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Biomedical subjects

M Kaplan

Publications and source records attributed to M Kaplan.

At least 217 records · Page 12Linked to original sources
PubMed1990

Use of monoclonal antibody 17.13 in the diagnosis of human oral squamous cell carcinoma.

We assessed the ability of monoclonal antibody (MAb) 17.13 to react with human oral squamous cell carcinoma (OSCC) and other oral tissues. MAb 17.13 was reacted with acetone-fixed, serial frozen sections of 21 OSCC, 7 fibromas, 2 squamous papillomas, 1 melanosis and 3 normal oral mucosa, by means of an avidin-biotin-immunoperoxidase assay. Twenty-one of 21 tumors (100% sensitivity) reacted strongly with MAb 17.13, with a homogeneous staining pattern. Individual tumor cells could be clearly seen, improving the detection of microinvasion or borderline lesions. The epithelium of benign lesions and normal oral mucosa showed staining of the basal cells only. We conclude MAb 17.13 could be used as an accurate diagnostic tool for OSCC.

Antibodies, Monoclonal↗
PubMed1990

The dual effect of epidermal growth factor upon human chorionic gonadotropin secretion by the first trimester placenta in vitro.

Epidermal growth factor (EGF) is believed to play an important role in the regulation of placental function. We have examined the effect of EGF upon first trimester (7-10 gestational weeks) placental hCG secretion and cellular differentiation using both static (explants and isolated cells) and kinetic (superfusion of explants) culture methods. In superfused explants, short (1-4 min) pulses of EGF increased both the rate and amplitude of spontaneous pulsatility of hCG. The frequency increased from 3/h to 5/h, and the amplitude increased compared to the control channels as calculated by the area under the curve. This effect was dose dependent and the concentration of 50 ng/ml, which was the lowest dose tested, was the most effective. In explants cultured for 24 h, EGF caused a 2-fold increase in hCG secretion, compared to control, P less than 0.05. In two different dispersed trophoblastic cell cultures, EGF added daily for the first week caused a 180% increase in hCG secretion, P less than 0.05. However, according to morphological criteria, i.e. light microscopy and vital staining, no significant effect upon the rate of differentiation to syncytiotrophoblast was observed in long-term cultures of one of these preparations. In conclusion, EGF plays a dual trophic role in stimulating hCG secretion in the first trimester. However, this effect is not dependent on cellular differentiation.

Cell Differentiation↗
PubMed1990

Geriatric and team training for podiatric residents.

The authors discuss the Interdisciplinary Team Training in Geriatrics program developed by the Department of Veterans Affairs. At the James A. Haley Veterans Hospital, this program provides funding to podiatric residents, enabling them to work with practitioners from other health care disciplines as a team that addresses all of the varied health needs of the elderly patient.

Florida↗
PubMed1990

Effects of histamine on alpha adrenergic receptor expression on the lymphocytes of normal and asthmatic subjects.

Low levels of [3H]-dihydroergocryptine (DHE) (alpha adrenergic receptor) binding to peripheral blood mononuclear leukocytes from asthmatic subjects were significantly increased following incubation with histamine. In contrast, [3H]-DHE binding was undetectable both before and after histamine incubation using cells from nonasthmatic subjects. The change in binding showed a biphasic dose dependence on histamine and was not associated with changes in DHA (beta-2 receptor) or yohimbine (alpha-2 receptor) binding. The induction of alpha adrenergic receptors by the ubiquitous inflammatory mediator histamine may help explain the alpha adrenergic hyperresponsiveness seen in asthmatic patients.

Asthma↗
PubMed1989

Extramural cross-validation of the Mayo primary biliary cirrhosis survival model establishes its generalizability.

The generalizability of the Mayo model for predicting survival in individual primary biliary cirrhosis patients without liver transplantation was tested and confirmed. The model was applied to a data base of patients from the New England Medical Center Hospitals (n = 141) and the Scott and White Clinic (n = 35) and found to predict their survival accurately. It was also shown to be accurate for Mayo primary biliary cirrhosis patients with very advanced disease (n = 30), those with less than a 33% chance of surviving 12 months. The analyses confirmed that the addition of histologic stage did not significantly improve the predictive power of the model (p greater than 0.10). We suggest that the Mayo model is a practical tool for clinical management and decision making.

Academic Medical Centers↗
PubMed1989

Human retroviruses: a common virology.

There are five known human retroviruses: human T-lymphotropic virus-I (HTLV-I), HTLV-II, HTLV-V, human immunodeficiency virus-1 (HIV-1), and HIV-2. These are related to animal lentiviruses. The simian retroviruses, simian T-lymphotropic virus-I (STLV-I) and STLV-III are related closely to HTLV-I and HIV-2 respectively. HTLV-I and HTLV-II and, possibly, HTLV-V are transforming agents that immortalize the CD4 cell. In contrast, HIV-1 and HIV-2 cause this cell to lyse, resulting in immunodeficiency (ID). HIV-1 and HIV-2 cause severe ID resulting in acquired immunodeficiency syndrome (AIDS). In HTLV-I and HTLV-II, ID is less severe and rarely progressive. Both of these retroviruses induce proliferation of CD4 cells. In HTLV-I, this results in acute T cell leukemia and mycosis fungoides (MF) with hypercalcemia. HTLV-V produces a less severe form of MF without hypercalcemia. Associated lymphomas (AL) occur with HTLV-I. HIV-1 and HIV-2 produce AL as well as Kaposi's sarcoma. Both also cause subcortical dementia because they are neurotropic. All human retroviruses appear to be transmitted sexually and by blood. Transfusional AIDS may be almost entirely eliminated by serologic testing of the blood supply, and transfusional lymphoma can be almost entirely eliminated by universal testing for HTLV-I.

HIV-1↗
PubMed1989

Screening for hypoglycemia with plasma in neonatal blood of high hematocrit value.

A model utilizing human umbilical cord blood was used to screen for hypoglycemia in a simulated neonatal situation. The aims of the study were to assess the effect of increasing Hct concentration on Dextrostix readings, and to determine whether plasma samples, in contrast to conventional whole blood samples, were acceptable for Dextrostix determinations in selected cases. Dextrostix readings were determined on 65 whole blood samples of varying Hct, and on plasma specimens, 48 of which were paired with whole blood samples. The results were compared with plasma true glucose values. Plasma Dextrostix readings correlated well with true glucose values throughout the entire Hct range (r = .94, slope = 1.16). Whole blood Dextrostix readings, on the other hand, were Hct-dependent and, with increasing Hct values, became falsely low. As a result, a Hct values greater than 70%, whole blood Dextrostix readings were less than 50% of the true glucose value (r = .94, slope = 0.45). Plasma Dextrostix determinations may offer an accurate means of screening for neonatal hypoglycemia in asymptomatic infants with high Hct, pending laboratory glucose results.

Blood Glucose↗
PubMed1989

Chronic neonatal coxsackie myocarditis.

A baby girl, with a birth weight of 2540 g, developed myocarditis with gross, permanent myocardial calcification. The clinical course was progressively downhill, and she died at 14 months of age. Neutralisation antibody to Coxsackie virus group B1 rose from 1:80 after delivery to 1:1280 at age 5 months. The protracted course of this infant's disease represents a new clinical form of fatal neonatal Coxsackie virus group B infection.

Calcinosis↗
PubMed1989

Spontaneous, gonadotropin-releasing hormone-induced, and progesterone-inhibited pulsatile secretion of human chorionic gonadotropin in the first trimester placenta in vitro.

Using a multichannel superfusion apparatus, the secretion of human chorionic gonadotropin (hCG) was measured at 1-6 minute intervals in placental explants at 7-9 weeks of gestation. hCG was found to be secreted in distinct pulses (4-5 fold above baseline) every 11-23 minutes peak to peak. Addition of one minute pulses of GnRH analog significantly increased, whereas pulses of progesterone decreased, pulsatile hCG secretion.

Chorionic Gonadotropin↗
PubMed1989

[Pefloxacin in the treatment of septicemia, purulent meningitis and salmonellosis].

Thirty two patients (16 female and 16 male), ranging in age from 13 to 80 years, were treated with pefloxacin (Abactal) at The Clinic of Infectious Diseases and Febrile Conditions. Pefloxacin was applied parenterally in 21 patients with serious infections. 15 of the cases had the signs of verified septicemia, i. e. bacteriemia; 5 patients developed purulent meningitis, i. e. meningoencephalitis; and in one patient bronchopneumonia occurred. Isolated causative agents (Staphylococcus aureus, S. epidermis and Gram-negative aerobic bacteria), were sensitive to pefloxacin. In 11 patients with enterocolitis Abactal was also included in the treatment because of the increasing occurrence of the resistance of Salmonella spp. to various chemotherapeutics and excellent "in vitro" effects of pefloxacin to those multiple-resistant species. Salmonella virchow was isolated from the stools of 6 patients. Salmonella enteritidis from 3 patients, Shigella sonnei from one, and in one case the causative agent was not identified. Pefloxacin was applied per os or parenterally in the dosage of 800-1200 mg in the period from 5-9 days. In all the patients (100%) eradication of the causative agents found in stools was done as early as the third or fourth day of the therapy. There were no adverse effects.

Adolescent↗
PubMed1989

Flow rate dependence of in vitro removal of anti-A and anti-B antibodies by immunoadsorbents with synthetic oligosaccharides representing blood group substances.

In vitro removal of anti-A and anti-B antibodies by immunoadsorbents with synthetic oligosaccharide structures representing blood group substances have been studied under controlled and simulated extracorporeal perfusion conditions at different flow rates ranging from 20 to 160 mL/min. There is a gradual decline in antibody titers in the plasma, as it relates to the antibody binding capacity and efficiency of the immunoadsorbents, both as a function of time and plasma volume at any of the flow rates employed. Approximately the same maximal binding capacity is achieved in about 4 hours of perfusion regardless of the flow rate, whereas increased number of plasma volumes are required at higher flow rates to compensate for the appropriate residence times needed in the column to utilize the same capacity. The perfusion time alone appears to be a critical factor which via the flow rate determines the residence time between the plasma and the immobilized hapten in the column. Also, the optimal flow rate at which maximum binding capacity of the immunoadsorbent may be utilized efficiently appears to fall within a range of the flow rate. The factors determining this range and the efficiency of the immunoadsorbents under dynamic conditions are discussed. The results obtained under the controlled conditions and the discussions that follow are expected to be generally useful, at least, as approximate clinical guidelines, for selective removal of specific substances by extracorporeal perfusion techniques utilizing therapeutic intervention with immunoadsorbents in this important area of medical therapy.

ABO Blood-Group System↗
PubMed1989

Investigation of age as a prognostic factor in early stage invasive cancer of the cervix. Implications for nursing.

A retrospective study was conducted investigating the relationships between age and length of survival, and between age and the prognostic factors of disease stage, tumor size, histologic grade, and hematocrit. The subjects consisted of all of the women with early-stage invasive squamous cell cancer of the cervix, treated with radiation therapy alone in the Department of Radiation Oncology at the State University of New York-Health Science Center at Brooklyn between January 1, 1980, and December 31, 1986. The 105 eligible subjects were divided into two groups: those 39 and younger (n = 22) and those older than 39 (n = 83). Length of survival was significantly shorter for the younger group (p = 0.0009). Of this group, 50% had died from disease compared with 23% of the older group. Tumor size was also significantly greater in the younger group (p = 0.038). Further study is needed to determine the contributing risk factors for cancer of the cervix in young women and to develop a comprehensive gynecologic nursing assessment tool for identifying those at increased risk for this disease. In terms of developmental psychology, the young women fit into the stage of early adulthood. Knowledge of the tasks of this stage would facilitate the development of primary cancer prevention programs for this group.

Adult↗
PubMed1989

Acquired immunodeficiency syndrome, a complication of cardiothoracic surgery.

We identified 13 patients who contracted acquired immunodeficiency syndrome or human immunodeficiency virus-related disease after a cardiothoracic operation. The operations were performed between January 1981 and November 1984, and the diagnosis of human immunodeficiency virus-related disease was established from 26 to 54 months after operation. The survival time from diagnosis ranged from 8 days to 14 months in the 10 patients who have died. A clinical illness developed in three of the patients immediately postoperatively that was consistent with primary human immunodeficiency virus mononucleosis. The clinical features included a wide variety of opportunistic infections, but an abnormally high percentage of the patients first showed symptoms of dementia or neoplastic disease. In many patients, the diagnosis was not suspected for a prolonged period of time. On the basis of the prolonged incubation period, the incidence of this disease is likely to increase for several more years.

Acquired Immunodeficiency Syndrome↗
PubMed1988

A method for the preparation of mononuclear cells devoid of platelet contamination and its application to the evaluation of putative alpha-receptors in normal and asthmatic subjects.

Receptor studies of human mononuclear leukocytes (MNLs) are complicated by the presence of contaminating platelets which have common receptors. A method was devised to produce MNLs free of platelets (less than 1%) and consists of sequential Ficoll-Hypaque gradients, a BSA gradient and a washing step. Lack of platelet contamination was confirmed by the following criteria: (a) microscopic evaluation using fluorescent dyes showed less than 1% platelets; (b) PGE1 stimulation of the leukocyte membrane adenylate cyclase required addition of exogenous GTP while the platelet cyclase did not; (c) immunoblots of the cells and membranes using antibodies strongly reactive against platelet membranes showed no reactivity against MNL membranes; (d) [3H]yohimbine showed no binding in MNL membranes under conditions where substantial binding to platelets was detected. MNLs were viable as judged by dye exclusion. PHA stimulation of lymphocytes was unimpaired. Plasma membranes of MNLs were prepared by brief sonication and fractionation on a sucrose step gradient. Binding studies using 3H-DHE, an alpha-receptor ligand, revealed no binding in MNLs from normal subjects (n = 6). By contrast, studies on cells from subjects with mild asthma with medication appropriately withheld (n = 8) showed low levels of binding (60-300 fmol/10(6) cells). The subtype and functionality of the putative alpha-receptors are being further evaluated.

Adenylyl Cyclases↗