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Biomedical subjects

M Kaur

Publications and source records attributed to M Kaur.

At least 91 records · Page 5Linked to original sources
PubMed1990

Lytic effect of Vibrio cholerae elastase on gram-positive and -negative bacteria.

Elastase of Vibrio cholerae caused the lysis of freshly grown cells of Gram-negative (Pseudomonas aeruginosa, Proteus vulgaris, Salmonella paratyphi A and Klebsiella pneumoniae) bacteria. Gram-positive (Staphylococcus aureus and S. epidermidis) organisms were resistant to this enzyme. Heat killed and lyophilized Gram-positive and -negative bacteria (except S. aureus and S. epidermidis) showed higher sensitivity to elastase. Both Gram-negative and -positive bacteria were lyzed maximally by elastase at pH 8.0. At this pH, lytic activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.

Bacteriolysis↗
PubMed1990

Effect of Nilzan and albendazole on the absorptive surfaces of Haemonchus contortus (Nematoda)--a histoenzymic study.

Haemonchus contortus, incubated in 10 micrograms/ml and 50 micrograms/ml concentrations of Nilzan and albendazole in Tyrode solution were stained for histoenzymatic demonstration of various phosphatases, oxido-reductases and esterases. The intestine showed major alterations after drug treatments. The alkaline phosphatases (AkPase), adenosine triphosphatase (ATPase), glucose-6-phosphatase, succinic dehydrogenase (SDH), glutamate dehydrogenase (GDH), reduced nicotinamide adenine dinucleotide phosphate diaphorase and reduced nicotinamide adenine dinucleotide diaphorase showed a decreased activity in intestine after Nilzan treatment, whereas lactic dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PD) and monoamine oxidase resisted increased reaction. The albendazole treatment resulted in altered distribution pattern of the AkPase, ATPase, SDH, and GDH; while LDH, G-6-PD, and non-specific esterases exhibited slightly enhanced activity in the epithelium. The functional significance of these changes has been fully discussed.

Absorption↗
PubMed1989

Lytic effect of Pseudomonas aeruginosa elastase on gram-positive and -negative bacteria.

Elastase of Pseudomonas aeruginosa P3 caused lysis of freshly grown cells of Gram-positive (Staphylococcus aureus and S. epidermidis) and Gram-negative (Proteus vulgaris and Salmonella typhi) bacteria. Heat killed and lyophilized Gram-negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, the activity of elastase was maximal in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and Cacodylate buffers.

Bacteriolysis↗
PubMed1989

Production and partial characterization of elastase of Vibrio cholerae Inaba.

Conditions are described for the production of extracellular elastase by Vibrio cholerae Inaba 2/57. The yield of the enzyme was maximum in shake cultures grown in alkaline peptone water at 37 degrees C and was stable in culture supernatants. The enzyme, partially purified by ammonium sulphate precipitation and Sephadex G-100 gel filtration showed a molecular weight of 30,000 and an activity between pH 5.0-8.0 with an optimum of 8.0 in Tris-maleate buffer. The elastinolytic activity was maximal in glycine-NaOH buffer and minimal in phosphate buffer. The enzyme activity was adversely affected by temperature greater than or equal to 40 degrees C.

Chromatography, Gel↗
PubMed1989

Effect of sodium selenite on antioxidative enzymes of banana fruitfly.

The median and maximum life spans of Zaprionus paravittiger are 43 and 79 days, respectively, for males and 52 and 91 days for females at 24 +/- 2 degrees C. Sodium selenite (SS), an antioxidant, feeding prolongs the median as well as maximum life span of both the sexes. Antioxidative enzymes, catalase and peroxidase showed greater activity during the developmental stages. Females, at most of the age intervals, exhibited greater enzymatic activities as compared to their age-matched males. Both the sexes showed a statistically significant increase in the baseline activity of these enzymes as consequent upon SS feeding. It is suggested that SS acts by strengthening the antioxidative enzymes, namely catalase and peroxidase.

Aging↗
PubMed1988

An antioxidant induced alterations in peroxidase activity in ageing Zaprionus paravittiger (Diptera).

Sodium hypophosphite (1 x 10(3) microM) supplementation in the diet of Zaprionus paravittiger resulted in adaptive responses in the quantitative as well as qualitative activity of peroxidase. The changes in peroxidase were in compliance with the earlier reported sodium hypophosphite (SHP)-induced prolonged life span of flies (Wadhwa, R., Arch. Gerontol. Geriatr., 6 (1987) 141-147) and support the concept that antioxidative enzymes may serve as important longevity determinants.

Animals↗
PubMed1988

Bacteriology of the cervix in cases of infertility: effect on human and animal spermatozoa and role of elastase.

Microorganisms such as Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans isolated from cervices of infertile human females inhibited motility and agglutinated human, cow bull, buffalo bull, and rat spermatozoa in vitro. Fifty percent of the infertile females studied carried elastase-positive microorganisms. Cell-free culture supernatants of 72-hr-old elastase-positive cultures were spermicidal within 60 min of contact with sperm, while elastase-negative cultures were spermicidal in 4-6 hr. Cultures of all the cervical isolates were spermicidal and agglutinated human, cow bull, buffalo bull, and rat spermatozoa, and these activities increased with age of the culture. Human sperm showed only tail-to-tail agglutination, while cow bull, buffalo bull, and rat spermatozoa showed mainly head-to-head agglutination. Spermicidal activity was also attributable to elastase, which was present more in 72-hr-old cultures than in 24-hr-old cultures.

Agglutination↗
PubMed1988

Production and partial characterization of elastase of Bacillus subtilis isolated from the cervices of human females.

Conditions are described for the production of extracellular elastase by Bacillus subtilis. The yield of enzyme was maximum in shake-cultures grown in Syncase medium at 37 degrees C and was stable in culture supernatants. The enzyme, purified by ammonium sulphate precipitation and Sephadex G-75 gel filtration, showed a molecular weight of 25,000 and activity between pH 6.0 and 9.5, with an optimum of 9.0 in Tris-maleate buffer. Elastinolytic activity was maximum in glycine-NaOH buffer and minimum in phosphate buffer. Enzyme activity was adversely affected by temperature greater than or equal to 40 degrees C.

Bacillus subtilis↗
PubMed1986

Isoenzyme studies of Giardia lamblia isolated from symptomatic cases.

Strains of Giardia lamblia were isolated from symptomatic cases of giardiasis and axenized in the laboratory. Electrophoretic mobility patterns of four enzymes, viz., EC 5.3.1.9 glucose phosphate isomerase (GPI); EC 1.1.1,4.0.L-malate; NADP+ Oxidoreductase (Oxaloacetate decarboxylating) (ME); EC 2.7.5.1 phosphoglucomutase (PGM); and EC 2.7.1.1 hexokinase (HK) of the lysates prepared from these isolates were studied using starch-gel. Based on differences in mobility patterns of PGM and HK, the four strains studied could be grouped into three different isoenzyme types (Zymodemes). ME mobility was identical in all the four strains. Some relative difference was seen in the mobility of GPI, though the pattern of mobility was similar in all the strains.

Adolescent↗
PubMed1986

Bacteriology of cervix in cases of infertility: effect on human sperm.

Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis isolated from cervices of infertile females possessed spermicidal activity. They also agglutinated the human spermatozoa in vitro and showed tail-to-tail agglutination. Cell-free supernatant of these organisms was found to be spermicidal but did not agglutinate spermatozoa in vitro. Spermicidal activity was increased with increase in age of the culture.

Bacterial Physiological Phenomena↗