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Biomedical subjects

M Jiang

Publications and source records attributed to M Jiang.

At least 235 records · Page 13Linked to original sources
PubMed1988

[Inhibitory effect of pingyangmycin on human liver cancer and stomach cancer xenografts in nude mice].

Established human cancer cell lines derived from liver cancer (BEL-7402), nasopharyngeal cancer (CNE-2) and stomach cancer (MGC-803) were used in this study. These human cancer cells have been serially transplanted in nude mice. As determined by clonogenic assay, pingyangmycin (bleomycin A5) was highly cytotoxic to these three cell lines, of which the liver cancer cell line was more sensitive than the other two. At a tolerated dose level, pingyangmycin exhibited a remarkable growth inhibition on the growth of the liver cancer and stomach cancer xenografts, the inhibition rates being 70% and 85%, respectively. No pathologic changes were found in the organs of treated animals.

Adenocarcinoma, Mucinous↗
PubMed1987

Some biochemical characteristics and cell membrane actions of a toxic phospholipase A2 isolated from the venom of the pit viper Agkistrodon halys (Pallas).

A toxic component (AgTx) from the venom of Agkistrodon halys (Pallas) was isolated using DEAE-cellulose DE11 and CM-Sephadex C50 column chromatography and finally purified to homogeneity by FPLC on a MonoQ column. The toxin is a neutral (pI 6.9) single chain polypeptide with a mol. wt of 14,000 and an amino acid composition (123 residues) roughly similar to that of notexin. AgTx was found to have phospholipase A2 activity which was dependent on calcium and stimulated by sodium deoxycholate. The toxin caused efflux of 2-deoxy-(1-3H)-glucose-6-phosphate (a cell membrane integrity probe) as well as of [3H]acetylcholine from rat brain synaptosomes. No cell membrane damage was induced by AgTx on cultured N1E 115 neuroblastoma cells and chick myotube cultures. The LD50 ws 150 micrograms/kg (i.p.) in mice. The main symptom observed was respiratory paralysis. The results obtained show that AgTx can be classified as a toxic phospholipase A2 with a presynaptic site of action.

Acetylcholine↗
PubMed1987

Isolation and pharmacological characterization of a new alpha-neurotoxin (alpha-AgTx) from venom of the viper Agkistrodon halys (Pallas).

A hitherto unknown alpha-neurotoxin, alpha-agkistrodotoxin, was isolated from the venom of the pit viper Agkistrodon halys (Pallas). It's molecular weight was approx. 8000 +/- 80 (SDS-polyacrylamide electrophoresis). The toxin crossreacted with antiserum directed against alpha-bungarotoxin and inhibited binding of 125I-alpha-bungarotoxin to the nicotinic acetylcholine receptor of cultured myotubes (IC50 = 2 X 10(-9) M). The association and dissociation rates were 4.85 X 10(5) per mole per min and 3.55 X 10(-4) per min, respectively, giving a Kd of 7.3 X 10(-10) M. The toxin also inhibited carbachol-induced influx of cations through the nAChR (IC50 = 6 X 10(-8) M).

Animals↗