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Biomedical subjects

M Kikuchi

Publications and source records attributed to M Kikuchi.

At least 1,135 records · Page 63Linked to original sources
PubMed1977

Necrotizing lymphadenitis: possible acute toxoplasmic infection.

A rapid rise in serum hemagglutination antibody (HA) titers for toxoplasma was detected about 2 months after the onset of cervical lymphadenopathy in two patients with necrotizing lymphadenitis. These lymph nodes proliferation of histiocytes with nuclear debris, phagocytized macrophages and necrotic areas, and the association suggested that necrotizing lymphadenitis might be caused by acute toxoplasmic infection.

Adult↗
PubMed1977

Analysis of the circuitry responsible for primary afferent depolarization in the trigeminal spinal nucleus caudalis of cats.

Depth analysis was performed on the field potential evoked by stimulation of the infraorbital nerve in the trigeminal spinal nucleus caudalis and the subjacent lateral reticular formation of cats. It was shown by dye marking of the recording positions that each subnucleus of the nucleus caudalis (subnucleus marginalis, gelatinosus and magnocellularis) and the reticular formation could be differentiated from one another by the characteristics of the peripherally evoked field potentials. Responses of neurons were extracellularly recorded in the subnuclei gelatinosus and magnocellularis of the nucleus caudalis and in the reticular formation to stimulation of the trigeminal sensory branches (the frontal, infraorbital and lingual nerves), the nucleus ventralis posteromedialis of the thalamus and the cerebral cortex. The properties of the neurons were studied in relation to their thresholds, latencies, receptive fields (sensory branches effective for spike generation) and frequency-following capacities. These responses were then compared with properties of the PAD induced in the fibers terminating in the nucleus caudalis by similar peripheral and central stimulation. It was found that the neurons in the subnucleus magnocellularis were the most likely candidates for the interneurons mediating the peripherally evoked disynaptic PAD in the trigeminal nerve fibers terminating in the nucleus caudalis.

Afferent Pathways↗
PubMed1977

Bronchial carcinoid accompanied by thyroid adenomas and adrenal adenomas.

A large tumor massively occupying the left pleural cavity had the findings of both typical carcinoid and onococytoma which were thought to be of bronchogenic origin. The ultrastructural observation of the tumor revealed a mixture of rod-shaped granules in addition to usual round neurosecretory ones. In the nuclei of dark cells of the oncocytoma, a latticed or hatched structure was detected. Besides two adenomas and hyperplastic foci of large acidophilic cells in the thyroid, a black adenoma and cortical adenoma in the adrenal gland, were detected. Moreover, there was an ectopic adrenal gland in the retroperitoneum. Briefly it was suggested that the bronchial carcinoid presented may be related to multiple endocrine adenomatosis.

Adenoma↗
PubMed1977

Somatostatin- and epinephrine-induced modifications of 45Ca++ fluxes and insulin release in rat pancreatic islets maintained in tissue culture.

The effects of somatostatin and epinephrine have been studied with regard to glucose-induced insulin release and (45)Ca(++) uptake by rat pancreatic islets after 2 days in tissue culture and with regard to (45)Ca(++) efflux from islets loaded with the radio-isotope during the 2 days of culture. (45)Ca(++) uptake, measured simultaneously with insulin release, was linear with time for 5 min. (45)Ca(++) efflux and insulin release were also measured simultaneously from perifused islets. Glucose (16.7 mM) markedly stimulated insulin release and (45)Ca(++) uptake. Somatostatin inhibited the stimulation of insulin release by glucose in a concentration-related manner (1-1,000 ng/ml) but was without effect on the glucose-induced stimulation of (45)Ca(++) uptake. Similarly, under perifusion conditions, both phases of insulin release were inhibited by somatostatin while no effect was observed on the pattern of (45)Ca(++) efflux after glucose.Epinephrine, in contrast to somatostatin, caused a concentration-dependent inhibition of the stimulation of both insulin release and (45)Ca(++) uptake by glucose. Both phases of insulin release were inhibited by epinephrine and marked inhibition could be observed with no change in the characteristic glucose-evoked pattern of (45)Ca(++) efflux (e.g., with 10 nM epinephrine). The inhibitory effect of epinephrine on (45)Ca(++) uptake and insulin release appeared to be mediated via an alpha-adrenergic mechanism, since is was abolished in the presence of phentolamine. Somatostatin inhibits insulin release without any detectable effect upon the handling of calcium by the islets. In contrast, inhibition of insulin release by epinephrine is accompanied by a partial inhibition of glucose-induced Ca(++) uptake.

Animals↗
PubMed1977

SP127, a bacterial protein enhancing the activity of macrolide antibiotics against Pseudomonas aeruginosa: isolation and characterization.

Some strains belonging to the genera Bacillus and Pseudomonas were found to produce in the culture broth, a protein which synergistically enhanced the activity of macrolide antibiotics against Pseudomonas aeroginosa. The protein produced by Bacillus brevis ATCC 8185, designated SP127, was isolated in a pure state by means of ammonium sulfate fractionation, CM-cellulose column chromatography and gel filtration on Sephadex G-100. The sedimentation coefficient and the molecular weight of SP127 were 1.8 s and 15,000, respectively according to the analytical ultracentrifuge. The isoelectric point of SP127, obtained by electrofocusing experiments, was over 10.0. Half cystine and histidine were absent in the molecule.

Amino Acids↗
PubMed1977

Mode of action of the protein, SP127, which enhances the activity of macrolide antibiotics against Pseudomonas aeruginosa.

Antibiotics, the activity of which enhanced against Pseudomonas aeruginosa by SP127, were restricted to the basic macrolide antibiotics such as erythromycin, maridomycin and oleandomycin, the neutral macrolide antibiotics such as lankamycin and lankacidin C, vancomycin and enramycin. Synergistic activity of SP127 with the above antibiotics was found against Pseudomonas aeruginosa and several strains of Escherichia coli, but not against Proteus vulgaris and macrolide-resistant Staphylococcus aureus. SP127 had extremely weak proteolytic but no lytic activity. From the isotopic experiments, the action of SP127 was partially attributed to the promotion of antibiotic penetration to cells of Pseudomonas aeruginosa.

Anti-Bacterial Agents↗
PubMed1977

pH and the activity of porphobilinogen synthase in human blood.

For determination of the activity of this enzyme (EC 4.2.1.24) in blood, pH dependency was evaluated by using whole blood and washed erythrocytes. The effects of assay methods, original and final pH, and use of sodium or potassium phosphate buffer were examined. The pattern of pH dependency differed for whole blood and washed erythrocytes, whether blood from normal or lead-exposed subjects was examined. The pH optimum for the activity in erythrocytes from lead-exposed subjects was the same as for the normal persons if the concentration of lead in blood did not exceed 80 microgram/100 g of whole blood. Hemolyzing blood by placing it on solid CO2 decreases the activity.

Erythrocytes↗