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Biomedical subjects

M Kitagawa

Publications and source records attributed to M Kitagawa.

At least 433 records · Page 24Linked to original sources
PubMed1990

Stimulatory effects of bombesin on plasma trypsin release and exocrine pancreatic secretion in dogs.

We examined the effect of bombesin on plasma trypsin release and exocrine pancreatic secretion in dogs. Bombesin significantly increased plasma immunoreactive trypsin (IRT). Atropine significantly inhibited the response of plasma IRT to bombesin. Pancreatic trypsin secretion was also increased by bombesin, as well as bicarbonate and protein outputs. Atropine failed to inhibit pancreatic trypsin secretion. In conclusion, bombesin has a stimulatory effect on plasma trypsin release mediated by a cholinergic mechanism and different from pancreatic secretion.

Amylases↗
PubMed1990

Serum Lp(a) lipoprotein concentrations of Japanese patients with coronary heart disease.

The serum Lp(a) lipoprotein concentration in 103 healthy control subjects was determined by ELISA (TintElise Lp(a) kit, Biopool, Umea, Sweden). The distribution of Lp(a) in the controls was highly skewed (mean 132, S.D. 109, median 99 mg/liter), which is similar to the results previously reported. Serum Lp(a) level in 104 subjects who underwent coronary angiography was also measured. These subjects were divided into two groups: 59 cases of stenosis(-) and 45 of stenosis(+) groups. Lp(a) level of the stenosis(+) group (mean 235, S.D. 197, median 159 mg/liter) was significantly higher than that of the stenosis(-) group (mean 156, S.D. 133, median 123 mg/liter) and of the controls (Wilcoxon test, p less than 0.05 and p less than 0.01, respectively). Further, stenosis(+) group was divided into three subgroups: 20 with single vessel disease, 13 with double vessel disease and 12 with triple vessel disease subgroups. Lp(a) level was correlated with the number of stenotic coronary vessels (Spearman's rank correlation coefficient, p less than 0.05). These results suggest that Lp(a) may play an important part as a risk factor for coronary heart disease.

Adult↗
PubMed1990

Effect of dietary proteins and/or their digestive products on intestinal taurocholate absorption.

[14C]Taurocholate was orally administered to rats together with a definite amount of either casein- or soy protein-based diet and its postprandial movement along the digestive tract was investigated. A difference was observed between both dietary groups in intraluminal transit as well as mucosal accumulation of [14C]taurocholate in the ileum; namely the soy protein intake led to a decrease in the bile acid incorporation into the ileal mucosa relative to the casein intake, although raising its intraluminal stay. In addition, the digestive products from these and other food proteins by pepsin-pancreatin digestion (peptides with molecular weights of more than 1,000) were examined for their inhibitory effects on in vitro absorption of taurocholate with ileal everted sacs. As the digestive product affinity for taurocholate increased, the rate of taurocholate absorption decreased. It thus seems likely that a food protein more abundant in hydrophobic peptides following intraluminal digestion adsorbs much more bile acids in the gut, thereby disturbing their intestinal absorption.

Animals↗
PubMed1990

[201T1 single photon emission computed tomography in the diagnosis of lung cancer].

201T1 SPECT was performed in 84 patients with suspected lung cancer. Both early and delayed scans demonstrated abnormal accumulation in 67 of 68 malignant pulmonary lesions and in nine of 18 benign conditions. All of 64 lung cancer were clearly visualized. Delayed ratio (uptake ratio of the lesion to the normal lung on delayed scan) and retention index (degree of retention in the lesion) were helpful to differentiate malignant from benign lesions. This method was also useful to evaluate mediastinal involvement from lung cancer.

Adenocarcinoma↗
PubMed1990

[A case of primary leiomyosarcoma of the lung].

We reported a case of 66-year-old female with leiomyosarcoma of the right lower lobe of the lung with the complaint of abnormal shadow of the chest roentgenogram. On December 2, 1987, partial resection was performed and the tumor was totally removed. Histological examination revealed primary leiomyosarcoma of the lung. By electron microscopic observation, pinocytotic vesicle was not observed, but myofilaments were observed in the cytoplasm.

Aged↗
PubMed1990

[Metastatic Grawitz's tumor to the cauda equina: case report].

A case of Grawitz's tumor metastasizing to the cauda equina is presented. A 51-year-old male was hospitalized due to severe low back pain radiating to the left lower extremity. Neurological examination showed only hyporeflexia of the left patella reflex and positive Lasegue's sign. MRI showed intradural mass at the L4 level. Preoperatively, we diagnosed a cauda equina tumor. A laminectomy of both L3 and L4 was performed, and total removal of the cauda equina tumor was performed. Microscopically, the tumor cells were large, the appearance of the cytoplasm ranging from optically clear with sharply outlined boundaries, to deeply granular, with many transitional forms. These histological findings were typical findings of Grawitz's tumor, and were the same as those of this patient's renal tumor. Finally, we diagnosed Grawitz's tumor metastasizing to the cauda equina. Metastatic cauda equina tumor from outside the central nervous system is very rare and only 7 cases have been reported. This case is the first one of Grawitz's tumor spreading to the cauda equina.

Carcinoma, Renal Cell↗
PubMed1990

A new technique for one-stage radical eradication of long-standing chronic thoracic empyema.

The surgical treatment of chronic long-standing thoracic empyema remains a challenging subject. A simple technique that consumes less operative time, accompanying less bleeding, and that enables eradication of the disease in one stage was devised by the senior author. The technique requires only three major steps: (1) decortication limited to the parietal sides of the peel's sac, (2) cleansing the empyemic cavity, and (3) drainage. To date, six patients have been treated by this method; complete cure was achieved in all. Neither death nor recurrence of the disease has been noted during the follow-up period averaging 24.5 months. The mean operative time was 3.06 hours, mean bleeding volume was 1262 ml, and the mean hospital stay period was 1.14 months. The methods and results of the technique are discussed.

Aged↗
PubMed1989

[Giant renal cell carcinoma: a case report].

Though renal cell carcinoma is a fairly common disease, it is extremely rare to encounter a case providing a resected mass as large as over 2,000 g in weight. We report a case in which a giant 2,200 g tumor was resected after renal arterial embolization and interferon treatment for reduction of its size. A forty-three-year-old woman was first seen at a certain hospital with a chief complaint of fever and right flank pain. Under suspicion of right renal tumor, nephrectomy by extraperitoneal flank incision was attempted. However, the tumor was too large to be resected. After transfer to our department, she was treated by renal arterial embolization with absolute ethanol and steel coil and by the administration of interferon (rIFN-alpha A). As the tumor shrank to 77% of its original size on CT measurements and became mobile, transperitoneal nephrectomy was performed. The resected mass was as large as 12 x 12 x 21 cm. Pathologically, it was a clear cell type tumor showing mucinous degeneration and hyaline degeneration of tumor cells. Interferon used amounted to as high as 864 x 10(6) units, but no particular side effect occurred. No recurrence has been detected so far.

Adult↗
PubMed1989

Chronic alcoholism and evolution of pain and prognosis in chronic pancreatitis.

To evaluate the influence of chronic alcoholism on clinical features of chronic pancreatitis in Japan, pain evolution, pancreatic insufficiency, and long-term prognosis were studied by comparing chronic alcoholic pancreatitis (N = 88) with idiopathic pancreatitis (N = 67). The 155 patients with known course of the disease over three years were followed-up further for five more years, and pain evolution was evaluated once at the start and once at the end of the follow-up period. At the time of diagnosis, severe pain (59 vs 33%, P less than 0.001), pancreatic calcification (63 vs 31%, P less than 0.001), advanced exocrine pancreatic insufficiency (72 vs 60%, NS), and overt diabetes (48 vs 17%, P less than 0.001) were more common in alcoholic than in idiopathic pancreatitis, respectively. Pain evolution was similar in both pancreatitis, and the pain decreased with time. The rate of abstinence was higher in groups with pain relief than without in alcoholic pancreatitis. Cumulative mortality rate during the five years was higher in alcoholic than idiopathic pancreatitis (26 vs 10%, P less than 0.01). These results suggest more favorable evolution of the disease can be expected by abstinence from alcohol.

Adult↗
PubMed1989

Serum insulin-like growth factor II in chronic liver disease.

Insulin-like growth factor II is secreted primarily by the liver and is reported to be transcribed in many primary hepatocellular carcinoma (PHC) cell lines. We have studied diagnostic significance of serum IGF-II in chronic liver diseases using specific enzyme immunoassay. Serum IGF-II levels (mean +/- SE) were decreased in chronic hepatitis (538 +/- 51 ng/ml; N = 29), liver cirrhosis (427 +/- 45; 50) and PHC (260 +/- 41; 17) compared to controls (830 +/- 49; 57). Serum IGF-II was not different from controls in any of nonhepatic diseases such as diabetes (1032 +/- 97; 19) pancreatic cancer (1413 +/- 282; 8), chronic pancreatitis (999 +/- 126; 17), peptic ulcer (1186 +/- 43; 11), irritable bowel syndrome (1002 +/- 109; 12), gastrointestinal tract cancer (1250 +/- 216; 21) and chronic renal failure (733 +/- 135; 14). In liver diseases serum IGF-II showed a significant correlation with liver function test (negative with retention of indocyanine green and total bile acids; positive with albumin, thrombo-test, and cholinesterase). These results suggest that serum IGF-II reflects a reduced production of IGF-II in the liver and that it can be an index for the residual capacity of liver function.

Adult↗
PubMed1989

Scirrhous carcinoma of the stomach: a clinical and pathological study of 106 surgical cases.

Clinical and pathological characteristics of scirrhous carcinoma of the stomach were studied in 106 cases treated by gastrectomy between 1973 and 1983. The male to female ratio was 0.58. The percentage of scirrhous carcinomas to all gastric carcinomas resected in the same period was three times higher in females than males. The age distribution of the patients suggested that there were two peaks in the forties and sixties in the male, and in the thirties and fifties in the female. The incidence of scirrhous carcinoma in all types of gastric carcinoma was significantly higher in the twenties, thirties and forties compared to the lowest incidence in the seventies. In the female group the primary lesion had a tendency to be adjacent to the fundic gland area and to avoid intestinal metaplasia. In the male the opposite was recognized. Cancer nests with single cells or only several cells were common in this type of carcinoma. These findings suggest that there might be two biologically different scirrhous carcinomas both in the male and the female, the appearance of single carcinoma cells might be favored by female sex hormones and young ages, and not only the original gastric mucosa but also mucosa with intestinal metaplasia could be precursors of single carcinoma cells.

Adenocarcinoma, Scirrhous↗
PubMed1989

Enzyme immunoassay for serum pancreatic lipase in the diagnosis of pancreatic diseases.

To evaluate the diagnostic utility of serum immunoreactive lipase (IRL), serum lipase was determined using an enzyme immunoassay and a turbidimetric method along with total serum amylase in 41 healthy controls, 76 patients with pancreatic disease and 60 with nonpancreatic disease. Serum IRL was elevated in 12 of 13 patients with acute pancreatitis, 12 of 44 with chronic pancreatitis and in 12 of 19 with pancreatic cancer. The IRL was low in 9 of the 44 patients with chronic pancreatitis, which coincided with advanced exocrine pancreatic insufficiency. Overall sensitivities in pancreatic diseases were 59% for serum IRL, 38% for turbidimetric lipase and 51% for amylase, specificities in healthy controls and nonpancreatic diseases were 80% for serum IRL, 86% for turbidimetric lipase and 88% for serum amylase. Serum IRL determination is useful for diagnosis in pancreatic diseases when compared with the conventional determination of serum lipase.

Adult↗
PubMed1989

Bone marrow analysis of the myelodysplastic syndromes: histological and immunohistochemical features related to the evolution of overt leukemia.

Bone marrow trephines from 31 patients with an initial diagnosis of myelodysplastic syndromes (MDS) were examined and analyzed histologically and immunohistochemically. In those cases terminating in overt leukemia (6/31, 19%), the number of bone marrow mast cells was significantly reduced, compared with those which did not evolve to overt leukemia. The bone marrow lymphoid cells that may participate in immunosurveillance against the proliferation of blast cells were also significantly reduced in cases terminating in overt leukemia. However, S-100 protein-positive cells, which include histiocytes and suppressor T-cells, were increased in cases terminating in overt leukemia. The results indicated that examination of the bone marrow to determine the proportions of mast cells and lymphoid cells which may be involved in host defense systems may be useful in predicting the evolution to overt leukemia in MDS. In the present series, patients with a hypocellular marrow (5/31, 16%) did not progress to overt leukemia and had a significantly lower bone marrow reticulin content, a significantly lower megakaryocyte count, a relatively higher mast cell count and a significantly higher lymphoid cell count than those with a normocellular or hypercellular marrow. These findings may reflect the initial features of MDS or, possibly, that hypocellular MDS is an independent entity with a low potential for blastic proliferation.

Aged↗
PubMed1989

Urinary and serum zinc levels in chronic pancreatitis.

Urinary and serum zinc levels were determined in 51 patients with chronic pancreatitis. Urinary zinc excretion in patients with chronic calcified pancreatitis (832 +/- 111 micrograms/day) (mean +/- SE) but not in noncalcified pancreatitis (684 +/- 65 micrograms/day) was significantly higher than in normal controls (418 +/- 46 micrograms/day). The urinary zinc excretion increased with deterioration of exocrine pancreatic function. Serum zinc levels in advanced pancreatitis (105.9 +/- 4.5 micrograms/100 ml) were significantly higher when compared to the pancreatitis with normal exocrine pancreatic function (91.6 +/- 3.0 micrograms/100 ml), but the difference was less pronounced than for urinary zinc excretion. This may be due to complicating diabetes, which usually lowers serum zinc. Serum zinc and urinary zinc excretion were low in a patient with chronic calcified pancreatitis complicated with a pulmonary abscess and hypoalbuminemia. In conclusion, urinary and serum zinc levels in chronic pancreatitis were increased as a result of exocrine pancreatic dysfunction. Association of diabetes may lower serum zinc, and associated malnutrition depresses both urinary and serum zinc levels.

Adult↗
PubMed1989

Antigenic analysis between BCG and tumor cells by BCG-monoclonal antibodies.

Antigenic relation between Mycobacterium bovis strain BCG and experimental animal tumor cells was analyzed with BCG monoclonal antibodies (BCG-MoAbs). Four BCG-MoAbs of 602, 603, 609, and 612 were successfully established and applied for the antigenic analysis of Meth A, RL male 1, colon tumor 26 of mouse, and line 10 of guinea pig tumor. MoAb 602 showed broad reactivity against all types of tumor cells. BCG antigen(s) was clearly recognized as small granules on the tumor cell surface under the fluorescence microscope, indicating that the animal tumor cells shared the common antigen(s) with BCG.

Animals↗
PubMed1989

[Study of umbilical cord coiling and variable deceleration].

Using cardiotocogram and umbilical arterial gas analysis, the presence or absence and variations of umbilical cord coiling were examined with relation to the incidence of variable deceleration (V.D.) and neonatal asphyxia. The results obtained were as follows. 1) The incidence of V.D. with cord coiling was higher than that without cord coiling (p less than 0.005). The incidence of severe V.D. with cord coiling was also higher than that without cord coiling (p less than 0.005). 2) The mean Apgar scores of cases with cord coiling were lower than that without cord coiling (p less than 0.02). 3) On the umbilical arterial gas analysis, the mean pH of cases with cord coiling was lower than that without cord coiling (p less than 0.05). And the mean pCO2 of cases with cord coiling was higher than that without cord coiling (p less than 0.005). So neonate with cord coiling showed a tendency towards respiratory acidosis in comparison with that without cord coiling. 4) The incidence of neonatal asphyxia with cord coiling was 16.0% (Apgar score less than or equal to 7), 20.0% (pH less than 7.20) and that without cord coiling was 8.8%, 13.2%, respectively. Although there was no statistically significant difference, a tendency was observed towards a higher rate of neonatal asphyxia with cord coiling. 5) The incidence of neonatal asphyxia with severe V.D. was 44.4% (Apgar score less than or equal to 7), 36.1% (pH less than 7.20) and that without severe V.D. was 5.7%, 8.6%, respectively. There was a statistically significant difference (p less than 0.005, p less than 0.005). In addition, the incidence of neonatal asphyxia with repeated V.D. was 35.3% (Apgar score less than or equal to 7), 44.1% (pH less than 7.20) and without repeated V.D. was 3.8%, 6.5%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Asphyxia Neonatorum↗
PubMed1989

Effects of elevated d-alpha(RRR)-tocopherol dosage in man.

A study was conducted to investigate the effects of a megadosage of free RRR-alpha-tocopherol in healthy college student volunteers. Of 19 volunteers, 14 were given daily doses of 600 mg (900 IU) of RRR-alpha-tocopherol for 12 weeks, and the remaining 5 were given identical placebo capsules. The investigation was performed by the single-blind method. Alpha-tocopherol levels were measured in plasma, red blood cells (RBCs), platelets, leucocytes (WBCs), and buccal mucosal cells. Alpha-tocopherol in plasma, RBCs, and WBCs rose, reached a maximum level 4 weeks after commencement of administration, and then remained at a plateau, while platelet and buccal cell levels reached a maximum level after 12 weeks of administration. The maximum levels in all the subjects were 2.5 to 3 times the baseline values. During the study, there were no changes in laboratory values for thyroid, liver, or kidney functions, and coagulation activity (including the vitamin K-dependent Hepaplastin test and PIVKA-II) or immunoglobulin levels. Healthy status continued without any abnormal symptoms, and without any subjective complaints on the questionnaire. In the control group also, no changes occurred during the investigation. Gamma-tocopherol changes were measured in plasma and RBCs. As plasma and RBC tocopherol levels rose after administration, the isomer levels were suppressed in both plasma and RBCs.

Adult↗