Acidophilic protein crystals in lungs and bile ducts of helminth-infected mice.
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Biomedical subjects
Publications and source records attributed to M Kamiya.
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The ovicidal effects of heat and various chemical disinfectants on an oxyurid rat nematode Syphacia muris were investigated, using the hatching methods in artificial intestinal juice. The eggs were collected from the perianal skin of spontaneously infected rats by means of a piece of transparent adhesive tapes, and these eggs were treated with each disinfectant for two hours. It was found that 70% ethanol and 80 degrees C 30 min treatments killed almost all of the eggs. However, a small number of the eggs tested was killed by 0.02% chlorhexidine digluconate or 0.05% benzethonium chloride. Alcide, 3% saponated cresol solution, 50% isopropanol, 10 ppm sodium hypochlorite and 5 ppm iodophol had some effects against the eggs, but they didn't kill the eggs completely. A biological assay through infection of the eggs to rats might be necessary because the effects of 2% formalin on the eggs were not determined by the hatching methods.
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Larval counts were made on mice 2 days after oral inoculation with X-ray (0-320 Krad) or of gamma ray (0-6 Mrad) irradiated eggs containing second-stage Toxocara canis larvae. The majority of the larvae irradiated with 0-40 Krad were recovered from the liver and lungs, while most of the larvae irradiated with 80 or 160 Krad remained in the digestive tract, mainly in the stomach and the proximal half of the small intestine. Only a small number of the 320 Krad irradiated larvae was recovered from the mice. No significant difference was observed in the viability of irradiated larvae incubated in vitro up to 13 days after irradiation. However, a substantial percentage of the 160 and 320 Krad-irradiated larvae hatched during that period. Very few larvae were recovered from the digestive tract of mice inoculated with eggs irradiated with 0.5 Mrad, and only one and four larvae were recovered from the liver and lungs of a mouse. No visceral larval migration was observed in mice inoculated with 1 Mrad-irradiated eggs. The minimum lethal radiation dose for second-stage T. canis larvae in eggs is proposed to be 1 Mrad.
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Gnathostoma malaysiae Miyazaki and Dunn, 1965 was found in the stomach wall of Rattus surifer, captured in Phuket Island and Khao Yai National Park of Thailand. This is the first to be recorded in Thailand and the second discovery after the first description of Miyazaki and Dunn (1965). Pathological findings of infected animals were also described. Some new morphological descriptions were added to the original.
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