mortality. It was estimated that with an increase of SO2 concentration of 100 micrograms/m3, the daily number of deaths due to respiratory system diseases increased by 19% and deaths due to circulatory system diseases by 10%.

Age Factors↗