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Biomedical subjects

M Janssens

Publications and source records attributed to M Janssens.

At least 55 records · Page 3Linked to original sources

Identification of vancomycin-resistant lactic bacteria isolated from humans.

By using cell morphology, arginine dihydrolase, and gas production in de Man, Sharp, Rogosa broth, 122 isolates of vancomycin-resistant lactic bacteria from humans were assigned to five profiles, allowing us to distinguish Pediococcus, homofermentative and heterofermentative Lactobacillus, and Leuconostoc species. The absence of L-(+)-lactic acid, as detected spectrophotometrically, was confirmatory for Leuconostoc species. API 50 CHL panels were useful for the identification of Lactobacillus species.

Drug Resistance, Microbial↗

Laryngeal mask.

The laryngeal mask, provides a totally patent airway when positioned in the hypopharynx with remarkable patient tolerance, even at very light planes of sedation. The major advantages of the laryngeal mask are its ease of insertion, the absence of contact with the vocal cards, and the fact that it frees the hands of the anesthesiologist. Contraindications to its use result from its failure to seal the airway against regurgitation of gastric content. Laryngeal masks can be used easily instead of facial masks during anesthesia with spontaneous ventilation and, with experience, can be used for longer procedures using controlled ventilation. Suspected difficult intubation and establishment of a patent airway in emergency conditions are good indications for the use of this device. The laryngeal mask does not replace endotracheal intubation. It can, however, permit better management of the airway while waiting for personnel trained in endotracheal intubation. The nature of the pulmonary pathology seen in intensive care patients limits use of the laryngeal mask during intensive care. In the operating room few complications have been described, and postoperative discomfort is minimal. The laryngeal mask is a device positioned in the hypopharynx which allows separation of the digestive tract from the airway, without violation of either the larynx or the upper oesophageal sphincter. An endotracheal tube, because of its positioning, hinders normal glottic movement and narrows the airway.(ABSTRACT TRUNCATED AT 250 WORDS)

Anesthesia, Inhalation↗

Efficacy of levocabastine in conjunctival provocation studies.

Levocabastine is a new topical histamine H1 antagonist. The antihistaminic and antiallergic effects of levocabastine eye drops have been evaluated in eight conjuctival provocation studies (n = 238). Two studies used a histamine challenge; five studies used allergen challenge; one study used both and in one study allergic provocation was with compound 48/80. In all but one study, only one single dose of levocabastine (one or two drops) was given. Six studies were against placebo only; one was against cromoglycate and one study used both placebo and cromoglycate as reference drugs. Single instillation of levocabastine eye drops protected against histamine and allergen-induced ocular symptoms within a period of 10 minutes. Levocabastine eye drops significantly alleviated conjunctival itching, redness, chemosis, eyelid swelling and tearing induced by histamine or allergen challenge (p < or = 0.05). Four hours after administration levocabastine was still active. With levocabastine, but not with cromoglycate, a significant increase was observed in the allergen threshold. Even when compared to cromoglycate given as a pre-treatment four times daily for two weeks before the allergen challenge, a single dose of levocabastine was significantly more effective in inhibiting hyperaemia, eyelid swelling, chemosis and tearing (all p < 0.05). In conclusion, conjunctival provocation studies have established that levocabastine has a rapid and long-lasting effect in protecting against histamine or allergen-induced conjunctival symptoms.

Conjunctivitis, Allergic↗

New trends in the treatment of allergic conjunctivitis.

Histamine is the key mediator producing itching, redness and chemosis in allergic conjunctivitis. Histamine levels in tears are increased ten-fold in patients with this allergic condition. Levocabastine is a newly synthesized histamine H1 antagonist which has been formulated as both eye drops and nasal spray. In well established assays of antihistamine activity, levocabastine was found to be the most potent antihistamine compound available, being 15,000 times more potent than chlorpheniramine. Ocular provocation studies in man have shown that levocabastine protects against the symptoms of allergen-induced conjunctivitis. Ophthalmological examinations, including slit lamp and ophthalmoscopy showed no adverse effects. Data from therapeutic studies are available for more than 1700 patients with allergic conjunctivitis treated for 2-16 weeks. One drop of levocabastine (0.5 mg/ml) per eye given two to four times daily provided significantly better symptom control than placebo, with good to excellent results in 71% of patients on levocabastine compared to 55% on placebo (p < 0.001). Levocabastine has a fast onset of action. In one study 94% of patients experienced symptom relief within 15 minutes after the first instillation. The effects observed with levocabastine were at least as good as those with ocular cromoglycate or oral terfenadine. The incidence of adverse experiences was not different from placebo. Levocabastine promises to be a valuable treatment for patients with allergic conjunctivitis.

Animals↗

Isolation of Arcanobacterium haemolyticum from patients with pharyngitis in Belgium.

During a 40 months period, 49 Arcanobacterium haemolyticum strains were isolated from 12,000 throat swabs of patients with pharyngitis originating from 5 belgian provinces. All strains were recovered on sheep blood Columbia agar plates, supplemented by nalidixic acid and colistin. In two thirds of the cases, pharyngitis was associated with a rash. Half of the cases occurred in the age group between 10 and 20 years and isolates were twice more frequent in females than in males. Streptococcus pyogenes was isolated 23.2 times more frequently than Arcanobacterium haemolyticum.

Adolescent↗

Ketanserin in the treatment of peripheral vascular disease or hypertension in patients with diabetes mellitus: a review.

The available data on the use of ketanserin in diabetes mellitus have been reviewed. Ketanserin does not worsen glucose tolerance or destabilize diabetic control. Ketanserin can improve impairment of peripheral blood flow, and especially skin blood flow; the healing of cutaneous ulcers is improved; trophic skin changes are fewer; and there is a trend towards less limb amputations. Walking distance in diabetic claudicants is unaltered by ketanserin. Controlled trials in hypertensive diabetics have provided variable results, with a significant fall in arterial pressure in only one of five studies. As in nondiabetics, ketanserin generally lowers serum cholesterol. The electrocardiographic QTc interval is prolonged. Symptomatic side effects in diabetics are as those in nondiabetic subjects.

Adult↗

The laryngeal mask--Liège experience.

The laryngeal mask (LM), a recently commercialized device, provides a totally patent airway when positioned in the hypopharynx. The major advantages of the LM are its ease of insertion, the absence of contact with the vocal cords, and the fact that if frees the hands of the anesthesiologist. Contraindications to its use result from its failure to seal the airway against regurgitation of gastric content. After reviewing the modalities of using the LM, we describe the Liège experience with it. Over two months, 107 anesthetics were administered with the LM, 80 with spontaneous respiration, 22 with controlled ventilation and 5 where the ventilatory mode was changed intraoperatively. 84% of LM were inserted on the first attempt, 14% on the second. The use of the LM was unsuccessful three times. Only 13% of patients complained of mild pharyngeal discomfort postoperatively. We conclude that this device is reliable, easy to use, and causes minimal postoperative problems. Its use, for both spontaneous and controlled ventilation, is increasing in our hospital.

Adolescent↗

The differential efficacy of antihypertensive agents in the elderly.

A number of controlled trials have shown that antihypertensive therapy is beneficial to the elderly (greater than or equal to 60 years of age). Even so, there may be deleterious age-related effects associated with such therapy. Because of physiological and pathophysiological problems peculiar to the elderly (many of which remain to be defined precisely), both benefits and untoward effects of antihypertensive drugs differ, sometimes within classes of agents themselves. None of the traditional agents is ideal for the management of hypertension in the elderly. Differential efficacy and tolerability of various antihypertensive agents has often been claimed but none of these claims appears established beyond dispute. Increasing evidence, however, suggests that the serotonin antagonist ketanserin may be more effective and better tolerated in elderly hypertensives. If the elderly are to derive maximum benefit from antihypertensive therapy, the question of age-related differential effects of agents used needs to be addressed in carefully designed studies to determine the nature and magnitude of such effects, where they exist and what clinical implications they may have.

Aged↗

New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork.

A new enrichment medium for the recovery of pathogenic Yersinia enterocolitica serogroup O:3 from naturally infected meat products based on three selective agents, Irgasan, ticarcillin, and potassium chlorate (ITC), was compared with several other one- or two-step enrichments. Y. enterocolitica serogroup O:3 was recovered from 96.5% of 29 pork tongues, 24% of 50 ground pork samples, 16% of 25 masseter muscle samples, and 61% of tonsils. ITC was by far the most sensitive method for the recovery of Y. enterocolitica O:3, especially from ground meat and masseter muscles, while cold and two-step enrichments yielded better results for nonpathogenic strains. Plating of ITC enrichments onto SS-deoxycholate-calcium agar gave overall better results than plating onto cefsulodin-Irgasan-novobiocin agar for serogroup O:3.

Animals↗

Ketanserin compared to nifedipine and methyldopa in patients aged above 50 years: two international multicentre studies. For the International Study Group.

The antihypertensive properties and adverse reactions of ketanserin, nifedipine and methyldopa were compared in hypertensive patients aged above 50 years in two international studies. After a placebo run-in period, patients received for 3 months, either ketanserin or nifedipine retard in study 1, and either ketanserin or methyldopa in study 2. After 1 month of monotherapy, a diuretic was added if necessary. Study 1 had 117 subjects, with 119 in study 2. The changes in systolic and diastolic blood pressure were similar for ketanserin and nifedipine although more patients switched to combination therapy in the ketanserin group. The overall response rate was comparable with ketanserin and nifedipine (96% for both drugs). The decrease in diastolic blood pressure was significantly greater in the ketanserin group compared to methyldopa and more patients responded to ketanserin (82%) than to methyldopa (65%). No rebound hypertension was observed on discontinuation of therapy with any of the drugs. The total incidence of adverse reactions in monotherapy was lower with ketanserin than with nifedipine (34 and 47%) and similar for ketanserin and methyldopa (40 and 45%). For ketanserin no consistent adverse reaction pattern was seen.

Blood Pressure↗

Determination of sorbitol and galactitol at the nanogram level in biological samples by high-performance liquid chromatography.

Aldose reductase is an enzyme that plays an important role in diabetic complications such as cataract and neuropathy. The best way to estimate the enzymatic activity of this enzyme in vivo consists of measuring the accumulation of sorbitol or galactitol in various types of cells or tissues. A sensitive method to measure the polyols in biological samples by high-performance liquid chromatography has been developed. This method is based on the fact that polyols like sorbitol and galactitol react with phenylisocyanate to yield uv-absorbing derivatives at 240 nm. Applications to the separation and determination of polyols in biological samples of various origins, such as lenses, sciatic nerves, human skin fibroblasts, and red cells, are described and illustrated.

Animals↗