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M Jonas

Publications and source records attributed to M Jonas.

At least 73 records · Page 4Linked to original sources
PubMed1996

Simultaneous evaluation of fatty acid metabolism and myocardial flow in an explanted heart.

UNLABELLED: The biodistribution of the fatty acid analog [131I]PHIPA 3-10, was compared to the flow tracer 99mTc-sestamibi by quantitative analysis in a dual-isotope study performed during a heart transplantation. METHODS: Iodine-131-PHIPA 3-10 and 99mTc-sestamibi were injected simultaneously approximately 20 min prior to the start of surgical procedure. Scintigraphic images of the sliced explanted heart were compared to the preoperative in vivo scans using [123I]PHIPA 3-10, 201TI and 99mTc-sestamibi. In 14 tissue samples of the explanted heart, the radioactive contents from [131I]PHIPA 3-10 and 99mTc-sestamibi were calculated as %ID/g-values and correlated with the corresponding histology. RESULTS: In the preoperative scans, a mismatch of fatty acid uptake and perfusion ([123I]PHIPA 3-10 > flow) was observed which indicated residual viable myocardium, while a matched defect was associated with scar. In viable myocardium, there was a significantly higher accumulation of [131I]PHIPA 3-10 compared to 99mTc-sestamibi (mean 5.9 x 10(-3) versus 2.7 x 10(-3)%ID/g),whereas in scars the uptake of both tracers was comparable (1.2 x 10(-3) versus 1.4 x 10(-3)%ID/g). CONCLUSION: Myocardial viability can be defined more accurately with radioiodinated PHIPA 3-10 than with 99mTc-sestamibi. The differences of biodistribution in viable myocardium and scars indicate that not only perfusion but also the metabolic state of the myocardium can be evaluated with radioiodinated PHIPA 3-10.

Coronary Circulation↗
PubMed1995

Comparison of early invasive and conservative treatments in patients with anterior wall non-Q-wave acute myocardial infarction.

To compare the long-term prognosis of a group of patients treated by an early invasive approach after a non-Q-wave anterior wall acute myocardial infarction (AMI) with a similar group treated conservatively, data from 110 consecutive patients with non-Q-wave AMI were retrospectively obtained from 3 different hospitals: (1) a hospital with coronary angioplasty and coronary bypass facilities favoring on early invasive approach, (2) a hospital with a catheterization laboratory and no coronary angioplasty or coronary bypass facilities, and (3) a community hospital without a catheterization laboratory. Patients were divided according to the presence or absence of an early invasive approach: those who had undergone in-hospital catheterization and revascularization (n = 55) and those with a conservative approach (n = 55). The early invasive approach resulted in a significant decrease in major events. The rate of recurrent myocardial infarction was 29% in the conservative group versus 7.2% in the invasive group (p = 0.025). Survival rate curves at 3-year follow-up showed significant differences in mortality (p = 0.001), recurrent myocardial infarction (p = 0.002), recurrent angina pectoris (p = 0.001), and development of congestive heart failure (p = 0.05). Multivariate analysis disclosed the early invasive approach to be an independent predictor for decreasing the likelihood of recurrent infarction by 86% (odds ratio 0.14, confidence intervals 0.04 to 0.48, p = 0.0006), and for decreasing the likelihood of recurrent angina by 66% (odds ratio 0.34, confidence intervals 0.18 to 0.63, p < 0.005). The early invasive strategy may result in an improved outcome in the treatment of patients with non-Q-wave anterior wall AMI compared with patients treated conservatively.

Aged↗
PubMed1995

Translocation of the 85-kDa phospholipase A2 from cytosol to the nuclear envelope in rat basophilic leukemia cells stimulated with calcium ionophore or IgE/antigen.

The rat mast cell line RBL-2H3.1 contains an 85-kDa cytosolic phospholipase A2 (cPLA2) that is very likely involved in liberating arachidonate from membrane phospholipid for the synthesis of eicosanoids following stimulation with either calcium ionophore or IgE/antigen. In this study, the intracellular location of cPLA2 was determined using immunofluorescence microscopy and immuno-gold electron microscopy. In nonstimulated cells, cPLA2 is distributed throughout the cytosol and is excluded from the nucleoplasm. Following cell activation with calcium ionophore, most of the cPLA2 translocates to the nuclear envelope, and the enzyme remains there during the entire period that ionophore is present. With IgE/antigen stimulation for 5 min, approximately 20-30% of the cPLA2 translocates to the nuclear envelope, and after 30 min of stimulation, most of the enzyme returns to the cytosol. Measurement of intracellular calcium using the dye Fura-2/AM shows that the level of calcium rises immediately after antigen is added, remains high for about 30 s, and then declines back to resting levels. Activation with calcium ionophore produces a 10-fold larger release of arachidonate than does stimulation with IgE/antigen. Thus, the results suggest that the extent of membrane binding of cPLA2 correlates with the release of arachidonate and that the site of arachidonate liberation is the nuclear envelope where many of the enzymes that oxygenate this fatty acid are located.

Animals↗
PubMed1995

[67Ga scintigraphy in acute sarcoidosis with Heerfordt's syndrome].

Sarcoidosis is a chronic multisystem disorder of unknown etiology characterized by non-caseating epitheloid granulomas. We report a case of a 29-year old female with acute sarcoidosis and Heerfordt's syndrome (parotid enlargement, anterior uveitis and facial nerve palsy) who had a typical clinical constellation but no histologic criteria of a granulomatous inflammatory process in transbronchial lung biopsy. 67Ga scintigraphy revealed such a typical pattern of accumulation that sarcoidosis could be diagnosed despite negative histological findings.

Adult↗
PubMed1994

Using "unsuitable" hearts for transplantation.

Donor availability is the single most limiting factor in heart transplantation. From a consecutive series of 100 heart donors, there were 21 which fell well outside our minimum criteria on initial inspection: mean arterial pressure (MAP) more than 60 mm Hg, central venous pressure (CVP) less than 12 mm Hg, pulmonary capillary wedge pressure (PCWP) less than 12 mm Hg, left ventricular stroke work index (LVSWI) more than 15 g.m. on inotropes less than 5 mcg/kg per min. Of these 13 out of 21 had a MAP less than 55 mm Hg, 6 out of 21 a CVP more than 15 mm Hg and 2 out of 21 were on inotropes at more than 20 mcg/kg per min. Following full invasive monitoring another 14 donors fell outside our criteria; 5 had a mean LVSWI of 12.4 g.m. and 9 had a mean PCWP of 19.6 mm Hg. Following the institution of our hormone-based pharmacological resuscitation regime 30 of these donors yielded 19 transplantable hearts and 11 transplantable heart-lung blocks. The other five were not used due to left ventricular hypertrophy (2), inotrope dependency (2) and persistent poor function (1). Twenty-five of the 30 recipients of these organs (83.3%) are alive and well, 4-25 months post transplant. Four early deaths occurred; one arrhythmia (heart), one acute respiratory distress syndrome (heart), one cerebrovascular accident (heart lung) and one infection (heart, lung and liver). One death occurred at 90 days from tamponade (heart). Aggressive and focussed donor management has helped us to maintain our levels of transplant activity, without compromising the outcome--a 30-day mortality of 16.2% in 1989, 11.8% in 1990 and 6.8% in 1991.

Blood Pressure↗
PubMed1993

Hepatic retransplantation in New England--a regional experience and survival model.

Hepatic retransplantation (reTx) offers the only alternative to death for patients who have failed primary hepatic transplantation (PTx). Assuming a finite number of donor organs, reTx also denies the chance of survival for some patients awaiting PTx. The impact of reTx on overall survival (i.e., the survival of all candidates for transplantation) must therefore be clarified. Between 1983 and 1991, 651 patients from the New England Organ Bank underwent liver transplantation, and 73 reTx were performed in 71 patients (11% reTx rate). The 1-year actuarial survival for reTx (48%) was significantly less than for PTx (70%, P < 0.05). This survival varied, dependent on the interval of time following PTx in which the reTx was performed (0-3 days, 57% survival; 4-30 days, 24%; 30-365 days, 54%; and > 365 days, 83%). Patients on the regional waiting list had an 18% mortality rate while awaiting transplantation. These results were incorporated into a mathematical model describing survival as a function of reTx rate, assuming a limited supply of donor livers. ReTx improves the 1-year survival rate for patients undergoing PTx but decreases overall survival (survival of all candidates) for liver transplantation. In the current era of persistently insufficient donor numbers, strategies based on minimizing the use of reTx, especially in the case of patients in whom chances of success are minimal, will result in the best overall rate of patient survival.

Adolescent↗
PubMed1993

I-band periodicity in rabbit psoas muscle fibers measured using digital image-analysis techniques.

The fine periodicity of I-band was measured in single fibers of glycerinated rabbit psoas muscle, in relaxed, activated and rigor states at short (2.5 microns) and long (3.2 microns) sarcomere lengths. Measurements were carried out using fast Fourier transforms obtained from I-band regions of digitized electron micrographic images. The mean periodicity, averaged among all conditions, was 42.5 +/- 1.5 nm. Some differences were found among the different conditions, but they were too small to be conclusive. The observed periodicity value was considerably higher than the generally assumed value of 38.5 nm, and is surprisingly close to the myosin-repeat spacing.

Actins↗
PubMed1992

Pulmonary damage after intramedullary femoral nailing in traumatized sheep--is there an effect from different nailing methods?

Stabilization of femoral shaft fractures is a controversial issue in the management of patients with multiple trauma. Intramedullary nailing usually is preferred primarily; in recent years, however, pulmonary complications (e.g., ARDS) have been reported that were attributed to the reaming procedure. To study the effects of different nailing methods in a model of severe trauma, hemorrhagic shock and lung contusion were created at day 1 in sheep prepared by the method described by Staub. After recuperation (day 3) the animals in the study group (group 1) underwent intramedullary nailing of a closed femur without prior reaming; group 2 was treated with reaming and nailing according to AO standards. The reaming procedure led to an acute increase of pulmonary arterial pressure only in group 2 (19.8 +/- 2.1 to 31.0 +/- 4.6 mm Hg). Pulmonary triglyceride levels increased at parallel time points from 18.27 +/- 2.3 to 33.04 +/- 7.37 mg/dL only in group 2. Stimulatory capacity of polymorphonuclear leukocytes (PMNL) increased in the study group and decreased in controls (group 1: 2.652 +/- 0.23 x 10(6) cpm to 3.387 +/- 1.34 x 10(6) cpm; group 2: 2.699 +/- 0.34 x 10(6) cpm to 2.460 +/- 0.187 x 10(6) cpm). Intramedullary nailing caused an increase of lung capillary permeability in both groups; in the study group less damage was seen (group 1: 0.390 +/- 0.0006 to 0.354 +/- 0.011; group 2: 0.391 +/- 0.0004 to 0.336 +/- 0.015; p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Acetylglucosaminidase↗
PubMed1992

[99mTc-MAB bone marrow scintigraphy and probe-guided biopsy in aplastic anemia].

The diagnosis of systemic haematological disorders is often confirmed by bone marrow biopsy which is usually performed "blind". False-negative results may be obtained because this method is restricted to a small part of the blood-forming organ. Bone marrow scintigraphy has the potential of detecting the distribution of active haematopoietic bone marrow and to localize infiltrative lesions. This case report of a patient with pancytopenia demonstrates that the diagnosis of aplastic anaemia can be confirmed by bone marrow scintigraphy in combination with probe-guided bone marrow biopsy.

Aged↗
PubMed1991

[Does lung contusion and general injury severity have an effect on the lung following intramedullary femoral nailing? An animal model].

We evaluated changes in lung function after closed intramedullary femoral nailing (IMN) in sheep. The effects of isolated IMN were compared with those of nailing after lung contusion and hemorrhagic shock. In adult female merino sheep a chronic lung lymph fistula was prepared according to the method described by Staub. At day 1, group 1 received right-sided lung contusion and hemorrhagic shock to a mean blood pressure of 50 mmHg for 2 h. Group 2 was the control group. At day 3 both groups were submitted to IMN, followed by a 2-h observation period. IMN caused a transient significant increase in pulmonary artery pressure and central venous triglycerides in both groups. Chemiluminescence of isolated polymorphonuclear leukocytes (PMNL) decreased in group 1 from 2.699 +/- 0.344 to 2.460 +/- 0.187 x 10(6) cpm/25000 PMNL and increased in group 2 from 2.757 +/- 0.127 to 3.824 +/- 0.439 x 10(6) cpm/25000 PMNL. Lymph flow in group 1 increased 1.5-fold while microvascular pressure decreased. In group 2 lymph flow increased less, while MVP increased. The filtration coefficient in group 1 was 5 times (7.533 +/- 0.044) that in the control group (1.45 +/- 0.133). Calculations of permeability indicated a 2-fold increase (0.044 group 1 vs 0.026 group 2). In the presence of previous lung contusion and hemorrhagic shock there is definite lung damage from IMN. This is probably mediated by stimulation of PMNL.

Animals↗
PubMed1990

Benign pneumatosis intestinalis with subcutaneous emphysema in a liver transplant recipient.

Pneumatosis intestinalis (PI) occurred in a pediatric liver transplant recipient experiencing chronic rejection. Signs and symptoms included abdominal distention, subcutaneous emphysema, fever, and malaise. Antibiotic treatment and nasogastric decompression resulted in prompt relief of symptoms, and surgery was not necessary. The etiology, pathophysiology, and therapy of this rare condition are discussed.

Child, Preschool↗