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Biomedical subjects

M Kono

Publications and source records attributed to M Kono.

At least 325 records · Page 18Linked to original sources
PubMed1983

Isolation and pathogenicity of provisional serovar 1621-54 of "Shigella" from imported cynomolgus monkeys.

The provisional serovar 1621-54 of "Shigella" was isolated from 36 (13.2%) of 273 cynomolgus monkeys newly imported into Tsukuba Primate Center for Medical Science (TPC) from Indonesia during the period from 1980 through 1981. The biochemical properties of the isolates were in good agreement with those of previously described provisional "Shigella" serovar 1621-54. Clinically, 13 (36.1%) of 36 monkeys being positive for the organisms excreted diarrheal stools on arrival at TPC. In addition, the organisms were isolated also from three other monkeys contracting dysentery during the quarantine period at TPC. One of them died 2 days after the onset of dysentery. Histopathological findings of this case were essentially the same as those of Shigella-infected monkeys. Pathogenicity of the isolates was examined in three experimental infection models; (1) the infection model using cultured HeLa-S3 cells, (2) the keratoconjunctival infection model of the guinea pig, and (3) the intestinal infection model of the cynomolgus monkey. All the strains tested showed positive results in these experimental models, causing intracellular penetration and multiplication in HeLa-S3 cell cultures, keratoconjunctivitis in guinea pigs, and dysentery in cynomolgus monkeys. These results indicate that provisional "Shigella" serovar 1621-54 is a causative agent of dysentery in man as well as in monkeys.

Animals↗
PubMed1982

[Antibacterial activity of netilmicin on cefazolin resistant Staphylococcus aureus].

Many strains of cefazolin (CEZ)-resistant Staphylococcus aureus of recent clinical isolates were also resistant to gentamicin (GM). In addition, the antibacterial activity of NTL was found to be stronger than that of GM and stronger than that of CEZ for those resistant strains and especially for those moderately resistant to GM.

Cefazolin↗
PubMed1981

An enzyme immunoassay for serum tobramycin.

A heterogeneous enzyme immunoassay for serum tobramycin was developed using tobramycin-beta-D-galactosidase conjugate as the labelled antigen and 4-methylumbelliferyl-beta-D-galactoside as the fluorescence substrate. B/F separation was carried out using an immobilized second antibody, Immunobead. The minimal detectable level was 10 pg/tube or 0.2 microgram/ml of serum. Assay recovery was excellent and the intra-assay and inter-assay variances were 4.41% and 12.6%, respectively. The values from the enzyme immunoassay agreed well with those from radioimmunoassay and bioassay. This assay system is highly sensitive and can be easily applied to other drugs and hormones.

Anti-Bacterial Agents↗
PubMed1981

An enzyme immunoassay for plasma betamethasone.

A sensitive enzyme immunoassay for plasma betamethasone was developed using betamethasone-3-(O-carboxymethyl)oxime-beta-D-galactosidase conjugate as a labelled antigen and 4-methylumbelliferyl-beta-D-galactoside as a fluorescence substrate. The performances of the enzyme immunoassay were compared with that of a radioimmunoassay using 3H-betamethasone and the same antiserum. The minimal detectable level for the enzyme immunoassay was 0.15 pg/tube or 0.15 ng/ml of plasma, which was remarkably more sensitive than the radioimmunoassay level of 10 pg/tube or 2 ng/ml of plasma. The specificity was sufficient, in particular, the cross reactivity of cortisol as 0.008%. However, the precision of the enzyme immunoassay was inferior to that of the radioimmunoassay.

Betamethasone↗
PubMed1981

Effects of carotid body chemoreceptor stimulation on respiration and phrenic nerve activity in intact and vagotomized rabbits.

The effects of carotid body chemoreceptor stimulation by NaCN on respiration and phrenic nerve activity were studied in intact and vagotomized rabbits. In intact animals, an intracarotid injection of 30 micrograms of NaCN resulted in an elevation of phrenic nerve activity and a rapid onset of respiratory excitation associated with an increase in respiratory rate and the response was markedly potentiated after vagotomy. The change in respiratory rate was primarily due to a decrease in expiration time in intact animals, whereas it resulted from a pronounced decrease in inspiration time in vagotomized animals. The results suggest a suppressive effect of the vagus nerve on carotid body chemoreceptor reflex and a possible interaction of the latter input with the pneumotaxic center. An induction of a continuous increase in phrenic nerve activity accompanied by apneustic respiration by intracarotid dopamine was another evidence to support the assumption.

Animals↗
PubMed1980

A sensitive enzyme immunoassay for plasma cortisol.

A sensitive enzyme immunoassay for plasma cortisol has been developed, using cortisol-beta-D-galactosidase conjugate as labelled antigen and 4-methylumbelliferyl-beta-D-galactoside as fluorescence substrate. Sensitivity and precision were compared with those of radioimmunoassay with 3H-labelled cortisol and the same antiserum. The minimal detectable level for the enzyme immunoassay was 10 pg/tube or 1 microgram/dl of plasma, which makes it slightly more sensitive than the radioimmunoassay and 10 times more sensitive than previously reported enzyme immunoassays. However, the precision of the enzyme immunoassay was inferior to that of the radioimmunoassay.

Galactosides↗
PubMed1980

Mycoplasma pulmonis arthritis in congenitally athymic (nude) mice. Histopathological features.

Histopathological examinations were performed on arthritis joints and other organs of strain BALB/cA nu/nu and nu/+ mice intravenously injected with Mycoplasma pulmonis strain m53. In both groups of mice suffering from polyarthritis, acute inflammatory lesions with infiltration of polymorphonuclear leukocytes in the synovia and periarticular tissues were observed one to two weeks after injection. In nu/nu mice, the acute inflammation appeared repeatedly up to 20 weeks after inoculation, when the experiment was terminated, and furthermore, extensive synovial and periarticular necrosis were characteristically present after the 4th week. Only a small number of lymphocytes and plasma cells were in the lesions. In nu/+ mice, after the early acute inflammation of arthritis, relapses of the infiltration of polymorphonuclear leukocytes were also observed in some mice in and after the 10th week. In addition, infiltration of lymphocytes and plasma cells were substantial after the 15th week. Focal necrosis was sometimes found in the liver of nu/nu mice. Perivascular infiltration of small lymphocytes and plasma cells was found in the lungs, liver and kidney of nu/+ mice in and after the 15th week. Repair mechanisms of injured articular tissues in nu/nu mice were histopathologically poor, while those in nu/+ mice seemed to be progressive and quite similar to those reported by many investigators for mice with the thymus intact. The histopathological differences are discussed in respect to the thymus-dependent immune responses.

Animals↗