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Biomedical subjects

M Itoh

Publications and source records attributed to M Itoh.

At least 1,063 records · Page 59Linked to original sources
PubMed1984

[Histopathological study on the vascular invasion in advanced gastric carcinomas].

Venous and lymphatic invasion and Ag-reactive cells were studied in 110 gastric carcinomas. Venous invasion was recognized in 28.2% by H.E. staining only; it was seen in 58.2% by E.V.G. staining. Argyrophil and argentaffin cells were recognizable in 27.3% and 14.5%, respectively. No venous invasion was observed in many carcinomas with Ag-reactive cells. It has been suggested that the incidence of carcinoma with Ag-reactive cells is in inverse proportion to cellular atypism. Our work suggests that venous invasion bears a relation to atypism; this tendency was not recognizable with respect to lymphatic invasion.

Adenocarcinoma↗
PubMed1984

2-Aminodipyrido[1,2-a:3',2'-d]imidazole-porphyrin(Fe) derivatives as sequence specific DNA cleaving reagents.

Some porphyrin (Fe)-intercalators were synthesized. We chose 2-aminodipyrido[1, 2-a:3',2'-d]imidazoles (Glu-P's), which were muta-carcinogens isolated from a pyrolysate of L-glutamic acid, as intercalator moieties. These synthetic porphyrin (Fe)-intercalators cleave DNA at G-C and G-T sequences. The specificity for cleavage of base sequences of DNA is quite similar to that of bleomycin.

Aminopyridines↗
PubMed1984

[Quality control of radioimmunoassay--evaluation of individual components of error and their contribution rates to total error].

Using Insulin RIA Kit (double antibody method, Dainabot Co., Ltd.), individual components of error due to pipetting of reagents, bound-free separation and counting were evaluated experimentally and theoretically. They were statistically synthesized to get the total response error relationship (RER). The resulting RER agreed well with the pooled RER obtained by the method reported previously. This good agreement indicates that individual components of error were reasonably and precisely estimated. Based on this result, contribution rates of individual components of error to the total response error were evaluated. The averaged contribution rates over the range to measure, when pipetting precision, C.V. (coefficient of variation) = 0.005, were 64% for counting error, 24% for bound-free separation error, and the error related with reagent delivery was only 12%. When C.V. = 0.01, however, which is considered normal pipetting precision, the contribution rate for the latter went up to 35%. Effect of poor precision in pipetting on final assay results is so great that much more attention than we usually think of should be paid to pipettes and pipetting techniques.

Quality Control↗
PubMed1983

Effect of haloperidol on glutamate decarboxylase activity in discrete brain areas of the rat.

Using freeze-dried samples of rat brain, the effect of haloperidol on glutamate decarboxylase (GAD) activity without exogenously added pyridoxal-5'-phosphate (PLP) was studied in discrete brain nuclei and areas. Repeated injections of haloperidol produced significant changes in GAD activity in the dorsal part of the caudate nucleus, entopeduncular nucleus, pars reticulata of the substantia nigra, lateral hypothalamic area, and dorsomedial hypothalamic nucleus. A reduction of GAD activity after haloperidol was observed in the entopeduncular nucleus and pars reticulata of the substantia nigra. This finding demonstrates biochemically that haloperidol-induced extrapyramidal behavior may be involved in the reduction of GABAergic transmission in the entopeduncular nucleus and pars reticulata of the substantia nigra. A decrease in GAD activity in the lateral hypothalamic area indicates that interaction between GABAergic neurons as well as dopaminergic neurons may be involved in the haloperidol-induced behavioral changes. In addition, close interaction between GABAergic and dopaminergic systems in the dorsomedial hypothalamic nucleus and dorsal part to the caudate nucleus was demonstrated.

Animals↗
PubMed1983

Urodynamic studies on hemiplegic patients after cerebrovascular accident.

Thirty-nine hemiplegic patients were evaluated by complete urodynamic study after cerebrovascular accidents. Computerized tomography was performed to identify localization of the brain lesion. The common urinary symptoms were frequency and urgency incontinence (26/39), while 13 patients complained of dysuria or urinary retention. Ten of the 11 patients who had frontal and internal capsular lesions showed hyperactive bladder, and 6 showed uninhibited sphincter relaxation. Nine of the 10 patients who had putaminal lesions showed hyperactive bladder. Normal sphincter activity was demonstrated in 7 of these 10 patients. In the remaining patients with the other types of lesions, no correlation was found between urodynamic dysfunction and type of brain injury.

Aged↗
PubMed1983

Speech sound errors in patients with conduction and Broca's aphasia.

Speech sound errors exhibited by three conduction and three Broca's aphasic patients on naming and word-repetition tasks were subjected to phonemic and subphonemic analyses. In the conduction aphasic patients, errors occurred equally often on consonants and vowels in both the naming and word-repetition tasks, while in the Broca's aphasic patients errors occurred selectively on consonants. Transposition errors occurred almost as often as substitution errors in the conduction aphasic patients, while substitution errors constituted the majority of errors in the Broca's aphasic patients. The Broca's aphasic patients, as compared to the conduction aphasic patients, exhibited a markedly higher number of substitution errors occurring between phonemes separated by a single subphonemic feature on the naming task. On the basis of these findings, it was hypothesized that the differences in the error patterns of the two types of aphasia reflected differences in the underlying mechanisms of the impairment in each type.

Adult↗
PubMed1983

Velar Movements during speech in two Wernicke aphasic patients.

The articulatory gestures of the velum in two Wernicke aphasic patients were examined to compare their performances with those of an apraxic patient by means of the fiberoptic technique. In contrast to the marked variability in the apraxic performance in terms of velum height and segmental duration, the two fluent aphasic subjects showed a relatively high degree of consistency in velar movements throughout several repetitions of nonsense syllables and meaningful words. In addition, both patients exhibited a normal pattern of anticipatory coarticulation. Analyses of the velar movement patterns during the speech-sound error processes of both patients suggested that these errors were not due to an impairment at the level of articulatory programming but to an error in the selection of a target phoneme.

Aged↗
PubMed1983

The presence of zinc-binding proteins in brain.

Zinc is one of the most abundant divalent metal ions in the brain, its concentration being greater than those of copper and manganese. Since free zinc ion is a potent inhibitor of sulfhydryl enzymes, we postulated that zinc in the brain most probably exists bound to macromolecules. As zinc-binding proteins in brain have not been characterized, we attempted to discover the occurrence and properties of these proteins. By using Sephadex G-75 column chromatography calibrated with proteins of known molecular weights, and by other techniques, we detected separate zinc-binding proteins, with apparent estimated molecular weights ranging from 15,000 to 210,000. Unlike the hepatic or renal zinc thioneins, the zinc-binding proteins in brain are not inducible following administration of zinc. Our interpretation of the results is that the major portion of the existing zinc in the brain is bound, and does not exist in free form.

Animals↗
PubMed1983

Response of plasma somatostatin-like immunoreactivity (SLI) to a 75 g oral glucose tolerance test in normal subjects and patients with impaired glucose tolerance.

To study the role of somatostatin in the pathophysiology of glucose intolerance in man, plasma somatostatin-like immunoreactivity (SLI) was measured in 8 normal subjects, 6 patients with insulin dependent diabetes mellitus (IDDM), 13 with non-insulin dependent diabetes mellitus (NIDDM), and 9 with hyperthyroidism, by extraction of plasma SLI and radioimmunoassay. The extraction method gave a recovery rate for synthetic somatostatin-14 and somatostatin-28 of 72 +/- 6 and 55 +/- 7%, respectively. No SLI corresponding to somatostatin-28 in human peripheral blood was observed. Incubation of somatostatin-28 in plasma gave a rapid decrease of immunoreactivity, and no conversion to somatostatin-14 was observed. It is speculated that SLI extracted with acid-acetone mainly represents a molecular weight similar to somatostatin-14. After oral administration of glucose (75 g), a clear and sustained rise in plasma SLI was seen in normal subjects from an initial value (+/- SEM) of 29.9 +/- 5.4 pg/ml to a peak value, at 60 min of 93.4 +/- 15.5 pg/ml. The increase of plasma SLI after 75 g glucose was also observed in IDDM and NIDDM. The peak level of SLI was significantly less than that for normal subjects. The extraction of plasma SLI with acetic acid and acetone gave reproducible results and showed a fluctuation of SLI with glucose concentration.

Adult↗