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M Jankowski

Publications and source records attributed to M Jankowski.

At least 73 records · Page 4Linked to original sources
PubMed1993

The Vin-1 gene, identified by provirus insertional mutagenesis, is the cyclin D2.

The Vin-1 gene was initially identified as a gene whose expression is altered by the integration of proviruses in the Vin-1 common site of integration in retrovirus-induced rodent T-cell leukemias. We have now isolated the Vin-1 cDNA. Sequencing of the Vin-1 cDNA and Vin-1 exons revealed that the proviruses are integrated at the 5' end of the Vin-1 gene in an inverse transcriptional orientation. The sequence of the Vin-1 gene is identical to that of the recently identified G1-phase cyclin D2 gene. The human homolog of the Vin-1/cyclin D2 gene (CCND2) was mapped to chromosome 12, band p13.3, by in situ hybridization, confirming previous mapping data. Our results strongly support a role of the cyclin D2 gene in oncogenesis and thereby implicate altered cell cycle regulation in transformation.

Amino Acid Sequence↗
PubMed1991

[Usefulness of ELISA and radial immunodiffusion tests for evaluation of the degree of purification of influenza diagnostic and vaccine preparations].

It is necessary to use new diagnostic tests for careful and rapid evaluation of a degree of purification and immunogenicity of vaccine anti-influenza preparations. In this study in order to obtain this purpose a radial immunodiffusion++ test and immunoenzymatic test (ELISA) were used Recommended by WHO radial immunodiffusion++ test enable to determine a level of haemagglutinin of particular types and subtypes of influenza virus in polyvalent preparations. However, this test is time consuming therefore for hemagglutinin level determination ELISA test was adapted. This test is hundred times more sensitive and can be applied with success for determination of hemagglutinin level of influenza virus A or B. For evaluation of a degree of purification of vaccine preparations ELISA was elaborated, in which as an index of purification of preparation a level of ovalbumin is determined. This test is specific and extremely sensitive, and it is possible to determine ovalbumin level with accuracy of 1ng in 1 ml of preparation.

Animals↗
PubMed1990

[Respiratory viral infections in young children 1988-1990].

From October 1988 to June 1989 the studies on viral infections of respiratory tract were done in specimens taken from 461 children, aged 0-2 years. Similarly to the observations in previous epidemic seasons infections due to RS and parainfluenza type 3 viruses were dominated. These studies included also influenza type C and parainfluenza type 4 viruses with unknown epidemiology in Poland. The insignificant role of these infections in small children was estimated according to the low ratio of detection in specimens tested--0.4% and 0.9% respectively.

Child, Preschool↗
PubMed1990

[The role of respiratory tract viruses in the etiology of obstructive bronchitis in infants].

Out of 524 children with acute respiratory infections in 141 obstructive bronchitis was diagnosed (OZO). Seventy cases could be linked to viral infection. Viral infections tested (influenza virus A, B, parainfluenza typ 1-3, RSV, adenoviruses) were more frequently associated with OZO than other acute respiratory infections of unknown etiology. Majority infections induced by influenza virus A and parainfluenza virus typ 2 were accompanied by OZO symptoms. Of the highest risk of acquiring OZO despite of viral infection participation, were children of 4-12 months of age. OZO associated viral infections prevailed during autumn-winter season, while in spring-summer period undetermined factors were the major cause of OZO. In serum samples of children with OZO, despite of etiology of the disease, higher level of IgE was found than in a group of children without the symptoms. In the case of OZO of unestablished etiology the level of serum IgE was significantly higher than in the cases when viral etiology of the disease was found.

Adenoviruses, Human↗
PubMed1989

[Respiratory viral infections in young children 1985-1988].

In the period from May 1985 to June 1988 the authors (using the immunofluorescence method) examined 848 children aged 0-2 years hospitalized due to infections of respiratory tracts in the II Clinic of Pediatrics of the Pediatrics Department of the II Medical Faculty in the Medical Academy in Warsaw. The most frequent causes of infections in respiratory tracts were virus RS (21.1% of patients) and virus of parainfluenza type 3 (6.5% of patients). An increase in viral respiratory infections took place every year between early autumn and late spring. Virus RS was permanently present in the population examined, though significant increases in the number of children infected by that virus appeared from March to May and from October to January in every year of the examination. An increased incidence of type 3 parainfluenza virus infections usually appeared in September. Among the children examined, the authors also found 25 cases of simultaneous infections by two different viruses. The most frequent combination of infecting viruses were virus RS and virus of parainfluenza type 3.

Adenoviridae Infections↗
PubMed1987

Comparison of cytomegalovirus antibodies by means of CF, ELISA and immunoblotting techniques.

Antibody to human cytomegalovirus in sera of immune blood donors reacted with 12 viral polypeptides of molecular weight 200.000. 150.000, 65.000, 48.000, 34,000. 25.000, 23.000, 21.000 and 18.000. Development of CMV infection in renal transplant recipients resulted in increase of antibody reacting with various CMV polypeptides as well as in intensity of this reaction. The highly comparable results for CMV antibody detecting were obtained with immunoblotting and ELISA techniques.

Antibodies, Viral↗