Biomedical subjects

M Iijima

Publications and source records attributed to M Iijima.

At least 253 records · Page 14Linked to original sources

PubMed1979

[The incidences of spontaneous fetal anomalies in Japanese white rabbits (author's transl)].

The incidences of spontaneous fetal anomalies in coccidium-free Japanese white rabbits bred and kept in our laboratories are reported. Live fetuses were removed from 87 (95.6%) out of 91 maternal animals examined, totaling 538 (295 males and 243 females). of them, 9 fetuses (1.7%) showed external and visceral abnormalities such as hydrocephalia (5 cases), short tail (2), anophthalmia (1) and talipomanus flexa (1). Skeletal abnormalities were found in 7 (1.7%) of 424 fetues examined.

Animals↗

PubMed1979

[Bilateral internuclear ophthalmoplegia (author's transl)].

A case is reported of a patient with bilateral internuclear ophtalmoplegia which followed a single limited lesion of the two medial longitudinal fasciculi (M.L.F.), with degeneration of the right internal nucleus. The authors feel that the exact localization of this minute lesions, associated with right internal nucleus degeneration, can assist in supplying replies to some unanswered questions concerning the M.L.F. syndrome.

Brain↗

PubMed1978

Mode of action of pyocin R1.

The effects of pyocin Rl on transport systems and syntheses of macromolecules in Pseudomonas aeruginosa strain P14 were studied under two conditions, 0.4 mM and 40 mM Magnesium ions. In the presence of 0.4 mM magnesium ions, the incorporation of [14C]leucine into protein and the uptake of [14C]leucine into the leucine pool were completely inhibited by pyocin. Furthermore, [14C]leucine which had been taken up into the leucine pool was depleted by pyocin. Comparing the concentration of amino acid within cells with that in the medium, it was concluded that the active transport in P14 cells was inhibited completely by pyocin. Such an effect of pyocin Rl on transport systems was not specific to leucine but common to many amino acids and uridine. On the other hand, under the condition of 40 mM magnesium ions, [14C]-leucine once accumulated was not depleted by pyocin, but uptake of [14C]leucine into the leucine pool stopped immediately while incorporation of [14C]leucine into protein continued at a considerably reduced rate; therefore the machinery for protein synthesis was not directly inhibited by pyocin. It is suggested that the loss from the amino acid pool in cells brings about the rapid cessation of protein synthesis under the condition of 0.4 mM magnesium.

Bacterial Proteins↗

PubMed1978

[Nature of the erythroid colony stimulating extract obtained from spleen of irradiated rats (author's transl)].

Non-adherent bone marrow cells of a bled rabbit were cultured in plasma clot media containing auto-serum, alpha-medium, erythropoietin (Ep) and spleen extract from irradiated rats. The preparations were cloted on a cover glass, fixed and stained by Giemsa or hemoglobin staining method after 3 or 5 days in culture, and the number of erythroid colony was counted as reported elsewhere. In the present study, first, it was elucidated that the optimal numbers of innoculating cells were among 0.6 approximately 1.2 x 10(4) cells per well for the erythroid colony formation. Second, this colony formation was slightly stimulated by the experimental media which contained heat treated extract at 40 degree or 50 degree C for 30 minutes. Contrary this, the extract treated at 70 degree C for 30 minutes lost completely its stimulating activity of the colony formation, suggesting that the effective substances might be protein in the extract. Third, an inhibitory factory might be present in the dialysate of the crude spleen extract, because the number of erythroid colonies decreased in a dose response manner by the dialysate. The residue of inner dialysate, however, certainly contained the colony stimulating factors (s). The crude extract was separated into five fractions (F1 approximately F5) by ammonium sulfate. F1, which was precipitated with 40% ammonium sulfate, had the highest activity for the colony formation. Fetuin also showed appreciable effect on the erythroid colony formation.

Animals↗

PubMed1977

A study of hypertension: participation of central adrenaergic mechanism on the angiotensin II-induced hypertension.

I. Interactions of angiotensin II and norepinephrine in the rat hypothalamus and other brainstem were studied by intraventricular perfusion or/and intravenous administration. (1) Intravenous administration of angiotensin reduced the hypothalamus norepinephrine content. (2) Intraventricularly perfused angiotensin reduced the hypothalamus norepinephrine but did not alter that in other parts of brainstem. Hypothalamus norepinephrine was reduced more by intravenous administration of angiotensin than by intraventricularly perfused angiotensin. II. The involvements of adrenergic mechanism in the central pressor effect of angiotensin II were studied through observation of blood pressure changes following injection of angiotensin II, phentolamine and propranolol into the rabbit lateral ventricle of rabbit brain.(1) Intraventricular administration of phentolamine suppressed the central mediated pressor respons of angiotensin. (2) Simultaneous intraventricular administration of propranolol enhanced the angiotensin pressor effect in anesthetized rabbits.

Angiotensin II↗

PubMed1977

[Study of erythroid colony formation of rabbits in vitro: the effect of spleen extract from irradiated rats on CFU-E (author's transl)].

The rabbit was bled to activate the erythropoiesis, and the serum was stocked for culture. After two days, non-adherent bone marrow cells of the same rabbit were havested, and cultured consequently with auto-plasma and auto-serum, which had been prepared, alpha-medium, erythropoietin (Ep) and spleen extract derived from rats sublethally irradiated 8 days before. The FB-16-24-TC (Limbro Co.) was used for culturing bessel by the addition of round cover-glass on the bottom of each well. The preparations were made by the routine Giemsa and hemoglobin stain methods on 3rd, 5th and 9th day of culture. The erythroid and non-erythroid colonies were calcurated under the microscope with x200 or higher magnifications. Small erythroid colonies consisted of 8 approximately 16 cells were scattered on the 3rd day, and undifferentiated or granulocytic colonies also frequently appeared on the specimen. On the 5th day, medium size colonies consisted of 32 approximately 64 cells were observed. The most effective irradiation for active extract was 700R. On the other hand, if the culture medium did not contain the spleen extract, the number of colonies decreased. Other results showed that this extract have hardly any enhancement on colony formation without Ep in the medium. Therefore, it is probable that the extract may have contained the potentiators for the erythroid, as well as for the myeloid progenitors. Whether these spleen factors are the same or not as that reported by Knospe et al. or Morley et al. is unknown. The study is in progress.

Animals↗

PubMed1976

Effect of angiotensin II on noradrenaline content in the rat hypothalamus.

1. Intravenous administration of angiotensin II reduced the adrenaline content, increased the catechol-O-methyltransferase activity, and decreased the monoamine oxidase activity of rat hypothalamus. 2. Intraventricularly administrated angiotensin II reduced the noradreanline content to a smaller extent. 3. The change produced by intravenous administration of angiotensin might be indirectly caused by a response to angiotensin.

Angiotensin II↗

PubMed1976