[Neo-expression of cytokeratin 7 in chronic atrophic gastritis with pernicious anemia].
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Adolescent↗
Biomedical subjects
M Kasper
Publications and source records attributed to M Kasper.
Age-dependent pattern of intermediate filament protein expression in the human pineal gland.
Intermediate filament distribution was studied in children and adult human pineal glands by using immunocytochemistry and immunoblotting. The demonstration of cytokeratin positive cell clusters in the infant pineal gland may be related to ependymal cells arising from the subcommissural organ. The SCO ependymal cells and the ependymal cells in the pineal parenchyma express the neuro-ectoderm specific individual cytokeratins no. 8 and 18. Cytokeratin positive ependymocytes additionally exhibited vimentin and were serotonin negative as revealed by double immunofluorescence labelling. By contrast, in adult pineal glands cytokeratin positive cells were not clustered. An interesting but unexplained increase of neurofilament 68 KD positive nerve fibres was detectable.
Adult↗
[Mucourothelial cancer of the urinary bladder and ureter].
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Adenocarcinoma, Mucinous↗
[Immunohistochemistry of cytokeratin expression in human blood vessel endothelia with special reference to synovial connective tissue].
41 tissue samples derived from the synovia, epidermis, and other organs were studied immunohistochemically using monoclonal antibodies against intermediate filaments. We observed a coexpression of cytokeratin and vimentin in endothelia of small blood vessels preferably enriched in locations with enhanced fluid transport and processes of ultrafiltration via endothelial cells (synovial tissue, ganglion). The presence of cytokeratin 18 in endothelial cells has been confirmed by gel-electrophoresis with immunoblotting. In many cases, the coexpression of cytokeratin and vimentin reveals a correlation to the secretion of simply and epithelially differentiated cells as could be demonstrated for other tissue structures in previous studies. The increased expression of cytokeratins in endothelia of ganglion walls is discussed in relation to synovia-like misfunction of the joint connective tissue.
Antibodies, Monoclonal↗
Patterns of expression of tumor markers on non-transformed human mammary epithelial cells cultured in vitro.
We have examined the expression of 7 well defined tumor markers/tumor associated antigens (H type 2, X, Y, sialyl-Lea, CEA, MAM-6, and Tn) and a tumor associated antigen defined by a new own monoclonal antibody on non-transformed human epithelial cell lines derived from reduction mammoplasties by means of immunocytochemistry with monoclonal antibodies. Two cell types are discernible: slowly or non-proliferating, lumenal derived (I), and proliferating, stem cell-like, basal cell-derived cells (II). Five out of the 8 tumor markers were expressed on type I cells, and all 8 on type II cells. The number of positive cells varied considerably from a few to 100 per cent depending on the individual markers. The observed patterns proved to be characteristic and reproducible; they appear to reflect the developmental stage of the cells cultured in vitro rather than a direct influence of the culture conditions.
Biomarkers, Tumor↗
Immunohistochemical investigation of different cytokeratins and vimentin in the human epididymis from the fetal period up to adulthood.
The anatomical distribution of cytokeratins and vimentin was investigated by means of immunohistochemistry in the human epididymis. Epithelial cells of the ductuli efferentes and the corpus epididymidis were positive for cytokeratins and vimentin. The expression of epithelial vimentin decreased toward the cauda epididymidis, whereas cytokeratins remained unchanged. The epithelium of the ductus deferens was negative when antibodies against vimentin were used. With monoclonal antibodies to individual cytokeratins, the presence of cytokeratins 7, 8, 18, and 19 was demonstrated histochemically throughout the epithelium of the epididymis. Monoclonal antibodies specific for cytokeratin 17 allowed immunohistochemical differentiation between the ductuli efferentes and the ductus epididymidis.
Aging↗
Cell type heterogeneity of intermediate filament expression in epithelia of the human pituitary gland.
In the present study we have localized immunohistochemically the intermediate filament proteins of the human pituitary gland (adenohypophysis, pars intermedia and pars tuberalis) by an indirect immunoperoxidase technique or by double immunofluorescence methods and analysed the individual cytokeratin polypeptides using two-dimensional gel electrophoresis. We found that the expression of cytokeratins in different epithelial cells of the human anterior pituitary gland was heterogeneous. Whereas the endocrine cells only expressed cytokeratins 8 and 18, the folliculo-stellate cells exhibited a reactivity for cytokeratins 7, 8, 18 and 19 as well as for GFAP and vimentin. The squamous epithelial cells of the pars tuberalis and the Ratke's cysts showed a more complex cytokeratin pattern of both squamous and simple type. Whereas in may cystic epithelial cells including the "pseudo-follicles" a triple expression of cytokeratin, vimentin and GFAP could be observed, only some basal cells of squamous epithelial nests coexpressed cytokeratin and vimentin. The differences in the intermediate filament protein distribution are discussed in the light of embryological relationships of the different parts of the human pituitary gland.
Desmin↗
Primary duodenal carcinoma arising in a non-vaterian tubulo-villous adenoma. A case report with immunocytochemical analysis and review of the literature.
Primary duodenal carcinoma and duodenal adenoma are rare tumours. Duodenal carcinoma makes up about 0.3% of all malignant tumours of the gastrointestinal tract (Alwmark et al. 1980; Spira et al. 1977). The present paper describes a duodenal carcinoma arising in a mixed tubulo-villous non-Vaterian adenoma in a 68 year old male. Immunocytochemical analysis revealed evidence of neuroendocrine differentiation in both adenoma and carcinoma. In a review of the literature a correlation between the size of adenoma and the probability of concomitant carcinoma is demonstrated. Duodenal adenoma measuring more than 4 cm in diameter should be considered potentially malignant.
Adenocarcinoma↗
Localization of proinsulin and insulin in human insulinoma: preliminary immunohistochemical results.
We have carried out an immunohistochemical investigation of 15 human insulinomas applying monoclonal antibodies specifically recognizing proinsulin and insulin. Our results demonstrate that the epitopes unique to proinsulin and insulin can be detected with the respective monoclonal antibodies using the protein A-gold technique after routine formaldehyde fixation and paraffin embedding of the tissues. The immunostaining pattern for proinsulin and insulin in the insulinomas was different from the observed in B cells of pancreatic islets present in the adjacent normal pancreas. Furthermore, the pattern of immunostaining was found to vary from tumor to tumor. These findings strongly suggest the possibility of a disturbed proinsulin to insulin conversion in human insulinomas.
Adenoma, Islet Cell↗
EDTA-polyvinylpyrrolidone as a preservation medium for immunohistochemistry.
In a solution on 7% EDTA and 5% polyvinylpyrrolidone in 0.1 mol/l. Tris buffer, pH = 6.95, tissues samples can be stored at room temperature for 1 week without detectable loss of structural integrity or antigenicity (as demonstrated in immunohistochemistry with monoclonal antibodies to intermediate filament proteins).
Biomarkers, Tumor↗
Patterns of cytokeratin/vimentin coexpression in the guinea pig.
The distribution of cytokeratin and vimentin in guinea pig tissues as seen by immunohistochemistry using monoclonal antibodies is described and a similar distribution pattern of coexpression of cytokeratin and vimentin in various cell types as compared to human tissues were found. The possible explanations for the unusual coexpression of both types of intermediate filaments in normal cells are discussed.
Animals↗
Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin.
We applied immunohistochemical techniques and gel electrophoresis to examine the distribution of intermediate filaments in human fetal oral epithelium and the epithelia of the human enamel organ. Both methods demonstrated that human enamel epithelia contain cytokeratins 5, 14, and 17, which are typical of the basal cells of stratified epithelia, as well as smaller quantities of cytokeratins 7, 8, 19, and in trace amounts 18, which are characteristic components of simple epithelial cells. In the external enamel epithelium and stellate-reticulum cells, most of these components appeared to be simultaneously expressed. In contrast, the parental oral epithelium was negative for cytokeratin 7, thus indicating possible "neoexpression" during the course of tooth formation. Immunohistochemical procedures using various monoclonal antibodies against vimentin revealed the transient coexpression of vimentin and cytokeratins in the external enamel epithelium and in stellate-reticulum cells during enamel development. The significance of the coexpression of cytokeratins and vimentin is discussed in relation to previous findings obtained in other normal tissues and in the light of the functional processes characteristic of these epithelia.
Antibodies, Monoclonal↗
Age-related changes in the expression of cytokeratin and vimentin in human choroid plexus.
Using immunohistochemical methods the expression of intermediate filaments in fetal and adult human choroid plexus epithelium was investigated. The results indicate that the full expression of cytokeratin and vimentin was seen at the 22nd gestational week corresponding to the onset of liquor production. In neural tube and crest cells of human embryos no cytokeratins were detectable, whereas vimentin could be demonstrated in early stages.
Aging↗
[Retrospective immunohistologic search for metastases using monoclonal anti-cytokeratin antibodies in lymph nodes of patients with stage Ib-IIb cervix cancer dying within 5 years].
With immunohistological methods using monoclonal antibodies more metastases were detected in carcinomas today. We examined 10 women, where the carcinoma of the cervix was removed today in healthy and where the lymph nodes were free of metastases. With use of the monoclonal cytokeratin-antibody lu-5 it was not possible to improve the diagnostic. In a histomorphological differentiation of grade 2 or 3 a radiation should be considered.
Adult↗
Coexpression of cytokeratin and vimentin in Rathke's cysts of the human pituitary gland.
Immunohistochemistry with monoclonal and polyclonal antibodies revealed the presence of cytokeratins in epithelial cells of Rathke's cysts in the pars intermedia of the human pituitary gland. With monoclonal antibodies specific for individual cytokeratins, the expression of CK 18, CK 8, CK 7, and CK 19 could be shown in these cells. Within the hypophysis, CK 19 and CK 7 were restricted to Rathke's cysts and a few epithelial cell clusters in the pars tuberalis, whereas other cytokeratins were also present in endocrine cells of the pars distalis. Furthermore, vimentin and, focally, glial fibrillary acidic protein (GFAP) were detected in the cystic epithelia. By double labelling, coexpression of cytokeratin and vimentin, GFAP and cytokeratin, and GFAP and vimentin could be demonstrated. Compiled data of all known cases of coexpression of cytokeratin and vimentin in normal cells reveal physiological correlations and suggest a functional significance of this rare type of coexpression of intermediate filament proteins.
Aged↗
Patterns of cytokeratin and vimentin expression in the human eye.
We studied the expression of the various cytokeratin (CK) polypeptides and vimentin in tissues of the human eye by applying immunocytochemical procedures using a panel of monoclonal antibodies as well as by performing biochemical analyses of microdissected tissues. Adult corneal epithelium was found to contain significant amounts of the cornea-specific CKs nos. 3 and 12 as well as CK no. 5, and several additional minor CK components. Among these last CKs, no. 19 was found to exhibit an irregular mosaic-like staining pattern in the peripheral zone of the corneal epithelium, while having a predominantly basal distribution in the limbal epithelium. Both the fetal corneal epithelium and the conjunctival epithelium were uniformly positive for CK no. 19. In the ciliary epithelium, co-expression of CKs nos. 8 and 18 and vimentin was detected, whereas in the retinal pigment epithelium, CKs nos. 8 and 18 were dominant. The present data illustrate the remarkable diversity and complexity of CK-polypeptide expression in the human eye, whose significance with respect to histogenetic and functional aspects is, as yet, only partially clear. The unusual distribution of CK no. 19 in different zones of the corneal epithelium may be related to the specific topography of corneal stem cells. The occurrence of the expression of simple-epithelium CKs in the ciliary and pigment epithelium demonstrates that, despite their neuroectodermal derivation, these are true epithelia.
Ciliary Body↗
Coexpression of cytokeratins and vimentin in hyaluronic acid-rich tissues.
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Antibodies, Monoclonal↗
A new monoclonal antibody (A46-B/B10) highly specific for the blood group H type 2 epitope: generation, epitope analysis, serological and histological evaluation.
A monoclonal antibody recognizing the blood group H type 2 antigen has been obtained from a BALB/c mouse immunized with MCF-7 (human mammary carcinoma) cells. The specificity of this antibody (A46-B/B10, IgM, kappa) has been identified by haemagglutination tests, immunohistochemistry, binding inhibition studies, and absorption experiments performed with synthetic oligosaccharides. The antibody is virtually nonreactive with H type 1 antigen or with closely related type 2 structures (e.g., Y antigen). A46-B/B10 strongly agglutinates human erythrocytes according to the amount of H substance expressed and can, therefore, easily discriminate between blood groups A1 and A2 as well as A1B and A2B (A1 and A1B are not or only weakly agglutinated). In immunohistochemistry, this antibody seems to provide a highly specific reagent for a restricted number of carcinomas and epithelial lineages in tissue sections and in vitro.
ABO Blood-Group System↗