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Biomedical subjects

M Kamada

Publications and source records attributed to M Kamada.

At least 235 records · Page 13Linked to original sources
PubMed1985

[A clinico-pathological implication of ABO (H) blood group substances expressed in the primary lesion of uterine cervical carcinoma].

The disappearance of ABO(H) blood group antigens seen in the lesions of carcinoma of the uterine cervix has been studied by the Avidin-Biotin-Complex (ABC) method and the results have been correlated with histology and prognosis. The incidence of cases showing complete loss of A or B antigens was increased with progression in the pathological picture of cancer, whereas H antigen, known as precursor carbohydrate chains of A and B antigens, was still sustained even in most cases of invasive cancer. In the tissue of invasive cancer, the complete loss of H antigen was easily able to be demonstrated in the small cell non-keratinizing type of carcinoma, but it is hardly able to be seen in the large cell non-keratinizing type and not demonstrated in the keratinizing type of carcinoma. Though no relationship was recognized between the disappearance of A, B or H antigens and parametrial spread of carcinoma or metastasis to the pelvic lymphnodes, the incidence of patients who died within two years after operation was apparently higher in the H antigen negative group than in the H antigen positive group. It is concluded that the disappearance of A or B antigen may correlate with the invasiveness of the cancerous lesion, while that of the H antigen may reflect the grade of dedifferentiation of the neoplasia with the implication of an unfavorable prognosis.

ABO Blood-Group System↗
PubMed1984

Detection of anti-zona pellucida activities in human sera by the passive hemagglutination reaction.

A simple method of detecting anti-zona activities in human sera has been developed by passive hemagglutination reaction ( PHAR ) using bovine erythrocytes as indicator cells coated with purified porcine zona substance. By this test, 8 of 88 serum samples (9.1%) from infertile women gave positive reactions, whereas only 1 of 90 control sera (1.1%). A positive immunofluorescence (IF) assay confirmed the results of the PHAR in seven of the eight positive samples. Positive PHAR and IF on porcine zonae were abolished after adsorption with porcine erythrocytes, but IF on human zonae was retained by four of eight serum samples, indicating the presence of anti-zona autoantibodies in these infertile sera that were directed to an antigen(s) proper to human zonae.

Adult↗
PubMed1983

Analysis of small and large plaque variants of equine herpesvirus type 3 by restriction endonucleases. Brief report.

Six of 6 equine herpesvirus type 3 (EHV3) isolates, 5 of which were epidemiologically unrelated, produced a mixture of small and large plaque variants in equine foetal kidney cells under methylcellulose. In 4 of 4 instances the cleavage site(s) generating the Bam HI A fragment of large plaque variants was distinct from the site(s) for the same fragment of small plaque variants.

DNA Restriction Enzymes↗
PubMed1983

Comparative sequence analysis of the inverted terminal repetition in the genomes of animal and avian adenoviruses.

The nucleotide sequences at the inverted terminal repetitions from two animal adenoviruses, infectious canine hepatitis virus and equine adenovirus, and from one avian adenovirus, CELO, were analyzed. DNAs from infectious canine hepatitis virus and equine adenovirus contain a homologous region which is 23 nucleotides long from the terminus. The first 17 nucleotides of this region are identical to the ones in human adenovirus type 2 DNA. The striking homologous sequence of 14 nucleotides, conserved in the inverted terminal repetitions of several human adenovirus strains and in simian adenovirus type 7, is only partially conserved in the two animal and one avian adenoviruses reported here.

Adenoviridae↗
PubMed1983

A prospective study of congenital cytomegalovirus infection in Japan.

A prospective study on congenital cytomegalovirus (CMV) infection was carried out in Sapporo, Japan. Only 77 (6.2%) of 1233 pregnant women were seronegative in the first trimester. In such a highly immune population, 11 (0.5%) of 2070 newborn infants were found to have congenital CMV infection. None of them showed any clinical abnormalities during the period of observation (3-24 months), except for a cyst of septum pellucidum in one case. The incidence of congenital CMV infection in the first pregnancies was 1.1% (9/816) and in subsequent pregnancies 0.2% (2/1254). The difference was statistically significant. Three mothers of infants with congenital CMV infection were already seropositive in the first trimester. 26/30 (86.7%) infants, born to mothers with active CMV infection during pregnancy, had started to excrete CMV with urine before the age of 12 months. In contrast, none of the 4 infants born to seronegative mothers excreted CMV.

Adult↗
PubMed1982

Evolution of infectious mononucleosis into Epstein-Barr virus carrying monoclonal malignant lymphoma.

A monoclonal malignant lymphoma was diagnosed in a 30-month-old Japanese boy, who, since the age of 12 months, had had chronic, recurrent infectious mononucleosis manifested by repeated episodes of severe cough and high fever accompanied by marked lymphadenopathy and hepatosplenomegaly and high serum Epstein-Barr virus (EBV) antibody titres. The diagnosis of Burkitt's lymphoma was made when a cervical lymph-node biopsy specimen revealed massive proliferation of immature B-cells with starry-sky histiocytes. These lymph-node cells were characterised by a translocation between chromosomes 10 and 17. There were approximately 9 EBV genome-equivalents per cell. Most cells were positive for nuclear antigen (EBNA); early antigen (EA) and viral capsid antigen (VCA) were also detected. The presence of EBV was supported by finding that the cell-free lymph-node extract transformed cord-blood lymphocytes into EBNA-positive blast cells. The proportion of EA-positive and VCA-positive cells increased rapidly in culture for 24 h, then the positive cells degenerated rapidly and completely. The cells also contained numerous herpes-type virus particles. The child improved considerably with cytostatic treatment and has been in remission for 2 years.

Antibodies, Viral↗