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Biomedical subjects

M Kist

Publications and source records attributed to M Kist.

At least 73 records · Page 4Linked to original sources
PubMed1988

Antibody response of patients against a 120 kDa surface protein of Campylobacter pylori.

Campylobacter pylori strains were isolated and serum samples were obtained from 63 patients. Immunoblots of 52 patients sera using their own isolates as antigen showed a 120 kDa band, which was missing in the other 11 isolates and the respective sera. This band was not detected in other Campylobacter species. Effects of trypsin treatment of bacteria and absorption of sera by live organisms suggest a C. pylori-specific surface protein.

Antibodies, Bacterial↗
PubMed1988

Immunological reaction of guinea-pigs following intranasal Mycoplasma pneumoniae infection and immunization with the 168 kDa adherence protein.

Humoral responses to Mycoplasma pneumoniae proteins, especially the 168 kDa protein, were demonstrated by Western blotting in sera and bronchial washings of all groups of infected or immunized guinea-pigs. However, infection was not prevented by these local and systemic antibodies. Hilar lymphocytes of infected and immunized guinea-pigs were stimulated in vitro by sonicated M. pneumoniae antigen and by the 168 kDa protein. Stimulation was significantly lower in animals which had been infected twice or had been preimmunized and challenged by infection. Histologically the most severe lesions were seen in the twice-infected group followed by the preimmunized group which was subsequently infected.

Animals↗
PubMed1987

Cryptosporidia in immunocompetent patients with gastroenteritis.

In a prospective study Cryptosporidium spp. were detected in nine immunocompetent patients (0.6%). The frequency was remarkably higher in children in the 0 to 14 year age group (1.6%), surpassed only by Salmonella spp. and Campylobacter spp. The predominant clinical features were watery diarrhea, abdominal pain, and vomiting. Comparison of the epidemiological data of the patients with those of a control group showed that contact persons with enteritis and drinking of unpasteurized milk were significantly associated with cryptosporidiosis.

Adolescent↗
PubMed1986

[Who discovered Campylobacter jejuni/coli? A review of hitherto disregarded literature].

Non culturable spiral form bacteria were noted in 1886 by Theodor Escherich. He found them in stool specimens and large intestinal mucous associated with diarrhoea in neonates as well as in kittens. In the following years till the end of the century a number of mainly german language publications appeared, describing the occurrence of such "spirilla" in cases of "cholera-like" and "dysenteric" disease. These organisms were found mainly in the colon or associated with mucous in diarrheal stool specimens. Growth on solid medium was unsuccessful, although living bacteria could be kept in liquid culture medium for a few days. The following points suggest that the microorganisms described were probably Campylobacter ssp: typical morphology association with enteritis in neonates, infants and kittens failure to grow on solid medium despite microscopic detection and the fact that to date no other bacteria with comparable morphology have been associated with human enteric infections.

Animals↗
PubMed1985

[Yersinia pseudotuberculosis infections. Etiologic clarification using fecal cultures].

Isolation of the causative organism in stool proved the aetiology of Yersinia pseudotuberculosis in two patients. In a 12-year-old boy the clinical picture was of appendicitis. After appendicectomy the histological findings provided the diagnosis. The second patient, a 2-year-old girl, had protracted enteritis, completely cured by a course of cotrimoxazole. Culturing of Y. pseudotuberculosis from stool has previously been very rare. But new methods of culturing and other recent advances provide a better chance of success. Isolation of the organism in the acute stage of the disease should be attempted both from stool and from any biopsy material obtained during operation.

Adolescent↗
PubMed1985

Mycoplasma pneumoniae induces cytotoxic activity in guinea pig bronchoalveolar cells.

Precultured guinea pig alveolar macrophages (AM) and freshly harvested alveolar cells (FHAC) activated by interaction with Mycoplasma pneumoniae were cytotoxic for xenogeneic 75selenomethionine-labeled tumor target cells. Phagocytosis of whole opsonized or nonopsonized M. pneumoniae cells was more effective in eliciting cytotoxicity than uptake of sonicated microorganisms. The addition of living mycoplasma cells to the assay system enhanced the cytotoxic effect considerably. Target cells were significantly more susceptible to the cytotoxic action of phagocytes if they were coated with mycoplasma antigen or cocultured together with M. pneumoniae. The activation of the phagocytes could be inhibited by 2-deoxy-D-glucose but not by antimicrobial substances suppressing mycoplasma protein synthesis. It was accompanied by 51Cr release without detectable signs of cell damage. The supernatants of activated cells were cytotoxic for approximately 24 h. Inhibition, release, and cytotoxic activity indicate the necessity of an intact metabolism of the effector cells and suggest a secretion of cytotoxic substances.

Animals↗
PubMed1983

[Campylobacter infection in children].

Enteritis by Campylobacter jejuni/coli is by far the most frequent clinical entity of campylobacter-infection. It may be compared to salmonella-enteritis as for its epidemiologic importance. The course of the disease may be dramatic, but can be successfully treated as soon as the pathogen is known. In prematures and newborn babies infections with Campylobacter fetus ssp. fetus are life-threatening and early diagnosis is of life-saving importance. Five observations of campylobacter-enteritis in children aged 2, 8, and 10 years are reported. They were characterized by high fever frequent loose, watery stools with blood, leucocytosis or leucopenia and elevated BSR. All children became asymptomatic with symptomatic treatment, without specific antibacterial therapy. The importance of campylobacter-infections, their diagnosis and course of disease in children are discussed.

Anti-Bacterial Agents↗
PubMed1982

Release of Mycoplasma pneumoniae substances after phagocytosis by guinea pig alveolar macrophages.

Antibody-opsonized Mycoplasma pneumoniae cells with various radioactive markers were sedimented onto monolayers of guinea pig alveolar macrophages (AM). After 2 h of incubation, about 50% of the activity of [3H]palmitate-labeled mycoplasmas was associated with AM. Nonspecific attachment of the opsonized mycoplasmas to AM-free plastic surface areas was negligible. The occurrence of phagocytosis was proven by electron microscopy and monitoring of AM surface-bound antigen by 125I-labeled F(ab)2 fragments. The activity of [3H]palmitic acid-labeled mycoplasmas was only slowly released into the supernatant. About 55% of the activity remained AM-associated up to 70 h after phagocytosis. After phagocytosis of [3H]thymidine-labeled cells, about 70% of the radioactivity found non-precipitable by trichloracetic acid. 3H-amino acid-labeled protein was released to 50% within 8 h. Supernatants and AM were tested for M. pneumoniae antigen with enzyme-linked immunosorbent assay. Considerable amounts of antigenically active material could be found in the supernatant within 8 h. This antigen was totally inactivated by heat (80 degrees C). Trypsin treatment (1 mg/ml, 10 min) reduced the antigenicity by 80%. The results suggest a selective release of microbial material after phagocytosis.

Animals↗
PubMed1982

[Salmonella landwasser: a new serotype of Salmonella subgenus I (3,10:z:z6)].

A new Salmonella serotype (3,10:z:z6) was isolated from a fecal specimen of a lizard (Lacerta viridis) imported from Northern Italy. The strain was accepted as a new Salmonella, Subgenus I, on January 11, 1980, by Prof. Le Minor, International Salmonella Centre Paris. The organism was introduced into the Kauffmann-White-Scheme, Supplement XXIII as S. landwasser in referring to the first isolation in a Veterinary Research Institut in Landwasser, a suburb of Freiburg (FRG).

Animals↗
PubMed1981

Phagocytosis and complement action.

THe interaction of pathogenic mycoplasmas with two components of the "nonspecific" defence system is influenced by the lack of a cell wall and perhaps the tight attachment to host cells. On mycoplasmas, complement can act directly on the parasite membrane surface and cause damage comparable to the effects on animal cells, namely lysis and death. Results with Mycoplasma pneumoniae suggest a direct activation by this species of the complement sequence without participation of homologous antibody. Activation may occur via the alternate pathway and perhaps even by direct triggering of the classical pathway. The action results in rounding, opsonization and death of some or all of the cells. Since complement components have been found in bronchial washings, this direct activation may be of some importance in the first stages of infection. Furthermore, it sufficiently explains the fact that M. pneumoniae is found only on the surface but not in the deeper tissues of the respiratory tract even in severe infections. The interactions of mycoplasmas with phagocytes, one of the first lines of defence against infection, is of considerable interest. Mycoplasmas seem to be relatively resistant to phagocytosis as long as they are not opsonized. They have been observed attaching to macrophage surfaces without being ingested. Addition of homologous antibody triggered immediate engulfment and intracellular killing. The aspects of interaction of phagocytes with mycoplasmas are even more interesting because in most cases the mycoplasmas which have to be phagocytized are attached to the surface of other tissue cells. This raises the problem of secondary damage caused by the enzymes of the phagocytizing cell. Our knowledge about the mycoplasma-phagocyte-interaction is scarce and the experimental approaches are difficult.

Animals↗
PubMed1980

[Spread of Yersinia enterocolitica infection within a hospital (author's transl)].

Infections linked in time and space with Yersinia enterocolitica (serovar 0 : 9, biotype 2, lysotype X3) were observed in 6 cases within one university hospital. After a hospital epidemic in Finland in 1973 (involving 7 persons) this is the 2nd such observation. The spread was demonstrated in two areas. The probable source of infection of one patient group (n = 3) was a 48-year-old dialysis patient admitted with a febrile condition without enteritic symptoms. Pathogens could be demonstrated in his faeces by direct culture, in two contact persons cultivation was possible only with cold enrichment. Blood cultures of the first patient were negative. At about the same time 3 further infections with Yersinia enterocolitica of the same characteristics were observed in hospital personnel from different units. Frequent exchange of staff and patients among affected wards increases likelihood of cross-infection. A dialysis nurse with Yersinia arthritis was the possible link between patients and personnel. Transference of infection from one person to another can be assumed in the above cases.

Adult↗
PubMed1980

Characterization of pili associated with Escherichia coli O18ac.

A strain of Escherichia coli O18ac isolated from the stool sample of a patient with diarrhea was found to agglutinate human erythrocytes. From the results presented it is suggested that this hemagglutination is mediated by pili. Isolated pilus preparations agglutinated human erythrocytes, whereas pilus-negative mutants did not. The serological and chemical analyses indicate that the pili associated with E. coli O18ac are distinct from other types found with E. coli.

Agglutination Tests↗
PubMed1980

[Yersinia enterocolitica: the influence of incubation temperature on antibiotic susceptibility, beta-lactamase activity and growth characteristics (author's transl)].

The influence of incubation temperature (37, 30 and 22 degrees C) on antibiotic susceptibility, beta-lactamase activity and growth characteristics was studied on 43 unselected strains of Y. enterocolitica (Serovar O:3 and O:9) freshly isolated from cliical specimens. Antibiotic susceptibility was measured by the disc diffusion technique and by a broth dilution test (MIC). Beta-lactamase activity was detected with chromogenic cephacetrile using standard curves prepared for 37, 30 and 22 degrees C. Continuous increase of beta-lactamase activity was found when incubation temperatures were lowered. All strains were found to be resistant by ampicillin and cephalothin at the three temperatues tested. Some strains showed an intermediate susceptibility to carbenicillin in the disc diffusion test. A temperature reduction of 37 to 30 degrees C significantly decreased the inhibitory zone diameters for the beta-lactam antibiotics ampicillin, carbenicillin and cephalothin, but also for other substances like tetracycline, chloramphenicole and cotrimoxazole. This suggests, that the observed decrease is caused by a better growth of Y. enterocolitica at 30 degrees C rather than increased beta-lactamase production. From 30 to 22 degrees C a further decrease in inhibitory zone diameters was only seen with ampicillin and carbenicillin. This seems to be mainly due to the increased B-lactamase activity observed at 22 degrees C. In contrast the resistance to cephalothin was apparently not influenced by this additional beta-lactamase activity. Resistance to cephalothin therefore depends probably more on other, not beta-lactamase-related, factors such as permeability variations of the outer membrane or modifications of binding proteins involved in the peptidolycan biosynthesis. The correlation between beta-lactamase activity at various incubation temperatures and resistance to beta-lactam antibiotics was less pronounced when the broth dilution test (MIC) was applied. Only carbenicillin showed significantly increasing MIC values from 30 to 22 degrees C. All the Y.e. strains investigated could be divided into two groups with respect to their beta-lactamase production characteristics. The first group showed continuously increasing beta-lactamase values at lower incubation temperatures. In the second group generally lower amounts of beta-lactamase values were found and temperature dependence was not observed. On the other hand variations in cell wall permeability, resulting in a diminished accessability of the cell wall bound enzymes must also be considered.

Anti-Bacterial Agents↗