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Biomedical subjects

M Jean

Publications and source records attributed to M Jean.

36 records · Page 2Linked to original sources
PubMed1995

[Atrial insertion of accessory pathways in permanent reciprocating junctional tachycardia].

Permanent reciprocating junctional tachycardia (PRJT) is an uncommon type of Tachycardia, usually associated with a retrogradely conducting accessory pathway situated near to the ostium of the coronary sinus. This study reports the localisation of the site of atrial insertion of the accessory pathway, confirmed by the efficacy of intracardiac catheter ablation. Five patients (4 men), aged 14 to 45, experienced PRTJT at rates of 120 to 150/mn over a period of 2 to 15 years. 4 patients were normal, expected in 2 patients in whom they were 16 and 20%. One of these suffered a thromboembolic complication after pharmacological interruption of the tachycardia. The presence of an accessory pathway with decremential retrograde conduction was confirmed in all cases. Catheter ablation was successful in the medio-septal (2 cases), posteroseptal (1 case), lateral (1 case) and anteroseptal (1 case) regions. Six to 30 applications of 20 to 30 watts of radiofrequency energy were used in the 5 cases. In one case (right lateral accessory pathway), a fulguration procedure was necessary with a cathodic shock of 160 Joules. The AV conduction was preserved and retrograde conduction was normalised in all cases. All patients remained asymptomatic for a period of 10 to 43 months without antiarrhythmic therapy. The ejection fractions of the two patients with left ventricular dysfunction returned to normal. The authors conclude that the accessory pathways of PRJT may be situated in different regions of the right atrium. The efficacy of catheter ablation was 100 % in this form of tachycardia.

Adolescent↗
PubMed1994

A fucose-containing epitope potentially involved in gamete interaction on the human zona pellucida.

The oligosaccharide moiety of human, porcine and bovine zonae pellucidae was studied with lectins and monoclonal antibodies specific for tri- or tetra-saccharidic epitopes containing at least one terminal alpha-L-fucose. Animal eggs were collected from follicular aspirates, human eggs were collected from in-vitro fertilization and embryo transfer programmes and pooled into six groups. By direct immunofluorescence, the lectins reactivity was detected for the animal or the human zonae pools in the same way. Reactivity of Aleuria aurantia lectin demonstrated the presence of alpha-L-fucose terminal residues in the zonae from the three species studied. By indirect immunofluorescence, the 2-25 antibody reactivity was detected in every pool of human zonae whereas there was no evidence of any antibody reactivity on animal zonae. Using an anti-Lewis-b blood group antibody (2-25), we observed expression of this antigen as an intrinsic component of the human zona pellucida, independently of patients' Lewis red blood cell phenotypes. Antibody 2-25 inhibited the spermatozoa-zona binding in a hemizona assay, suggesting that this fucose-containing antigen could be part of a sperm-zona receptor.

Animals↗
PubMed1994

[Electrocardiographic evaluation of anti-ischemic effect of verapamil during angioplasty].

The anti-ischemic and cardioprotective effects of various angina drugs have been evaluated during angioplasty. This special situation of transitory regional ischemia was used to test verapami given by intra-coronary injection in 15 patients undergoing balloon dilatation of the left anterior descending artery, suffering from stable angina and with normal left ventricular kinetics. Each patient serving as his own control, parameters indicating oxygen consumption and clinical and electrocardiographic (ST segment changes) markers were measured during two successive inflations. Continuous monitoring of comparative variations between a first inflation without treatment and a second inflation after intra-coronary administration of 1 mg of verapamil revealed, following 60 seconds of ischemia, the absence of any change in heart rate, a moderate fall in mean blood pressure (101.7 as against 96.8 mmHg, p = 0.033) and a notable 60% reduction in the amplitude of maximal ST depression (5.07 +/- 3.08 mm as against 2.07 +/- 1.44 mm, p = 0.0002). These results confirm the anti-ischemic properties of verapamil, the action of which is based upon an indirect peripheral vasodilator model but also on a probable direct cardioprotective effect on anoxic myocardial regions.

Adult↗
PubMed1993

[Future of the treatment of male infertility].

The aim of this paper is to introduce inherent difficulties leading to relative delay in the field of male infecundity treatment. Four theoretical possibilities are exposed: infertility prevention improvement, qualitative or quantitative improvement of spermatogenesis, spermatozoa fecundity ability improvement and micro-fertilization procedures. These methods are exposed in their technical aspects and in their results. Critical analysis is conducted about some impressive reported series. Basic knowledge improvement seems to be necessary to explore new therapeutic ways.

Fertilization in Vitro↗
PubMed1993

Medical abortion after in vitro assisted fertilization.

Subzonal sperm insertion is an assisted insemination procedure to enhance IVF in patients with severe male factor. We report a lethal fetal abnormality that occurred in a SUZI-obtained pregnancy. It is essential that such cases be extensively collected to assess the feasibility of using this method in patients with severe semen defects.

Abortion, Induced↗
PubMed1993

[Cancer of the ovary and medically assisted reproduction].

Case reports and epidemiologic studies have recently indicated a possible link between ovarian stimulation, ovarian cancer and ovarian tumours. We sent a questionnaire to 116 French IVF centers to determine whether ovarian cancer had occurred in women treated during the last 10 years. The 19 cases of ovarian cancer reported have since been studied more thoroughly in a follow up questionnaire. Six can now be excluded, and the 13 remaining cases are compared. Five of these patients had fewer than 4 stimulation cycles here. Imputability was considered probable for 2 patients, possible for 5 patients, improbable for 3 and highly improbable for 3. Histologically, 6 (46%) cases involved borderline lesions. These results indicate that there is no need to change medical practice, but we are convinced that prospective epidemiologic research should be carried out immediately.

Adult↗
PubMed1993

[The bovine zona pellucida: differences in macromolecular composition between oocytes, pre-treated with A-23187 or not, and embryos].

The SDS-PAGE method was used to determine the composition of isolated bovine zona pellucida (ZP). This egg extracellular coat appears to be characterized by 3 major glycoproteins (ZP1, ZP2, ZP3), with apparent molecular weight (MW) of 80-70 kD, 66-63 kD and 60 kD, respectively, as revealed by 1-dimensional SDS-PAGE. After 2-dimensional SDS-PAGE, the zona pellucida electrophoretic pattern indicates a fourth glycoprotein, thus called ZP4. SDS-PAGE analysis of ZP isolated from oocytes and embryos after transit through the genital tract of A23187 pretreated oocytes allowed the description of modifications in glycoproteinic composition.

Animals↗
PubMed1992

Utility of zona pellucida drilling in cases of severe semen alterations in man.

OBJECTIVE: To evaluate the utility of zona drilling for human severe sperm alterations in in vitro fertilization (IVF). DESIGN: A prospective randomized study was conducted according to random assignment of collected oocytes to a control or zona drilling procedure. SETTING: Reproductive Biology Laboratory, University Hospital of Nantes, France. PATIENTS: Twenty infertile couples with severe semen defects and previous IVF failure. INTERVENTIONS: Ultrasound-guided transvaginal follicular aspiration. RESULTS: Fertilization rates were compared to assess the advantages of zona drilling: 93% (80/86) drilled oocytes survived and 18.75% (15) were fertilized, whereas only 3% (3/100) control oocytes were fertilized. The polyspermy rate for fertilized drilled oocytes was high (10/15; 66.6%). The normal fertilization rate after zona drilling remained very low (5/86; 5.8%) and was not statistically different from that of control oocytes (3/100; 3%). One implantation occurred after replacement of five drilled embryos in three patients but resulted in early miscarriage. Replacement of the single control embryo led to a progressive pregnancy and normal male birth. CONCLUSIONS: This study indicates that zona drilling does not improve IVF results in cases of severe semen alterations.

Female↗
PubMed1985

[Etiology of bacterial vaginosis (non-specific vaginitis)].

56 women who were diagnosed bioclinically as having a bacterial vaginal infection were studied, as were 35 women as a control group. The study was a semi-quantitative analysis of the vaginal bacterial flora, both aerobic and anaerobic. It shows that Gardnerella vaginalis and anaerobic bacteria such as Peptococcus, Peptostreptococcus, Bacteroïdes, Veillonella and Mobiluncus were associated in a statistically significant way with bacterial vaginitis. On the other hand Lactobacilli were less frequently found (p less than 0.001) than in the control group of women. The way in which the microbial flora is changed has been observed during attacks of vaginitis and is discussed, as is the importance of making the diagnosis and of treating this syndrome.

Bacterial Infections↗
PubMedDate not supplied

[Microfertilization techniques: con].

The actually used microfertilization procedures are exposed according to potential indications: flagellar immotility, previous unexplained failure of IVF, and oligo-asthenoteratozoospermia. Results of two prospective randomized studies are presented in case of severe asthenozoospermia associated with at least one previous failure of IVF. Evaluation of fertilization rates confirms that ZD does not improve IVF prognosis whereas SUZI could be used to enhance IVF results. Authors focused on major methodological criticisms raising about most of published series, on relatively low evolutive pregnancy rate (5.7%) and on the need of randomized prospective studies for judge real interest of assisted fertilization.

Female↗