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Biomedical subjects

M Kazama

Publications and source records attributed to M Kazama.

At least 109 records · Page 6Linked to original sources
PubMed1980

Japanese minimum requirements for albumin preparations: recent amendments and current problems.

The following amendments have been introduced to the Minimum Requirements for Human Serum Albumin (HSA) and Plasma Protein Fraction (PPF): 1) The upper limit of sodium content was fixed at 3.7 mg/ml; 2) test for chloride content was introduced; 3) sodium and chloride contents were required to be stated on the label; 4) use of zone electrophoresis was introduced in addition to the free-boundary electrophoresis; and 5) albumin content in HSA was lowered from 97% to 96%. Moreover, current problems in quality control which may require further amendments were discussed, namely: 1) limulus amoebocyte lysate test to replace or supplement the pyrogen test in rabbits; 2) attempt of quantitative expression of visual turbidity; 3) establishment of reference A and B blood group substances; 4) gel permeation analysis and scanning at 370-800 nm; and 5) test for prekallikrein activator (PKA).

Blood Proteins↗
PubMed1979

Participation of kallikrein, coagulation/fibrinolysis parameters in the development of glomerulonephritis.

Masugi nephritis was induced in dogs in which platelet count, fibrinogen, antithrombin activity, plasma prekallikrein and immediate plasmin inhibitors were coincidentally decreased immediately after the injection of nephrotoxin serum. It was found that the grade of decrease of urinary kallikrein excretion following these immediate reactions were parallel with the grade of renal damages. By the pretreatment with heparin or the defibrination with snake venom, however, the histological findings of Masugi nephritis showed rather severe damage. Based on the consumption of coagulation factors, kallikrein, kinin and their inhibitors in the development of this nephritis, it was postulated that inauguration of coagulation and activation of kallikrein contributed to the development of glomerulonephritis. The treatment or prevention of this coagulation process with heparin or snake venom, however, gave untoward effects on the pathological process in this experiment.

Animals↗
PubMed1979

A test method for the absence of thrombogenicity in factor IX complex.

Consumption coagulopathy was developed in three cases of liver cirrhosis and a case of haemophilia B upon administration of Factor IX preparations which had been tested by the manufacturers for the absence of active clotting factors according to the Japanese Minimum Requirements for Biological Products. By employing the TGT determination, however, active clotting factor(s) could be detected in some of the preparations used in these cases. Accordingly, it was found necessary to modify the current test method in the Minimum Requirements by introducing calcium ion into the test medium. There was no correlation between the clotting activitiy detected by our modified test method and the Factor IX potency of the product. Addition of heparin did not significantly influence the results in our modified method.

Adult↗
PubMed1977

Formation of soluble fibrinogen-fibrin complex in liquid human plasma; reflexion of coagulation process in storage.

Analysis was performed on a high-molecular fraction with fibrinogen antigenicity present in Liquid Human Plasma. This fraction, although seemed to resemble macromolecular FDP (fibrinogen degradation product) derived from stabilized fibrin, has many features not shared with the latter: 1) This fraction is sensitive to sodium dodecylsulfate (SDS) treatment. 2) Degradation products of this fraction obtained by digestion with plasmin, when eluted from a column of Sephadex G-200, exhibited a single peak of FDP-D antigenicity at the position of D-monomer. 3) In SDS polyacrylamide gel electrophoresis in the presence of dithiothreitol (DDT), no evidence for the presence of gamma-dimer was obtained. Thus, it is unlikely that the high-molecular fraction is formed through interchain cross-linking between fibrinogen molecules. The formation of the high-molecular complex was discussed in relation to coagulation process in stored plasma.

Antigens↗