Search PubMed⌕ Search

Biomedical subjects

M Jung

Publications and source records attributed to M Jung.

At least 343 records · Page 19Linked to original sources
PubMedDate not supplied

Cyclospora cayetanensis: first imported infections in Germany.

Over the last decade increasing numbers of enteritis cases have been attributed to infection with a new coccidian species that was named Cyclospora cayetanensis in 1993. Diarrhea caused by this agent is clinically indistinguishable from cryptosporidiosis, isosporiasis and microsporidiosis, but Cyclospora infections are often very prolonged (up to 15 weeks) and may cause severe weight loss. Diagnosis of infection is important because, in contrast to diarrhea caused by Cryptosporidium and microsporidia, treatment with co-trimoxazole is effective. Here we report the cases of two female patients, aged 70 and 58 years old, respectively, who suffered from severe, prolonged diarrhea after a vacation in Singapore, Java and Bali. Routine microbiological laboratory diagnostics were unable to demonstrate the presence of known enteropathogenic bacteria. Modified Ziehl-Neelsen staining of fecal smears revealed oocysts of Cyclospora cayetanensis, and treatment of the patients with co-trimoxazole was promptly effective. We would like to increase awareness of the possibility of Cyclospora infections in patients with prolonged diarrhea who have travelled to endemic areas.

Aged↗
PubMedDate not supplied

Idiotypic vaccine for treatment of human B-cell lymphoma. Construction of IgG variable regions from single malignant B cells.

Immunoglobulin idiotypes (Id) of malignant B cells represent highly specific markers which can be used for vaccination. PCR-amplification of immunoglobulin genes enables the rapid production of large amounts of Id vaccines. However, the separate amplification and subsequent recombination of heavy and light chains can lead to a loss of the relevant Id. To preserve the original chain pairs, we used single malignant B cells derived from an immunocytoma patient. Cytoplasm was extracted and the mRNA transcribed into cDNA. The VH and VL genes were then amplified by PCR and cloned into a vector for expression in E. coli. Id production was checked using an anti-Id mouse monoclonal Ab raised against the patient's tumor-specific IgG. One out of 3 constructs expressed the relevant Id. Analysis of the first 31 light chain residues revealed an identical sequence for the malignant B cells' IgG and the recombinant Id construct. Exchange of either the heavy or light chain with an unrelated chain resulted in loss of the Id. An unrelated sequence derived from the c-myc protein is coupled to the Id vaccine. The lymphoma patient was shown to have Abs to the c-myc sequence. This sequence therefore, increases the Id+ Ab's antigenicity. CD spectroscopy showed an alpha-helical structure for the c-myc epitope. In conclusion, a B-cell lymphoma autovaccine was produced containing immunogenic sequences that do not alter the steric conformation of the tumor-specific Id.

Animals↗