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Biomedical subjects

M Kondo

Publications and source records attributed to M Kondo.

At least 1,171 records · Page 65Linked to original sources
PubMed1985

[A study on transference of cefminox into intrapelvic tissues].

Intravenous administrations of cefminox (CMNX, MT-141) 1 g as both single shot injection and drip infusion were absorbed rapidly. CMNX showed good transference into intrapelvic organs such as uterus, oviduct and ovary, with the tissue/serum ratios of 37.6% for myometrium, 35.1% for endometrium, 41.4% for cervix uteri, 49.5% for portio vaginalis, 54.3% for ovary and 59.7% for oviduct. From these results, usefulness of CMNX in the field of obstetrics and gynecology was confirmed.

Cephamycins↗
PubMed1985

Radiochemical microassay of delta-aminolevulinic acid dehydratase activity in whole blood and bone marrow.

We describe an assay for delta-aminolevulinic acid dehydratase (EC 4.2.1.24) activity. Radioactive [14C]porphobilinogen, formed by action of this enzyme on [14C]delta-aminolevulinic acid, is purified by passage through an ion-exchange chromatographic column before measurement with a liquid scintillation counter. The radioactive substance in the final solution was identified as solely [14C]porphobilinogen by paper-chromatographic analysis. The present assay procedure requires only a 0.1-microL sample of blood and is about 100-fold more sensitive than the conventional colorimetric methods involving Ehrlich's reagent. Using this method, we found that activity of this enzyme in the bone marrow of rats decreases abruptly and sharply two weeks after birth.

Animals↗
PubMed1985

[Digital perfusion images for evaluating patients with mitral valve replacement].

To determine whether the distribution of pulmonary perfusion can be applied as a noninvasive means of evaluating patients with mitral valve replacement (MVR), computerized Tc-99m MAA pulmonary perfusion images (digital perfusion images: DPI) were obtained for 32 patients in the preoperative, early postoperative and late postoperative (18 months mean) periods. DPI consisted of isocount areas, and a 100-70% area was defined as a hyperperfusion area. The distribution of pulmonary perfusion was evaluated using patterns of hyperperfusion area in anterior DPI. In 32 patients above-mentioned had the hyperperfusion areas in the upper lung fields preoperatively, the perfusion of the lung base was investigated. In 21 patients hyperperfusion area appeared in the lung base in the late follow-up period (group A), and all patients improved clinically. In 11 patients, the DPI improved to nearly normal patterns. In five patients, the DPI improved after one year postoperatively. In 11 patients, hyperperfusion areas did not appear in the lung base in the late follow-up period (group B). Six of the 11 patients did not improve clinically. There were no significant hemodynamic differences between groups A and B except for slight differences in the postoperative pulmonary vascular resistance. These results suggested significant correlations between the clinical improvement and the normalization of the DPI. The use of DPI patterns may facilitate quantitative and objective estimations of postoperative states. Since DPI are easy to perform noninvasively, DPI may comprise a useful graphic diagnostic method for evaluating patients with MVR.

Adolescent↗
PubMed1984

Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II.

The nucleotide sequence of the cloned DNA, 1,478 bp in length coding for glutathione synthetase (GSH-II) of E. coli B has been determined. Amino acid and nucleotide sequence analyses have assigned the open reading frame for GSH-II, starting with the ATG near its 5' terminus. The molecular weight calculated from the predicted amino acid sequence is 35,559 daltons, being in good agreement with that of a GSH-II subunit estimated by the SDS-PAGE method. Several signal sequences conserved in the promoter regions of E. coli were found in the non-coding regions of the gsh-II gene. They include the Shine-Dalgarno sequence, the Pribnow box and the sequence conserved in the "-35 region" with a preferable spacing from each other for an efficient transcription. Downstream from the termination codon, the inverted repeat sequences were present, followed by 6 successive T's. These structural features found in the non-coding regions have suggested to be involved in regulatory functions for the gsh-II gene expression.

Amino Acid Sequence↗
PubMed1984

Endotoxin-induced disseminated intravascular coagulation in vitamin E deficient rats.

The effect of vitamin E on endotoxin-induced experimental disseminated intravascular coagulation (DIC) was studied in rats deficient in and supplemented with vitamin E. Experimental DIC was induced by a 4-hr sustained infusion of endotoxin at a dose of 13.3 mg/kg. After the infusion, fibrinogen and fibrin degradation products were increased, platelet count and fibrinogen level were decreased, and prothrombin time and partial thromboplastin time were prolonged. In addition, the number of renal glomeruli with fibrin thrombi was increased. These changes were significantly greater in vitamin E deficient rats when compared to those changes found in rats supplemented with vitamin E. These results indicate that vitamin E plays a protective role in endotoxin-induced DIC.

Animals↗
PubMed1984

Patterns of relapse of squamous cell carcinoma of the maxillary sinus.

Ninety-five M0 patients with squamous cell carcinoma of the maxillary sinus were treated mainly with combined surgery, radiation and chemotherapy. They were staged surgically according to the American Joint Committee TNM classification: 3 were T2N0, 55 T3N0, 27 T4N0 and 10 T3-4N1-3 patients. Relapse developed in 66 patients (69%): one (33%), 35 (64%), 22 (81%) and 8 (80%), respectively. All patients experienced relapse within 2 years after diagnosis. Local recurrence developed during the course of disease in 52 (79%) of the 66 patients who relapsed. Three patients with cervical relapse and one with local recurrence were successfully salvaged with rescue surgery. Local control achieved in 39 (41%) of the 95 patients: 2 (67%), 28 (51%), 7 (26%) and 3 (30%) in T2N0, T3N0, T4N0 and T3-4N1-3 patients, respectively. Autopsy findings of 17 patients are presented. An impact of computed tomography on staging of maxillary sinus tumors is discussed.

Adult↗
PubMed1984

Relation of clinical activity of rheumatoid arthritis to immune complexes, complement components and anti-immunoglobulins.

Circulating immune complexes by fluid phase Clq binding assay, complement components and anti-immunoglobulin levels were studied in sera of 35 patients with rheumatoid arthritis (RA). In 23 of the 35 sera (65.7%), circulating immune complexes were positive, and the mean +/- SD of Clq binding activity (ClqBA), 44.5 +/- 19.4%, was significantly high compared to that of healthy persons, 17.4 +/- 8.2%. Antigenic determination of complement components revealed that Clq, C3, C5, C9, factor B and Cl esterase inhibitor (ClINH) were significantly high in sera of RA, but C4 and properdin were not. The disease activity correlated with ClqBA, IgG- and IgM-anti-immunoglobulins, C9 and serum IgG. On the other hand, ClqBA correlated with both IgG- and IgM-anti-immunoglobulin levels but not with complement components.

Adolescent↗
PubMed1984

An autopsy case of acute porphyria with a decrease of both uroporphyrinogen I synthetase and ferrochelatase activities.

An autopsy case of a 37-year-old woman with acute porphyria is reported. The patient began to complain of severe menstrual pains, and later developed serious peripheral neuropathy and various autonomic nervous symptoms. The autopsy revealed a marked loss and degeneration of axons and myelin sheaths in the peripheral nervous system (PNS), and prominent central chromatolysis of the spinal anterior horn cells. The predominant process of the peripheral neuropathy appeared to be axonal degeneration. Biochemical analysis showed a marked increase of delta-aminolevulinic acid (ALA), porphobilinogen, uroporphyrin, and coproporphyrin in the urine, and an increase of coproporphyrin and protoporphyrin in the stools and blood. In the analysis of the enzymatic activities of the liver and bone narrow, the activity of ALA synthetase (ALA-S) was markedly increased, and the activities of both uroporphyrinogen I synthetase (URO-S) and ferrochelatase were decreased. It was characteristic in this case that the enzymatic abnormalities found in both acute intermittent porphyria (AIP) and variegate porphyria (VP) coexisted. Biochemical analysis of the sciatic nerve showed an increase of ALA-S activity and a decrease of both URO-S and ALA dehydrase activities. This was the first report that indicated the presence of abnormal activities of the heme biosynthetic enzymes in the peripheral nerves of porphyric patients. The possibility was discussed that these enzymatic abnormalities of the heme biosynthesis in the peripheral nerve itself might be strongly related to the pathogenesis of the porphyric neuropathy.

Adult↗
PubMed1984

Evaluation of tricuspid regurgitation by blood flow pattern in the hepatic vein using pulsed Doppler technique.

Evaluation of tricuspid regurgitation (TR) was attempted by observing the blood flow pattern in the hepatic vein (HV), inferior vena cava dimension (IVCD), and hepatic vein dimension (HVD), with the use of a combined system of pulsed Doppler technique and two-dimensional echocardiography in 60 patients with valvular heart disease. For comparison, all patients underwent right ventriculography, by which TR was classified as mild, moderate, or severe. IVCD and HVD of the group with severe TR were significantly larger than those of the other groups (p less than 0.005). Several types of abnormal blood flow patterns in the HV were demonstrated in patients with atrial fibrillation. By these flow patterns, the severe or moderate TR groups could be differentiated from the mild TR group, because 21 of the 26 patients (81%) in the former group showed reverse hepatic flow during systole. Also, the Doppler shifts from baseline in the sound spectrogram correlated well with right atrial pressure (RAP) and right ventricular end-diastolic pressure (RVEDP) (r = -0.72, -0.64, p less than 0.001, respectively). This method is useful for rapidly evaluating considerable TR and for estimating RAP and RVEDP noninvasively.

Adult↗
PubMed1984

Reduced norepinephrine turnover in mice with monosodium glutamate-induced obesity.

The disappearance of norepinephrine from the heart, interscapular brown adipose tissue (BAT), and pancreas has been examined in mice with monosodium glutamate (MSG)-induced obesity and in untreated controls. MSG-treated mice became obese in the absence of increased food intake and their core temperature was significantly lower compared to control mice. The rate of norepinephrine turnover following blockade of norepinephrine synthesis with alpha-methyl-para-tyrosine was significantly slower in heart and interscapular BAT of these mice than in untreated controls, but MSG had no effect on the pancreas. It is suggested that reduced norepinephrine turnover may be a major factor in the development of obesity after neonatal administration of MSG.

Adipose Tissue, Brown↗