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Biomedical subjects

M Itoh

Publications and source records attributed to M Itoh.

At least 1,081 records · Page 60Linked to original sources
PubMed1983

Enzyme immunoassay of HBeAg employing beta-D-galactosidase.

An enzyme-linked immunosorbent assay (ELISA) system for hepatitis B e antigen (HBeAg) was developed employing beta-D-galactosidase conjugated with antibody to HBeAg (anti-HBe) and using m-maleimidobenzoyl-N-hydroxysuccinimide ester as the coupling reagent. The experimental conditions for quantitative assay of HBeAg were determined. The presence of rheumatoid factor in test sera did not affect the results. This assay system is more sensitive than the micro-Ouchterlony method and as sensitive as radioimmunoassay. The use of beta-D-galactosidase for ELISA in the field of virology is recommended.

Galactosidases↗
PubMed1983

[Evaluation of cefotetan in obstetrics and gynecology].

Fundamental and clinical studies on gynecological use of cefotetan (CTT), a new cephamycin antibiotic, were performed with following results. Following the intravenous administration of 2.0 g of CTT, T 1/2 beta in serum was 3.1 hours and longer than the previous cephamycin antibiotics. The yields of CTT from serum to various uterine tissues and discharge from retroperitoneum were about 30 approximately 50%. In clinical use, 14 patients with gynecological infections were administrated CTT, and it showed excellent or good efficacy in all patients. No side effects were noted except 1 transient disturbance in liver function.

Adult↗
PubMed1983

[Adrenergic innervation and norepinephrine content in postnatal rat uterus].

Using fluorescent histochemical method and high performance liquid chromatography with electrochemical detector, we investigated adrenergic innervation and norepinephrine content in the rat uterus in the process of the growth. The adrenergic nerve terminals in the rat uterus developed with age and reached to adult level at 7 weeks of age after birth, although the short adrenergic ganglionic cells and small intense fluorescent cells were present even at birth. Norepinephrine content per organ also increased with age and reached to adult level at 10 weeks of age after birth, while NE content per gram wet tissue weight had a peak in 3-day-old rat uterus. These morphological and biochemical data revealed that the sympathetic nervous system in rat uterus matures in 7 to 10 weeks after birth, while the short adrenergic nervous system is accomplished in earlier stage. The maturation of adrenergic innervation in the uterus was considerably later than in the other organs of rat and developed with the sexual maturation.

Adrenergic Fibers↗
PubMed1982

Tryptophan fluorescence of human hemoglobin. II. Effect of inositol hexaphosphate on the T-R transition.

Fluorescence spectra of tryptophan residues of human hemoglobin in the absence and presence of inositol hexaphosphate were measured at room temperature. The tryptophan fluorescence intensity of deoxy HbA was observed to decrease in accordance with the binding with inositol hexaphosphate. The fluorescence intensity of HbA, Hb Kempsey (beta 99 Asp-Asn), Hb Chesapeake (alpha 92 Arg-Leu) and NES-des-Arg Hb (des-141 alpha Arg and beta 93 Cys-N-ethylsuccinimide derivative) in the presence of inositol hexaphosphate exhibits a considerable decrease in the deoxy to oxy transition, while no or slight fluorescence intensity change was observed in the deoxy to oxy transition of Hb Kempsey and NES-des-Arg Hb in the absence of inositol hexaphosphate. The tryptophan fluorescence behavior suggest that the inositol hexaphosphate-induced structural change in these hemoglobins is attributable to the formation of a different T type of structure from that of the normal T-R transition.

Hemoglobin A↗
PubMed1982

The selective inhibition of hippocampal glutamic acid decarboxylase in zinc-induced epileptic seizures.

The intracerebroventricular administration of Zn2+ (0.3 mumol/10 microliters) causes epileptic seizures characterized by running fits, jumping, vocalization, fasiculation of facial muscles, myoclonic movements of the limbs and tonic-clonic convulsions. These episodes are blocked or reversed by gamma-aminobutyric acid (0.4 mumol/10 microliters). When assayed under conditions where pyridoxal phosphate was not added, the activity of glutamic acid decarboxylase decreased significantly in hippocampus from 18.9 to 15.3 and 9.7 mumols 14CO2 formed/gram proteins/20 min, 15 and 30 min following administration of Zn2+. The inhibition of glutamic acid decarboxylase by Zn2+ was selective occurring only in hippocampus and not in the hypothalamus, amygdala, caudate or thalamus. The inhibition of glutamic acid decarboxylase was not due to a reduction in the concentration of endogenous pyridoxal phosphate which remained unaltered in hippocampus following Zn2+ administration.

Animals↗
PubMed1982

Adrenergic modulation of pancreatic somatostatin, insulin, and glucagon secretion: evidence for differential sensitivity of islet A, B, and D cells.

To characterize the adrenergic effects of epinephrine on somatostatin, insulin and glucagon release and to assess the potential interactions of islet A, B, and D cell function, isolated rat islets were incubated in vitro with epinephrine (0.05-20 microM) in the presence and absence of the alpha adrenergic antagonist, phentolamine (2 or 4 microM), and/or the beta adrenergic antagonist, propranolol (2 microM). At concentrations of epinephrine at or less than 1 microM, somatostatin and insulin release were inhibited while glucagon release was unaffected. At greater epinephrine concentrations, somatostatin and glucagon release were increased while insulin release was further suppressed. The threshold as well as the half-maximal effect of epinephrine occurred at lower concentrations for somatostatin release than for insulin and glucagon release. The inhibitory effect of 0.5 microM epinephrine on somatostatin and insulin release was completely reversed by phentolamine and was unaffected by propranolol. The stimulatory effect of 2 and 20 microM epinephrine on somatostatin and glucagon release was not observed when propranolol was included in the incubation medium along with epinephrine. These results demonstrate that rat islet A, B, and D cells differ in their sensitivity to alpha and beta adrenergic effects. At low concentrations of epinephrine, alpha adrenergic effects on D cells predominate over beta adrenergic effects whereas at greater concentrations of epinephrine alpha and beta effects appear to be equal; alpha adrenergic effects of epinephrine predominate over those of beta on the B cell at least up to 20 microM epinephrine: exclusively beta adrenergic effects of epinephrine are observed on the A cell at least up to 20 microM epinephrine.

Animals↗
PubMed1982

Preservation and visualization of actin-containing filaments in the apical zone of cochlear sensory cells.

The fine filamentous structure in the apical zone of cochlear sensory cells of the guinea pig was investigated under transmission electron microscopy (TEM) using various fixation methods. The true form of this structure, which is that of a dense core of sensory hairs and cuticular plates containing hair rootlets, has been hitherto unknown because of the selectively destructive effect of ordinary fixatives. We revealed the fine filamentous structure in great detail by fixing the specimens in tannic acid or by the modified glutaraldehyde--osmium fixation method, which can preserve action filaments during the procedures required to prepare the specimen for TEM. The filamentous structure gives the impression of a negatively stained image when prepared in this way. Filaments were packed regularly and tightly into dense cores which projected down deep into the cuticular plate as hair rootlets. Cross-striations were seen at intervals of 360 +/- 28 A along the packed filaments, a distance which is comparable to the periodicity of an actin paracrystal. The overall diameter of each filament was 83 A. In fact, the structure of dense cores and hair rootlets proved to be composed of actin paracrystals, probably containing some regulatory proteins. Cross-sectioned actin filaments in the paracrystal were arranged in an extremely regular hexagonal pattern. The characteristic filamentous texture in the cuticular plate was best seen in tissues that were pretreated with EDTA, and then fixed by tannic acid. It is probable that the greater part of the cuticular plate is composed of actin filaments and actin monomers, both containing Ca2+-dependent regulatory proteins. Utilizing the above ultrastructural findings, some functional models of this zone are proposed.

Actins↗
PubMed1982

Ultrastructure of peripheral lymphocytes in generalized ceroid-lipofuscinosis. Report of a case.

Electron microscopic observation was made on the peripheral blood cells obtained from a 4-year-old boy with generalized ceroid-lipofuscinosis (GCL), and two kinds of significant inclusions were found in the cytoplasm of lymphocytes. One was ceroid-lipofuscin accompanied with the fingerprint pattern, and the other was a lamellar body which showed the concentric lamellar structure or the myelin figure. Ceroid-lipofuscin was found in 40% of peripheral lymphocytes. It is important in diagnosing GCL that ceroid-lipofuscin can be detected with high incidence in lymphocytes of peripheral blood.

Ceroid↗