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Biomedical subjects

M Komatsu

Publications and source records attributed to M Komatsu.

At least 343 records · Page 19Linked to original sources
PubMed1982

[Application of the peripheral leukocyte cytofluorogram to diagnosis and treatment of hematopoietic tumors--with special reference to its effectiveness in malignant lymphoma].

In 1970 Adams and Kamentsky reported on a technique for classifying human peripheral leukocytes into three groups-lymphocytes, monocytes and granulocytes using supravital staining with a solution of acridine orange, and analyzing the resulting cell suspension with the Cytofluorogram. For the purpose of clinical application of flowcytometry, we investigated the clinical significance of this cytofluorogram of peripheral leukocytes. Cytofluorograms showed characteristic pattern in such hematopoietic malignancies as acute leukemias, chronic leukemias and malignant lymphomas, and turned out to be applicable to the diagnosis of these diseases. Especially in malignant lymphoma, cytofluorogram showed either normal or abnormal pattern. An abnormal cytofluorogram indicated poor prognosis, and an abnormal pattern of cytofluorogram became normalized after chemotherapy against lymphoma. Interestingly, those patients who showed in abnormal pattern relapsed in a short period, even when they were though diagnosed in remission. These data suggest that cytofluorograms are useful in diagnosing, predicting relapse and evaluating therapeutic effects for the treatment of malignant lymphoma. We hope that the analysis of cytofluorogram of peripheral leukocytes in lymphoma will provide a new benefit for further investigation of this disease.

Acridine Orange↗
PubMed1981

[Clinical evaluation of cefotiam in internal medicine, with special reference to infectious disease associated with hematological disorders (author's transl)].

Clinical evaluation of cefotiam (panspolin), a new cephem antibiotics, was performed in the infectious disease associated with hematological disorders and in the respiratory system. In hematological dis orders, 40% of good and 25% of fair results were obtained in clinical effects. In respiratory infections, however, 92% of good results were obtained. Opportunistic infection due to Gram-negative bacilli are often experienced in patients with leukemia. Since cefotiam has sufficient bacteriocidal effects in broad spectrum, it would be a good therapeutic agent against infectious diseases associated with hematological disorders.

Adolescent↗
PubMed1980

Actin-induced local conformational change in the myosin molecule. III. Reactivity of S2 thiol and DTNB-reactive thiols of porcine cardiac myosin.

The reactivity of thiol groups in cardiac myosin obtained from porcine ventricles was compared with that in rabbit skeletal myosin. 1. The specific thiol group, S2, of cardiac myosin became reactive to N-ethylmaleimide (NEM) upon the addition of Mg2+-ATP or Mg2+-ADP, as in the case of skeletal myosin, although to a lesser degree. Enhancement of the reactivity of S2 resulting from cooperative interaction between F-actin and Mg2+-ATP, but not Mg2+-ADP, which was first found in skeletal myosin, was also observed in cardiac myosin. 2. The total number of 5,5'-dithiobis(2-nitrobenzoate) (DTNB)-reactive thiols decreased in the presence of Mg2+ADP and decreased further in the presence of Mg2+-ATP for both myosins, in contrast to the concomitant increase in the reactivity of S2. The decrement was smaller in cardiac myosin than in skeletal myosin. 3. The DTNB-reactive thiols were classified into three groups with respect to their reactivity. The third or most slowly reacting thiol group, was found to be remarkably susceptible to Ca2+ at physiological concentrations (10(-8)-10(-6) M) in the presence of Mg2+-ADP for cardiac myosin, but in striking contrast, in the presence and relatively long-lived intermediate of myosin ATPase, the structure of which is sensitive to Ca2+, may be different in cardiac and skeletal myosins.

Actins↗
PubMed1979

Development and genetic differences of complement activity in rabbits.

The ontogeny of haemolytic complement in rabbit serum and the genetic differences of the activity in five strains of adult rabbits were investigated by single radial haemolysis in gel and a microtiter method with purified complement components and the appropriate haemolytic intermediate cells. The haemolytic complement activity was found as early as the 15th day of foetal life, and increased with age reaching approximately adult level by the 120th day of life. Marked strain differences in both total haemolytic activity and C3 levels of adult rabbit sera were observed. The repeatability of haemolytic activity for an individual serum taken at different times was higher than that for C3 level was observed. An inherited complement deficiency, due to the lack of C6, was found in a strain of Angora rabbits. The genetic studies confirmed that this complement defect was transmitted as an autosomal recessive trait.

Aging↗
PubMed1978

Use of nitrogen mustard N-oxide for the fixation of electron microscopic tissues.

Nitrogen mustard N-oxide was tried for the fixation of tissue for electron microscopy. A fixative consisting of 1% nitrogen mustard N-oxide, 1% glutaraldehyde and 1% paraformaldehyde buffered at pH 7.4 followed by 1% OsO4 buffered at pH 7.4 was found useful for the tissues examined: thyroid, anterior pituitary, adrenal gland and oviduct of mice. If the tissues are fixed and the sections are stained with uranyl acetate and lead acetate doubly, the follicle colloid, colloid droplets, and secretory granules containing thyroglobulin in the thyroid become higher in electron density. The cisterna of the maturing face of the Golgi apparatus, secretory granules, ribosomes, nucleolus and chromatin in the cells examined are extremely electron dense. Tubular elements of smooth endoplasmic reticulum in the adrenal cortical cell and microtubules in all the cells examined are also well preserved. The fixative containing nitrogen mustard N-oxide is useful also for cytochemistry. Using tissue fixed by this method and stained en bloc by uranyl acetate, the noradrenaline and adrenaline cells in the adrenal medulla are clearly distinguished by light microscopy.

Adrenal Medulla↗
PubMed1978

Electron-microscopic studies on the development and aging of the oviduct epithelium of mice.

The fine structure of the oviduct epithelium of the newborn to the old mouse was studied with an electron microscope. Just after birth, epithelial cells lining the ampulla of the mouse oviduct are simple columnar in shape and of one type in fine structure. They contain numerous free ribosomes, an extremely poor rough endoplasmic reticulum, and a small Golgi complex. In the 3-day-old mouse, the epithelial cells are differentiated neither into ciliated nor secretory cells, and are characterized by the appearance of many autolysosomes and a solitary cilium. The ciliary cells differentiates 5 days after birth. Ciliogenesis is frequently observed at 5-10 days. The important role of the fibrous granules for ciliogenesis and that of the Golgi apparatus for membranogenesis of the cilia are described and discussed. The secretory cell having mucous secretory materials is differentiated at 23 days. In the epithelial cell lining the ampulla of the aged (22 to 24-month-old) mouse oviduct, large autolysosomes and vacuoles 2-6 micrometer in diameter occur in the ciliated cell, though cilia and other cell organelles are well preserved. In the old mouse the secretory granules almost disappear and the rough endoplasmic reticulum is strikingly dilated in the secretory cell. No features showing the transformation between the secretory cell and the ciliary one are seen in the mouse oviduct.

Aging↗