Search PubMed⌕ Search

Biomedical subjects

M Krutsay

Publications and source records attributed to M Krutsay.

At least 73 records · Page 4Linked to original sources
PubMed1981

[Decalcification of histological specimens].

Authors studied the effect of various decalcifying solutions on the staining of cell nuclei. It remained normal within the period necessary for decalcification only when n-hydrochloric acid containing 2 per cent of ferric chloride or solution of EDTA III were used. Decalcification of solid bone specimens in hydrochloride acid-ferric chloride solution at room temperature can be completed in 6-7 days. The staining of cell nuclei remains normal by the 12th day. A satisfactory effect--decalcification in 14 days without any damage to the cell nuclei staining--can be achieved by a 27 per cent, pH 7.4 solution of EDTA III at a temperature 60 degrees C.

Decalcification Technique↗
PubMed1980

[Detection of acid mucopolysaccharides by hematoxylin].

Author studied the effect of the sequence of the dissolution of components of alumhaematoxylin solution with potassium iodate in it and the effect of the quantity of components on the staining capacity of the dye. Potassium iodate should be added after haematoxylin and alum are dissolved, otherwise the staining of the cartilage and mucus will be less successful. The best staining effect of tissue specimens containing acid mucopolysaccharides and cell nuclei as well can be achieved by the use of haematoxylin solution containing 0,5 per cent of alum. Sections prepared by the freezing technique should be stained by haematoxylin containing 5 per cent of alum.

Cell Nucleus↗
PubMed1980

[Fixation of tissue specimens].

Authors studied the efficacy of various fixatives used in pathohistology. For general purposes and for impregnation techniques sodium-acetate-formalin, for the demonstration of cytoplasmic granula of endocrine cells and for staining of connective tissue elements Ort's solution and formalin with chrome-alum should be used. Demonstration of glycogen seems to be optimal after the use of the mixture of picric acid- acetic acid and alcohol as a fixative.

Fixatives↗
PubMed1979

Spread and detection of psoroptic scabies of cattle in the United States.

Psoroptic scabies of cattle causes substantial economic losses. An eradication program begun in 1905 virtually eradicated the disease by 1950. Complacency was followed by a resurgence of the disease, particularly in the 1970s, when more than 1,000 outbreaks were reported. Program effectiveness was reduced when the disease was often not detected or reported for several months after herds became infested. Further spread was enhanced by infested cattle moving undetected through market channels.

Animals↗
PubMed1979

[The use of ethyl acetate for the embedding of tissue specimen].

Author for the prevention of detrimental health effects of benzene-homologs instead of xylene recommends the use of aethyl-acetate for the embedding process. It dissolves the warm paraffin, easily evaporates, mingles with acetone and isopropyl-alcohol and does not harden the tissue specimens.

Acetates↗
PubMed1979

[Modified trichrome staining].

Author in the process of the trichrom staining method instead of Orange-G, acid fuchsine and aniline-blue applied other dyes. For the staining of thin sections ponceau-S was found more suitable than acid-fuchsine. For the staining of thick sections the cerommends acid-fuchsine, light-green and fast-green FCF.

Rosaniline Dyes↗
PubMed1978

[Granular cell myoblastoma].

Authors report a case of multiplex myoblastoma of the skin observed at a male patient aged 34. Epidermies covering the tumour histologically did not show hyperplasia considered to be characteristic. 8 years after the removal of the tumour the patient is well.

Adult↗
PubMed1978

[Eosinophilic granuloma of the stomach].

Two cases of the eosinophil granuloma of the stomach are reported. The male patients (52 and 48 years old) had had gastric disorders. By the X-ray examination the suspition of a tumour had been raised. At both patients resection of the stomach by Billroth II was performed. The tumours histologically were eosinophil granulomas.

Eosinophilic Granuloma↗