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Biomedical subjects

M Kikuchi

Publications and source records attributed to M Kikuchi.

At least 1,153 records · Page 64Linked to original sources
PubMed1976

Chemical and physical properties of peptidoglutaminase I and II from Bacillus circulans.

Some chemical and physical properties of Peptidoglutaminase I and II have been determined. For example, molecular weight, isoelectric point, intrinsic viscosity, partial specific volume, sedimentation and diffusion coefficient were 89 000 and 105 000, pH = 4.1 and 4.0, 0.042 and 0.065 dl/g, 0.733 and 0.728 ml/g, 5.85 and 5.3 S, 5.32 and 4.57-10(-7) cm2-s-1, respectively. Their amino acid compositions were also determined. Between them, peptidoglutaminanse II was a glycoprotein containing 2 mol of galactose and 11 mol of glucosamine per mol of enzyme. Both enzyme molecules were composed of two subunits with an equal molecular weight. N-terminal amino acids for peptidoglutaminase I were phenylalanine and valine, and leucine for peptidoglutaminase II. Both proteins were immunologically different from each other.

Amidohydrolases↗
PubMed1976

Aging and activity of 5-aminolevulinate hydrolyase in the blood of humans and rats.

Activity of 5-aminolevulinate hydrolyase (delta-aminolevulinic acid dehydrase, ALAD, EC 4.2.1.24) in the blood was determined in relation to age in humans and rats, and the following findings were obtained: (1) In the group which consisted of 47 women (mean age 46, ranged 24 to 62 years) living in a rural district, where the atmospheric lead concentration was 0.5 micron/m3, a significant negative correlation between ALAD activity and age was observed statistically (r = -0.54). However, in the group which consisted of 53 women of similar age group living in an urban district, where the atmospheric lead concentration was 1.9 microng/m3, showed a lower level of the ALAD activity than that of the former group and no correlation was found r = -0.01) between ALAD activity and age. (2) The ALAD activity of intact rats showed a decrease with age. The decrease proceeds rapidly within 8 weeks of age and becomes stable after reaching 25 weeks of age or 300 g of body weight. The value of the activity after 25 weeks of age was about one tenth of that at 5 weeks of age or 100 to 130 g of body weight. (3) Fifteen intact rats weighing 240 to 705 g were divided into three groups according to their body weight and were injected with lead acetate solution equivalent to 1 mg Pb/kg intraperitoneally in order to examine the sensitivity to lead in rats that have variation of ALAD activity by age or by body weight. The ALAD activity of these rats fell to the same level in all groups independently of the activity or the body weight before injection.

Adult↗