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M Kasper

Publications and source records attributed to M Kasper.

At least 181 records · Page 10Linked to original sources
PubMed1988

Expression of cytokeratin and vimentin in nucleus pulposus cells.

With immunohistochemistry using monoclonal antibodies against intermediate filament proteins, nucleus pulposus cells were found to express cytokeratin(s) simultaneously with vimentin in fetal life and childhood. This finding adds to the series of human tissues showing coexpression of cytokeratins and vimentin. Surprisingly remnants of such cells were also found in the nucleus pulposus of adults, and a possible relationship of such cells to chordoma formation is discussed.

Antibodies, Monoclonal↗
PubMed1988

[Immunohistochemical studies with monoclonal antibodies in human mammary carcinoma tissues].

In an immunohistochemical investigation 27 mammary carcinoma tissues were tested with a panel of monoclonal antibodies against cytokeratins, blood group-related and carcinoembryonic antigens. The monoclonal antibody A45-B/B3 directed against a broad range of cytokeratins reacted with all carcinoma tissues tested and was also useful for the detection of metastasis in axillary Lymph nodes of mammary carcinoma patients. The expression of blood group-related substances in tumor cells was different, but without relation to the donor's red blood cell phenotype. In our study the detection of CEA was restricted to malignant breast tumors and was present in two thirds of them.

Antibodies, Monoclonal↗
PubMed1988

[Detection of cytokeratin in cells of the arachnoid and in meningiomas].

Only few investigations have so far become known in the context of cytokeratin expression in cells of the arachnoid and in meningioma. Immunohistochemical studies, using three monoclonal anti-cytokeratin antibodies, were conducted into six arachnoids and 43 meningiomas. Consideration was given to all histological types of meningioma but the papillary type. Three arachnoids and nine meningiomas responded positively to cytokeratin. The highest ratio of positive meningiomas, that is four in ten, was recorded from unfixed cryostat sections, using the monoclonal antibody A45-B/B3.

Arachnoid↗
PubMed1988

[A monoclonal antibody against peripheral human blood monocytes (RoMo-1)].

The monoclonal IgG2a-antibody RoMo-1 binds to peripheral human blood monocytes but does not react with other blood cells, tissue macrophages, HL-60, K 562 or U 937. It seems possible, that the antibody defines an antigen expressed on the premonocytic and monocyte stages of cells from the mononuclear phagocytic system only. The antibody is cytotoxic.

Antibodies, Monoclonal↗
PubMed1988

2-bromo-1-naphthol--a novel chromogen for the immunoperoxidase technique in immunohistochemistry.

2-bromo-1-naphthol is a new chromogen for the immunoperoxidase reaction in immunodot assays on nitrocellulose filters and on Western blots and could now also be employed in immunohistochemistry at the light microscopic level. It displays a blue-violet reaction product which is insoluble in aqueous solutions and almost insensitive to light. By means of this substrate for the peroxidase reaction in conjunction with 3,3'-diaminobenzidine it is also possible to perform double labellings.

3,3'-Diaminobenzidine↗
PubMed1988

[Neuropathology of hypertensive encephalopathy].

An account is given of hypertensive encephalopathy on the basis of neurohistological studies into 54 brains, with additional reference being made to autopsies. Vasculopathy of peripheral arteries and arterioles along with thickening of vascular walls, change of media, and lumen stenosis were primarily recorded by means of optical light and electron microscopy. A difference of more than seven per cent was found to exist between wall thicknesses of arterioles in hypertensive brains, on the one hand, and those in normotensive brains, on the other. Vessels with thickened walls were not rigid but were rather capable of dilation just as under normotensive conditions. Their inner diameters, however, were below normal. Vascular wall fibrosis, to which little attention had been given in the past, was repeatedly recordable from the venous and capillary systems. Impairment of the endothelial barrier function was quite often recorded, with lipid deposits being incorporated in peripheral arterioles and capillaries. No relevance to pathogenesis can be reliably attributed at present to detection of IgG in the arterial system. Microinfarction, micro-haemorrhage as well as disseminated damage to nerve cells are recordable from roughly one third of all hypertensive brains and are primarily located in brain stem ganglia. Demyelinisation and cerebral oedema are quite infrequent. High-protein cerebral oedema was found in some rare cases. Hypertensive encephalopathy should be considered as a nosological entity in which morphological and clinical phenomena are not always correlated to each other.

Arterioles↗
PubMed1987

Focal ependymal differentiation in the choroid plexus of human newborn brain confirmed by immunohistochemistry with monoclonal antibodies.

Ependymal proliferation ["Ependymzotten", G. Becker, Beitr Pathol Anat Allg Pathol 103:457-477 (1939)], found in choroid plexus of newborns, can be made easily visible by immunostaining with antiglial fibrillary acidic protein (GFAP) antibodies. The expression of both GFAP and cytokeratin is an unusual feature of these structures.

Cell Differentiation↗
PubMed1987

Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.

Three new monoclonal anti-cytokeratin antibodies (mabs) potentially useful in cancer research and clinical diagnosis have been evaluated in immuno-histochemistry on cryostat sections of a broad variety of normal human tissues. A45-B/B3 reacts with all cells containing cytokeratins (epithelia and mesothelia). This mab positively identifies epithelial cells of any kind, and it may serve in differentiating carcinomas from tumours of mesenchymal origin. A53-B/A2 recognizes an individual cytokeratin, No. 19, and stains preferably mesothelia, urothelium, and bile duct epithelium. This antibody is suited to discriminate between different epithelial lineages. A51-B/H4 reacts with a subgroup of cytokeratins (probably including Nos. 14, 8 and/or 18). It is positive with most epithelia but negative with keratanized stratified epithelium. This antibody shows an interesting, but up to now unexplained, cross-reactivity with nuclei of certain nonepithelial cells. All three mabs also react with epithelial cells from at least three animal species.

Animals↗
PubMed1987

The expression of subunits of human chorionic gonadotropin (hCG) by nontrophoblastic, nonendocrine, and endocrine tumors.

The value of the subunits of human chorionic gonadotropin (hCG) as tumor markers is controversial. The production of hCG-alpha and hCG-beta by 214 nontrophoblastic exocrine and by 416 endocrine tumors was analyzed by using immunocytochemical technics. hCG-alpha immunoreactivity was found in 131 of 416 endocrine and in 8 of 214 nonendocrine tumors. hCG-beta could not be visualized specifically with the use of two polyclonal antisera and one monoclonal antibody. The authors conclude that (1) hCG-alpha is neither a tissue nor a tumor-specific marker; (2) hCG-alpha is produced by a variable proportion (21-55%) of endocrine tumors arising in various organs but not by ileal carcinoids, paragangliomas, pheochromocytomas, nor Merkel cell tumors; (3) hCG-alpha is a marker of malignancy only in pancreatic endocrine tumors; and (4) hCG-beta is rarely, if ever, produced by tumors.

Carcinoid Tumor↗
PubMed1987

Immunocytochemistry of pituitary tumors.

Pituitary tumors from 376 patients were investigated, using immunocytochemical techniques at the light and electron microscopic level, and autoradiography combined with immunocytochemistry for localizing somatostatin (SRIH) receptors. Prolactinomas, growth hormone-secreting adenomas causing acromegaly, and hormonally inactive adenomas were most frequently observed (153, 86, and 90 tumors, respectively). Among the latter, we could distinguish "alpha-only adenomas," many of which were oncocytomas. At the light and electron microscopic levels, cells containing (and presumably producing) simultaneously both prolactin and growth hormone, and cells containing exclusively either prolactin or growth hormone, could be demonstrated. In addition, a highly variable number and distribution of SRIH receptors could be shown in tumors secreting prolactin, growth hormone, and in tumors not associated with symptoms caused by inappropriate hormone secretion. The systematic combination of clinical, radiological, and biological techniques has currently brought great progress in the behavior and therapeutic concepts of pituitary lesions, and promises new achievements in the near future.

Adenoma↗
PubMed1987

[Comparative immunohistochemical and lectin histochemical studies of ependymal cells and the epithelium of the choroid plexus].

In a previous investigation we reported on the distribution of intermediate filaments in normal epithelium of choroid plexus and ependyma (Kasper et al. 1986). The present paper describes the results obtained with an enlarged panel of monoclonal antibodies against intermediate filaments and additionally with lectins. Ependymal cells contain GFAP and vimentin filaments, whereas epithelial plexus cells express cytokeratin, vimentin and neurofilament 200 KD. Using Concanavalin A, we found a strong cytoplasmatic staining of plexus epithelia and a reduced or failed reaction in ependymal cells. The differences in both cell types may find an explanation in their function or are determined during the ontogenic development.

Animals↗
PubMed1987

[Light microscopic studies of concanavalin A binding in the choroid plexus epithelium and ependymal cells of the cerebral ventricle and human tumors derived from those tissues].

Histochemical investigations of human choroid plexus with lectins revealed strong cytoplasmic binding of concanavalin A to plexus epithelium, whereas ependymal cells were unstained or only weakly reactive. A similar but less clear-cut staining pattern by concanavalin A was observed with plexus papillomas and ependymomas. A clear discrimination however, between the two tumor types using this method is not possible.

Cerebral Ventricle Neoplasms↗
PubMed1986

Coexistence of cytokeratin, vimentin and neurofilament protein in human choroid plexus. An immunohistochemical study of intermediate filaments in neuroepithelial tissues.

The expression of intermediate filament proteins in human brain ependyma and choroid plexus epithelium has been studied by immunohistochemistry using a panel of monoclonal antibodies directed against all five classes of intermediate filaments. Ependymal cells express GFAP and vimentin filaments, whereas plexus epithelium simultaneously contains neurofilaments, cytokeratins and vimentin, a phenomenon not previously observed in normal cells in vivo. By means of specific antibodies we were able to establish that cytokeratins 8 and 18 but not 19 are present in plexus epithelium.

Aged↗
PubMed1986

"Brunneroma": hamartoma or tumor?

Clinically relevant "tumors" of duodenal glands (Brunner's glands) are uncommon. We present a patient suffering from an exceptionally large tumor of the pars descendens of the duodenum. The tumor remained undetected at endoscopy and is of interest because of its localization, size and its presumable production of urogastrone (human epidermal growth factor). "Brunneromas" are probably hamartomas rather than true neoplasias.

Adult↗