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Biomedical subjects

M J Kelly

Publications and source records attributed to M J Kelly.

At least 217 records · Page 12Linked to original sources
PubMed1978

Counts on viable Bacteroides fragilis: a modification of the microdroplet technique.

A modification of Sharpe's microdroplet counting technique is presented which permits viable counting of Bacteroides fragilis. Lysed horse blood was added to 0.1 soy digest agar droplets and 2% was found to be the optimal concentration which combined adequate intradroplet colony size with acceptable visibility on the counting screen of the Colworth Droplette machine.

Bacteriological Techniques↗
PubMed1978

The quantitative and histological demonstration of pathogenic synergy between Escherichia coli and Bacteroides fragilis in guinea-pig wounds.

Known numbers of standard strains of Escherichia coli and Bacteroides fragilis were inoculated together into surgical incision in guinea-pigs. Histological and quantitative bacteriological proof is presented that pathogenic synergy occurred in vivo with copious pus formation when combinations of subinfective doses of each organism were inoculated. A further series of experiments showed that the amounts and ratios of E. coli and B. fragilis in the septic inocula were critical, and that the resultant pus contained equal numbers of both the aerobic and the anaerobic organisms. This model may be suitable for the experimental validation of short-term regimens of prophylactic antibiotics in surgery; the present results seem to have important therapeutic implications.

Animals↗
PubMed1978

The effect of ovariectomy on the responsiveness of preoptic-septal neurons to microelectrophoresed estrogen.

Extracellular potentials were recorded from medical preoptic-septal (MPOA-S) neurons in 2-week and 3--4 month ovariectomized rats, and the effects of microelectrophoresed 17beta-estradiol hemisuccinate (17betaE2S), acetylcholine (Ach), cortisol hemisuccinate (CS) and glutamate (Glut) were studied. 17betaE2S failed to produce changes in the firing rate of the majority of MPOA-S neurons tested, but Ach excited 48% of these neurons. MPOA-S neurons showed very little response to either Glut or CS. Previous findings from this laboratory have demonstrated that MPOA-S neurons recorded in 4-day cyclic female rats will respond to 17betaE2S. Therefore, the present results suggest that the ovarian steroids are necessary for maintaining this electrical sensitivity. Also, the involvement of a specific membrane or cytoplasmic receptor is discussed.

Acetylcholine↗
PubMed1977

Aerobic and anaerobic mixtures of human pathogens: a rapid 4-plate counting technique.

A reliable, cheap, labour-saving technique is presented for counting viable organisms in mixtures of aerobic and anaerobic human pathogenic bacteria. Series of 0.1-ml blood agar droplets containing antibiotics are used and only 4 Petri dishes are required. A system of controls makes recognition of any errors of technique particularly simple whilst allowing direct subculture of any unexpected colonies.

Aerobiosis↗
PubMed1977

Catatonia and malignant syndrome: a possible complication of neuroleptic administration. Report of a case involving haloperidol.

An unusual, acute extrapyramidal reaction, which resulted from treatment with haloperidol and which was unresponsive to standard anticholinergic treatment and indistinguishable on clinical grounds from acute catatonia, is described. Because the etiology of this reaction was not appreciated, progressive, life-threatening physiological deterioration ensued accompanied by unremitting neuromuscular symptoms, a clinical picture resembling both "lethal catatonia" and the rare "neuroleptic malignant syndrome." The literature on these conditions is reviewed and various problems in differential diagnosis are discussed. It is proposed that some cases of the neuroleptic malignant syndrome and perhaps lethal catatonia may represent the evolution of severe extrapyramidal reactions.

Adult↗
PubMed1976

Suicide attempts by hospitalized medical and surgical patients.

To characterize siucidal behavior among hospitalized medical and surgical patients, all suicide attempts in the Peter Bent Brigham Hospital were surveyed for seven years. Seventeen attempts occurred, non of them fatal. Only four patients were seriously ill, two with neoplasia. All the attempts were impulsive and were associated with stress and disturbances of impulse control. Anger, not depression, was the effect most often seen before the attempts. In all cases the precipitating stress was loss of emotional support. However, patient vulnerability to suicide seemed to be the key determinant. Fifteen patients had mental disorders, including eight with personality disorders, three with schizophrenia, three with organic brain syndromes, and one with manic depressive psychosis. Seven were psychotic, and six had made prior suicide attempts. These findings suggest that the characteristics of impulsive suicide should be considered when a suicide prevention program is being developed for a general hospital.

Adult↗
PubMed1976

Radiologic evaluation of the patient with suspected pulmonary thromboembolic disease.

The risks of anticoagulant and surgical therapy for pulmonary thromboembolic disease make a high degree of diagnostic certainty imperative. The pitfalls inherent in basing the diagnosis on clinical and laboratory information are discussed, and the radiologic and isotopic studies available to the clinician are presented, with special emphasis on their sensitivity and specificity and guidelines for their intelligent use.

Adult↗