Search PubMed⌕ Search

Biomedical subjects

M Kondo

Publications and source records attributed to M Kondo.

At least 613 records · Page 34Linked to original sources
PubMed1995

The selective effects of dopamine and dobutamine on liver circulation in the dog.

The selective effects od dopamine and dobutamine in various doses on liver circulation were studied in 12 mongrel dogs. Dopamine increased portal flow but decreased hepatic arterial flow markedly as infusion rate of dopamine increased. Dopamine 3 micrograms/kg/min infusion rate produced vasodilation in mesenteric vascular bed and the portal flow ratio to cardiac output was significantly increased. Dobutamine increased both portal and hepatic arterial flows at the 5 and 10 micrograms/kg/min dobutamine infusion rates, and decreased hepatic arterial flow at the 15 micrograms/kg/min dobutamine infusion rate. Both dopamine and dobutamine increased total liver flows, however, total liver flow ratio to cardiac output was not increased. Pressure gradient of portal system was not changed during dopamine and dobutamine infusion, since both portal venous pressure and hepatic venous pressure were avariant from control values. These findings suggest that congestive hyperemia was not occurred in intrahepatic portal vascular system when portal flows were increased during dopamine and dobutamine infusion. The results of this study demonstrate that both dopamine and dobutamine did not produce selective increases in total liver blood flow. In addition, both agents should be safe to use to the normal liver patient; total liver blood flow did not decrease and intrahepatic congestive hyperemia was not occurred when portal flow was increased.

Animals↗
PubMed1995

Effect of food on liver circulation in conscious dog.

Hepatic hemodynamics and the liver oxygen supply-uptake relationship, in response to eating, were investigated in a chronically catheterized conscious dog method. Portal venous pressure was significantly increased after eating, however was within the normal range reported previously. Hepatic venous pressure correlated well with portal venous pressure throughout the experiment, therefore, the pressure gradient of the portal system was unchanged. Hepatic venous oxygen content, correlated well with liver oxygen extraction, was unchanged after eating. Therefore, it is possible to assume that liver oxygen supply-uptake relationship is well maintained during digestion of food.

Animals↗
PubMed1995

Eight cases of gastric tumors with calcification.

Gastric tumors with calcification have been considered to be relatively rare. We treated 8 cases of tumors involving calcification, 4 cases of gastric carcinomas and 4 other cases of submucosal tumors with ulceration. These cases are reported along with a review of the cases reported in Japan.

Aged↗
PubMed1995

Acromegalic gigantism with low serum level of growth hormone and elevated serum insulin-like growth factor-I.

In a case of acromegalic gigantism with hyperprolactinemia is reported, the basal serum growth hormone (GH) levels ranged from 1.2 to 1.9 ng/ml. Serum GH response to either insulin-induced hypoglycemia or GH-releasing hormone was blunted. Frequent blood sampling showed non-pulsatile GH secretion. Serum prolactin and insulin-like growth factor-I (IGF-I) levels were elevated. After unsuccessful surgery, bromocriptine treatment normalized serum prolactin without affecting serum GH and IGF-I levels. Combined administration of octreotide with bromocriptine reduced serum GH and IGF-I levels. In this case, non-pulsatile GH secretion and enhanced tissue sensitivity to GH may induce hypersecretion of IGF-I and cause clinical acromegalic gigantism.

Acromegaly↗
PubMed1995

Simultaneous intraperitoneal administration of OK-432 and serum enhances superoxide generation from migrated polymorphonuclear leukocytes, with special emphasis on the role of complements.

Superoxide and its derived active oxygen species are responsible for the polymorphonuclear leukocyte (PMN)-mediated tumoricidal activity which is typically shown in the intraperitoneal administration of OK-432, a biological response modifier, for cancer ascites. We examined the effects of intraperitoneal administration of OK-432 with or without syngeneic serum on superoxide generation from PMNs which migrated in the peritoneal cavity using the new method of Cypridina luciferin analog-dependent chemiluminescence for the detection of superoxide. PMNs harvested from rat peritoneal cavity 6 h after the intraperitoneal administration of OK-432 (0.25KE/kg, or 2.5KE/kg) generated larger amounts of superoxide than those harvested after intraperitoneal injection of oyster glycogen (75mg/kg) when stimulated by opsonized zymosan or phorbol myristate acetate. Simultaneous intraperitoneal administration of OK-432 and syngeneic serum induced a greater increase in PMN superoxide generation than OK-432 alone, which was reversed by a complement activation inhibitor (MX-1). Simultaneous injection of OK-432 and heat-inactivated syngeneic serum did not exhibit a significant increase in PMN superoxide generation as compared with OK-432 alone. These results provide pharmacological evidence to the satisfactory therapeutic effects of the intraperitoneal administration of OK-432 with or without serum for patients with cancer ascites, and indicate that complements, in particular C5a, are involved in this enhanced PMN-derived superoxide generation induced by the simultaneous injection of OK-432 and serum.

Animals↗
PubMed1995

Assessment of left ventricular myocardial perfusion and diastolic function during acetylcholine-induced diffuse coronary vasoconstriction by Doppler echocardiography and thallium-201 scintigraphy.

BACKGROUND: We investigated whether diffuse coronary vasoconstriction induced by acetylcholine caused myocardial ischemia. METHODS: We studied 30 patients (40 coronary arteries) with spontaneous chest pain or equivocal studies on treadmill exercise testing and no significant coronary stenosis or previous myocardial infarction. During the acetylcholine provocation test, Doppler echocardiography was performed, and thallium-201 was injected intravenously for scintigraphy. We used Doppler echocardiography to measure the ratio of early-to-late peak mitral flow (E:A ratio). RESULTS: When acetylcholine was injected, the coronary arteries showed three different responses. Diffuse coronary vasoconstriction without chest pain or ischemic changes on the ECG was induced in 18 (45%) arteries and the E:A ratio decreased from 0.83 +/- 0.13 to 0.77 +/- 0.13 (P = 0.031). In 17 vessels (control arteries), the E:A ratio did not change significantly (from 0.88 +/- 0.15 to 0.88 +/- 0.18; P = 0.95). In five arteries, focal spasm was induced and the E:A ratio decreased from 0.83 +/- 0.18 to 0.66 +/- 0.13 (P = 0.043). Transient defects on thallium-201 scintigraphy were observed in the territory of eight (80%) arteries with diffuse vasoconstriction and in one (20%) control artery (P = 0.047). CONCLUSIONS: Diffuse coronary vasoconstriction induced by intracoronary acetylcholine can decrease the regional myocardial blood flow (as shown by thallium-201 scintigraphy) and can cause global left ventricular diastolic dysfunction (as shown by the results of Doppler echocardiography).

Acetylcholine↗
PubMed1995

[Clinical significance of IgG rheumatoid factor in patients with rheumatoid arthritis].

We studied 135 patients with rheumatoid arthritis (RA) from February 1991 through June 1992 (mean period: 9 months) to measure serum IgG rheumatoid factor (IgG-RF) by the method of enzyme linked immunosorbent assay. In order to evaluate clinical significance of serum IgG-RF we compared the level of IgG-RF with some of the clinical and laboratory markers including disease activity index of RA and titers of IgM rheumatoid factor (IgM-RF) and IgA rheumatoid factor (IgA-RF). Positive IgG-RF (more than 2.0 on IgG-RF index) was observed only in 22.2% (30 patients) with the examination of the first collected serum samples but was increased to 41.5% (56 patients) by the case of total test samples. The number of patients with positive IgM-RF or positive IgA-RF test was significantly smaller in seronegative patient group than in seropositive patient group, whereas IgG-RF test showed no significant differences between these two groups. These indicate usefulness of consecutive test of IgG-RF for diagnosis of RA especially in seronegative patients. The mean of total score indicating radiographic bone destruction by Sharp's modified method was significantly higher in positive patients than in negative patients of IgG-RF. Multivariate analysis showed positive correlations between serum IgG-RF levels and erythrocyte sedimentation rates as well as between serum IgG-RF levels and Lansbury's indexes. These results suggest that the test of serum IgG-RF level is beneficial for daily management of patients with RA.

Arthritis, Rheumatoid↗
PubMed1995

[The study of chemotherapy via the reservoir to colo-rectal cancer patients with liver metastases].

From January 1986 to December 1994, we administered intra-arterial chemotherapy via the reservoir to 26 colo-rectal cancer patients with liver metastases. The protocol of this chemotherapy was administration of ADM 30 mg/body/4 wks, MMC 10 mg/body/2 wks and 5-FU 500 mg/body/2 wks. Responses to this chemotherapy were PR in 8 cases, NC 3 cases, PD 14 cases and unknown 1 case. The rate of response was 32.0%. Side effects were shown in 12 cases (46.2%), and trouble with the reservoir in 5 cases (20.8%). Survival times of patients were from 5 months to 42 months (average 13.6). We think that this intra-arterial chemotherapy via the reservoir was effective for patients with liver metastases from colo-rectal cancer.

Adult↗
PubMed1995

[A prospective randomized clinical trial comparing intra-arterial chemotherapy alone and when combined with hyperthermia for metastatic liver cancer].

To evaluate the efficacy of intra-arterial chemotherapy combined with hyperthermia for metastatic liver cancer, our cooperative study group carried out a randomized clinical trial comparing intra-arterial chemotherapy alone and intra-arterial chemotherapy combined with hyperthermia. Patients were treated with combined chemotherapy of epirubicin (EPIR), mitomycin C (MMC), 5-fluorouracil (5-FU), by hepatic infusion using a subcutaneously implanted reservoir. Hyperthermia (8MHz radiofrequency) was usually performed for 40-60 min every week, and intra-arterial chemotherapy was performed immediately before hyperthermia. Twenty-six patients were registered by telephone contact and allocated at random to groups treated with either intra-arterial chemotherapy alone (14 patients) or combination therapy (12 patients). The response rate was 7% in the chemotherapy alone group (1 PR among 14 evaluable patients), and 40% in the combination therapy group (4 PR among 10 evaluable patients). Our results suggest that intra-arterial chemotherapy combined with hyperthermia is a useful modality for the treatment of metastatic liver cancer.

Adult↗
PubMed1995

[Role of nitric oxide in tachykinin-induced increase in Cl diffusion potential difference of rabbit tracheal mucosa].

To examine the effect of tachykinins on Cl secretion across tracheal mucosa and the possible contribution of nitric oxide (NO) formation to their actions in vivo, we measured Cl diffusion potential difference (Cl-PD) with a high-impedance voltmeter in the presence of amiloride. Superfusion of each neurokinin A (NKA) and substance P (SP) increased Cl-PD in a concentration-dependent fashion, whereas neurokinin B (NKB) had no effect, with the rank order of potency being NKA > SP >> NKB. The tachykinin-induced increase in Cl-PD was inhibited by the NO synthase inhibitor NG-nitro-L-arginine methylester (L-NAME), an effect that was reversed by L-arginine but not by D-arginine. These results suggest that tachykinins increase Cl secretion across rabbit trachea from the submucosa toward the lumen via stimulation of NK2 receptors and that NO formation may be involved in at least part of this process.

Animals↗
PubMed1995

Blue light-emitting diode built-in contact lens electrode can record human S-cone electroretinogram.

PURPOSE: To develop a contact lens electrode with a built-in blue light-emitting diode (LED) to record the electroretinogram from short-wave sensitive-cone (S-cone) electroretinogram (ERG) in humans. METHODS: The ERG was recorded using the blue LED (450 nm) built-in electrode under a yellow background illumination from a slide projector in five normal subjects, a patient with blue cone monochromatism, and a patient with fundus albipunctatus. For comparison, the ERG was also recorded using a yellow LED (566 nm) built-in electrode under the same background illumination in the normal subjects. RESULTS: The amplitude and the peak time of the b-wave recorded with 3-Hz blue stimuli were 5.1 to 12.4 microV and 63 to 68 msec, respectively in normal subjects, 7.3 microV and 65 msec in the patient with blue cone monochromatism, and 11.4 microV and 65 msec in the patient with fundus albipunctatus. Normal subjects responded to neither 30-Hz blue stimuli nor 3-Hz yellow stimuli. CONCLUSIONS: Results indicate that the b-wave recorded with 3-Hz blue stimuli is elicited from the S-cone system. This simple technique is useful for testing the human blue cone system.

Adult↗
PubMed1995

[Anterior chamber inflammation after cataract intraocular lens surgery in postvitrectomy eyes].

We determined the anterior chamber inflammation by means of laser flaremetry after cataract intraocular lens surgery for the nuclear cataract that developed after vitrectomy. Cases (38 eyes of 36 patients) were divided into the following four groups according to their pathologies indicating vitrectomy; proliferative diabetic retinopathy (PDR, 14 eyes), idiopathic posterior vitreous membrane syndrome (PVMS, 9 eyes), rhegmatogenous retinal detachment (RRD, 8 eyes), and branch retinal vein occlusion (BRVO, 7 eyes). Mean intervals between the two surgeries were 31, 23, 27 and 18 months, respectively. Phacoemulsification and aspiration through the self-sealing sclerocorneal wound and intraocular lens implantation in the bag were performed. The mean flare count was already high before the cataract surgery in all groups. Postoperative course of mean flare count was an overlap of two waves, one (P1) with a peak on day 1 followed by rapid decrease and the other (P7) with a peak on day 7 followed by slow decrease. Peak values of P1 were higher than the day 1 value after cataract surgery without fundus pathology, and higher in PDR and BRVO groups than in PVMS and RRD groups. P7 was like that observed after vitrectomy and there was close similarity among the groups.

Aged↗
PubMed1995

[Methods of determination of porphyrins and their precursors--introduction of analytical methods for porphyrin metabolites].

Contrary to the belief that porphyrias are of relatively rare occurrence, there has recently been a rapidly increasing number of reported cases of the disease apparently as a result of growing interest of clinicians, the advance of biochemical and molecular biological research works and improvement and refinement of diagnostic techniques. Unfortunately, however, it is an unquestionable fact that porphyrias are not infrequently still misdiagnosed. Detection and identification of the porphyrias constitutes an important step in the treatment of the diseases and, accordingly, determination of porphyrins and their precursors is of paramount therapeutic importance. While porphyrins are normally detected in small amounts in healthy individuals, these compounds are excreted in large quantities in the urine or feces or both in hereditary and chemical porphyrias, owing to inborn errors in heme biosynthesis and in hemolytic anemia, as a result of excessive production, resulting from increased heme biosynthesis. It follows that determination of porphyrins and their precursors in clinical materials is of pivotal importance in establishing the diagnosis of porphyrias. This paper reviews the methods of determination of porphyrins and their precursors in clinical materials and techniques employed for this purpose.

Aminolevulinic Acid↗
PubMed1995

[Porphyrins in urine, blood, and feces and delta-aminolevulinic acid and porphobilinogen].

The analyses of porphyrins and their precursors in urine, blood and feces are essential for establishing the diagnosis of hereditary porphyrias and for screening for occupational or industrial disease caused by exposure to lead dust or fumes. While porphyrins can easily be separated and extracted by the solvent-extraction method or various chromatographic procedures and provide information of diagnostic importance, high-performance liquid chromatography can now be best used for the separation and determination of porphyrins for differential diagnostic purposes. Where there is need for making a definite diagnosis, determination of activities of enzymes involved in the heme biosynthesis in erythrocyte or lymphocyte and/or genetic diagnosis would be the most reliable way of addressing the problem. These diagnostic techniques, however, are based on highly specialized scientific knowledge and discipline and not suitable for general use. In this paper, a description is given of the most widely used biologic diagnostic criteria and the diagnostic technique underlying them in current use.

Aminolevulinic Acid↗
PubMed1995

[Porphyrin-synthesizing enzymes in erythrocyte].

There are 4 different porphyrin-synthesizing enzymes, i.e. delta-aminolevulinic acid dehydratase, porphobilinogen deaminase, uroporphyrinogen III synthase and uroporphyrinogen decarboxylase, in red blood cells. Determination of activities of these enzymes is of great help in clarifying the hemopoietic mechanism operating in bone marrow erythroblasts, as well as, in establishing the diagnosis of or early detection of acute intermittent porphyria, congenital erythropoietic porphyria, delta-aminolevulinic acid dehydratase deficiency porphyria, porphyria cutanea tarda and lead poisoning. This paper presents a summary of the techniques for the measuring, diagnostic criteria for and clinical implications of activities of these 4 enzymes in red blood cells.

Clinical Enzyme Tests↗
PubMed1995

[Urinary porphyrin screening tests].

While it is known that urinary porphyrins are invariably increased in all varieties of porphyrias, no analytical device, color-developing solution or test paper for specific use in quantifying porphyrins is as yet available. Determination of porphyrins, requiring highly specialized technical skill and experience, is now being done, only in a limited number of special laboratories. For this reason, porphyrias in so many instances are detected late in the course of disease and few physicians are aware of the necessity for making porphyrin measurement. Of greatest importance in the diagnosis of porphyrias is the urinary screening test for porphyrins and screening tests reported to date, though not in widespread use, include those that make use of spectrum, argon ion laser, thin-layer chromatography and ion exchange column chromatography. In this paper, an account is given of three methods currently in most extensive use, i.e. the Schwartz method, Brugsh-Fischer method and high-performance liquid chromatography method, and microplate fluorescence technique, recently developed by the author.

Chromatography, High Pressure Liquid↗